Team:Warsaw/Calendar-Main/19 September 2008
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+ | <h3>MutD<sub>5</sub> testing</h3> | ||
+ | <h4>Piotr</h4> | ||
+ | <p>Testing MutD<sub>5</sub> ampicillin resistance - MutD<sub>5</sub> isn't resistant to ampicillin</p> | ||
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<h3>'Hunter and prey' system tests: Competition tests</h3> | <h3>'Hunter and prey' system tests: Competition tests</h3> | ||
- | <h4> | + | <h4>Emilia</h4> |
- | <p> | + | <ol><li><p>Samples of LB (0.25 mM IPTG; ampicillin 50 μl/ml) was inoculated with following strain combinations:</li> |
- | + | <ul><li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a> - A_alpha protein added ~100 μg </li> | |
- | < | + | <li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a> - Z_alpha protein added ~100 μg</li> |
- | <li> | + | <li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a> - Z_omega protein added ~100 μg</li> </ul></p> |
- | <li> | + | </li><li>Bacteria centrifuged and frozen |
- | </li><li>Bacteria centrifuged and | + | |
</li></ol> | </li></ol> | ||
- | <h3>Preparation of | + | <h3>Preparation of <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_K103003>ΔA (BBa_K103003)</a></h3> |
- | <h4>Michał K.</h4> | + | <h4>Michał K., Piotr</h4> |
<ol> | <ol> | ||
<li> Gel electrophoresis to estimate concentration of purified DNA from previous day. </li> | <li> Gel electrophoresis to estimate concentration of purified DNA from previous day. </li> | ||
- | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments of | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> and ΔA. </li><li> <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">transformation</a> with the above ligation. </li> |
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<li>Plating on LB + ampicillin.</li> | <li>Plating on LB + ampicillin.</li> |
Latest revision as of 21:14, 29 October 2008
MutD5 testingPiotrTesting MutD5 ampicillin resistance - MutD5 isn't resistant to ampicillin 'Hunter and prey' system tests: Competition testsEmilia
Preparation of ΔA (BBa_K103003)Michał K., Piotr
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