Team:Warsaw/Calendar-Main/22 October 2008

From 2008.igem.org

(Difference between revisions)
(New page: {{WarNotebook}} <!-- do not edit above me! --> <html> <h3>Preparation of BioBricks</h3> <h4>Michał K.</h4> <ol> </html> <!-- do not remove this! --> {{WarNotebookEnd}})
 
(17 intermediate revisions not shown)
Line 4: Line 4:
<html>
<html>
-
<h3>Preparation of BioBricks</h3>
+
 
 +
 
 +
 
 +
<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103002>AID under pBAD/araC (BBa_K103002)</a></h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>
<ol>
<ol>
 +
<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day - <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> + <a href=http://partsregistry.org/Part:BBa_K103002>AID under pBAD/araC (BBa_K103002)</a>.</li>
 +
<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found. We didn't manage to obtain this BioBrick.</li></ol>
-
</html>
+
<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103014>OmpA_linker_alpha_linker (BBa_K103014)</a></h3>
 +
<h4>Michał K.</h4>
 +
<ol>
 +
<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day - <a href=http://partsregistry.org/Part:pSB2K3>pSB2K3</a> + <a href=<a href=http://partsregistry.org/Part:BBa_K103014>OmpA_linker_alpha_linker (BBa_K103014)</a>.</li>
 +
<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found. <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/22_October_2008#fig1">Fig. 1</a>.</li></ol>
 +
<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/4/44/Traw_22_10_2008.jpg"></a>
 +
<var><b>Fig.1.</b>Control EcoRI/PstI digests of isolated plasmids.<br>
 +
1. Marker<br>
 +
2-9. EcoRI/PstI digests of isolated plasmids<br></var>
 +
<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a></h3>
 +
<h4>Piotr</h4>
 +
<ol>
 +
<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day <a href=http://partsregistry.org/Part:BBa_I739204>pACYC177</a> + <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a>.</li>
 +
<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.</li>
 +
</ol>
 +
 +
</html>
<!-- do not remove this! -->
<!-- do not remove this! -->
{{WarNotebookEnd}}
{{WarNotebookEnd}}

Latest revision as of 15:25, 28 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



Preparation of AID under pBAD/araC (BBa_K103002)

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day - pSB1A3 + AID under pBAD/araC (BBa_K103002).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found. We didn't manage to obtain this BioBrick.

Preparation of OmpA_linker_alpha_linker (BBa_K103014)

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day - pSB2K3 + OmpA_linker_alpha_linker (BBa_K103014).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found. Fig. 1.
Fig.1.Control EcoRI/PstI digests of isolated plasmids.
1. Marker
2-9. EcoRI/PstI digests of isolated plasmids

Preparation of OmpA-linker-omega-linker (BBa_K103016)

Piotr

  1. Isolation of plasmids from cultures inoculated on previous day pACYC177 + OmpA-linker-omega-linker (BBa_K103016).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/22_October_2008"