Team:Chiba/Calendar-Home/20 October 2008
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>[[Team:Chiba|Home]] | [[Team:Chiba/Notebook|Notebook]] | >[[Team:Chiba|Home]] | [[Team:Chiba/Notebook|Notebook]] | ||
- | [[Team:Chiba/Calendar-Home/ | + | [[Team:Chiba/Calendar-Home/19 October 2008|19 October 2008 <]]|[[Team:Chiba/Calendar-Home/21 October 2008|> 21 October 2008]] |
- | ===Team: | + | |
+ | ==Laboratory work== | ||
+ | ===Team:Receivers=== | ||
Transformation | Transformation | ||
- | #BBa_T9002(sensitive mutant LuxR) | + | #[http://partsregistry.org/Part:BBa_T9002%E3%80%80BBa_T9002 BBa_T9002][https://2008.igem.org/Team:Chiba/Experiments:LuxR_mutant (sensitive mutant LuxR)] |
- | # | + | #[http://partsregistry.org/Part:BBa_T9002%E3%80%80BBa_T9002 BBa_T9002] |
- | #BBa_T9002(RBS0.6) | + | #[http://partsregistry.org/Part:BBa_T9002%E3%80%80BBa_T9002 BBa_T9002](RBS0.6) |
#Plac-his aiiA-T | #Plac-his aiiA-T | ||
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Ptet-RBS-luxR-T-Plux-RBS-GFP-Cm-p15A | Ptet-RBS-luxR-T-Plux-RBS-GFP-Cm-p15A | ||
- | #Ptet-RBS-luxR-T-Plux-RBS-GFP-Cm-p15A | + | #[https://2008.igem.org/Team:Chiba/Experiments:copy_number Ptet-RBS-luxR-T-Plux-RBS-GFP-Cm-p15A] |
#Ptet-RBS-LuxR-Kan-P15A | #Ptet-RBS-LuxR-Kan-P15A | ||
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#Ptet-RBS-LuxR-T-Plux-RBS-GFP-T-Plac-aiiA-Amp-colE1 | #Ptet-RBS-LuxR-T-Plux-RBS-GFP-T-Plac-aiiA-Amp-colE1 | ||
+ | |||
+ | -->[[Team:Chiba/protocol/transformation|Transformation]] | ||
<BR> | <BR> | ||
- | |||
- | |||
- | |||
+ | ===Team:Demo-Rs=== | ||
+ | <BR> | ||
+ | #Receiver(T9002) pre-incubation | ||
+ | ##Receiver:BBa_T9002(JW1908)wascultured in 2mL LB-Amp (37°C,12h) | ||
- | + | ~21 October | |
- | + |
Latest revision as of 01:49, 30 October 2008
19 October 2008 <|> 21 October 2008
Laboratory work
Team:Receivers
Transformation
- [http://partsregistry.org/Part:BBa_T9002%E3%80%80BBa_T9002 BBa_T9002](sensitive mutant LuxR)
- [http://partsregistry.org/Part:BBa_T9002%E3%80%80BBa_T9002 BBa_T9002]
- [http://partsregistry.org/Part:BBa_T9002%E3%80%80BBa_T9002 BBa_T9002](RBS0.6)
- Plac-his aiiA-T
Ptet-RBS-luxR-T-Plux-RBS-GFP-Cm-p15A
- Ptet-RBS-LuxR-Kan-P15A
Plux-RBS-GFP-T-Amp-colE1
- Ptet-RBS-LuxR-T-Plux-RBS-GFP-T-Plac-aiiA-Amp-colE1
Team:Demo-Rs
- Receiver(T9002) pre-incubation
- Receiver:BBa_T9002(JW1908)wascultured in 2mL LB-Amp (37°C,12h)
~21 October