Team:NTU-Singapore/Notebook/5 July 2008
From 2008.igem.org
(Difference between revisions)
Lalala8585 (Talk | contribs) |
Lalala8585 (Talk | contribs) |
||
(One intermediate revision not shown) | |||
Line 3: | Line 3: | ||
<div id="header">{{User:Greenbear/sandbox/header}}</div> | <div id="header">{{User:Greenbear/sandbox/header}}</div> | ||
- | <div id="maincontent" style="margin-top: | + | <div id="maincontent" style="margin-top:130px;"> |
<html> | <html> | ||
<div id="arrow"> | <div id="arrow"> | ||
Line 26: | Line 26: | ||
**Nanodrop to determine concentrations and purities of LacI-RBS 34 plasmids, which show good results. | **Nanodrop to determine concentrations and purities of LacI-RBS 34 plasmids, which show good results. | ||
**Prepare digestion mixtures for gel confirmation. | **Prepare digestion mixtures for gel confirmation. | ||
- | + | ===Darius=== | |
+ | :Gel electrophoresis to check if there are LacI-RBS 34 plasmids.<br>Result: All have the 2000 kb band, the empty vector plasmid (expected). Besides that band: | ||
**There is 1 sample that only has 250 bp band, which is LacI-RBS 34. (Good!!) | **There is 1 sample that only has 250 bp band, which is LacI-RBS 34. (Good!!) | ||
**There is 2 samples that have both 250 bp band and less than 50 bp band, which can be interpreted as RBS (due to self-ligation of RBS). | **There is 2 samples that have both 250 bp band and less than 50 bp band, which can be interpreted as RBS (due to self-ligation of RBS). | ||
**The rest only have the RBS band. | **The rest only have the RBS band. | ||
</div> | </div> |
Latest revision as of 02:24, 28 October 2008
|
Contents |
Saturday 5 July
Lu Chao
Hung, Lu Chao
Darius
|