Team:Warsaw/Calendar-Main/19 September 2008

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<h3>MutD<sub>5</sub> testing</h3>
<h3>MutD<sub>5</sub> testing</h3>
<h4>Piotr</h4>
<h4>Piotr</h4>
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Testing MutD<sub>5</sub> ampicillin resistance - MutD<sub>5</sub> has not spontaneous ampicillin resistance mutations.
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<p>Testing MutD<sub>5</sub> ampicillin resistance - MutD<sub>5</sub> isn't resistant to ampicillin</p>
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<h3>'Hunter and prey' system tests: Competition tests</h3>
<h3>'Hunter and prey' system tests: Competition tests</h3>
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<h4>Weronika</h4>
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<h4>Emilia</h4>
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<p>
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<ol><li><p>Samples of LB (0.25 mM IPTG; ampicillin 50 μl/ml) was inoculated with following strain combinations:</li>
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Inoculation to one tube (0.25 mM IPTG; ampicillin 50 μl/ml)          
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<ul><li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a> - A_alpha protein added ~100 μg </li>  
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<ol><li><ul><li>Omp_A_omega, Omp_omega and Omp_Z_omega - A_alpha protein added ~100 μg </li>  
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<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a> - Z_alpha protein added ~100 μg</li>  
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<li>Omp_A_omega, Omp_omega and Omp_Z_omega - Z_alfa protein added ~100 μg</li>  
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<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a> - Z_omega protein added ~100 μg</li> </ul></p>
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<li>Omp_A_alpha, Omp_alpha and Omp_Z_alpha - Z_omega protein added ~100 μg</li> </ul></p>
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</li><li>Bacteria centrifuged and frozen
</li><li>Bacteria centrifuged and frozen
</li></ol>
</li></ol>
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<h3>Preparation of BioBricks</h3>
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<h3>Preparation of <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_K103003>&Delta;A (BBa_K103003)</a></h3>
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<h4>Michał K.</h4>
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<h4>Michał K., Piotr</h4>
<ol>
<ol>
<li> Gel electrophoresis to estimate concentration of purified DNA from previous day. </li>
<li> Gel electrophoresis to estimate concentration of purified DNA from previous day. </li>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments of RFP(??????) and deltaA. </li></ol>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> and &Delta;A. </li><li> <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">transformation</a> with the above ligation. </li>
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<h3></h3>
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<h4>Piotr</h4>
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<ol><li> <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">transformation</a> with the above ligation. </li>
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<li>Plating on LB + ampicillin.</li>
<li>Plating on LB + ampicillin.</li>

Latest revision as of 21:14, 29 October 2008

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MutD5 testing

Piotr

Testing MutD5 ampicillin resistance - MutD5 isn't resistant to ampicillin

'Hunter and prey' system tests: Competition tests

Emilia

  1. Samples of LB (0.25 mM IPTG; ampicillin 50 μl/ml) was inoculated with following strain combinations:

  2. Bacteria centrifuged and frozen

Preparation of ΔA (BBa_K103003)

Michał K., Piotr

  1. Gel electrophoresis to estimate concentration of purified DNA from previous day.
  2. Ligation of isolated DNA fragments of pSB1A3 and ΔA.
  3. E. coli TOP10 transformation with the above ligation.
  4. Plating on LB + ampicillin.

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