Team:Warsaw/Calendar-Main/4 July 2008

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Latest revision as of 14:07, 28 October 2008


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Isolation of plasmids from transformants.
Control digest of plasmids with NotI.
Gel electrophoresis of digested DNA. There where proper clones, but they weren't red or green in UV (the reason might be low copy number of the plasmid or something else).

No sucess in changing of the reporter from pZC320 with B-galactosidase to GFP or RFP.

Isolation of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega).
Control digest of plasmids with SacI and BamHI (BamHI buffer).
Gel electrophoresis.

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 <html>
-<h3>Changing of the reporter from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> with B-galactosidase to GFP or RFP</h3>
+<h3>Change of the reporter from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> with B-galactosidase to GFP or RFP</h3>
 <h4>Piotr, Weronika, Emilia</h4>
 <ol>
 <li><a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from transformants.</li>
 <li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest"> digest</a> of plasmids with NotI.</li>
-<li>Gel electrophoresis of digested DNA there where proper plasmids, but they weren't red or green in UV (the reason might be low number of plasmid copies or something else)</li>
+<li>Gel electrophoresis of digested DNA. There where proper clones, but they weren't red or green in UV (the reason might be low copy number of the plasmid or something else).</li>
@@ Line 19: / Line 19: @@
-<h3>Preparation of constructs with OmpA protein fusions</h3>
+<h3>Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega</h3>
 <h4>Paweł</h4>
 <p><ol>