Team:Chiba/Parts

From 2008.igem.org

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<html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Hiroki/style.css&action=raw&ctype=text/css" type="text/css" /></html>
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<html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html>
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[[Image:Chiba-U.gif]]
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[[Image:Chiba-U.gif|center]]
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__NOTOC__
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{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |
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{| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
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!align="center"|[[Team:Chiba|Home]]
!align="center"|[[Team:Chiba|Home]]
!align="center"|[[Team:Chiba/Team|The Team]]
!align="center"|[[Team:Chiba/Team|The Team]]
Line 11: Line 10:
!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
|}
|}
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==We hope to submit these parts==
+
__NOTOC__
 +
==We submitted these parts==
===Autoinducer synthase===
===Autoinducer synthase===
==== parts usages ====
==== parts usages ====
-
*[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
+
*[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0178 LasI(no LVA)])
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:[http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0178 LasI(no LVA)]
+
[[Image:Tet-lasi chiba.gif]]
-
日本語:IPTGにより、シュードモナス由来の3OC12HSL合成酵素,LasIを発現するデバイス。
+
LasI (3OC12HSL synthesizing protein from ''Pseudomonas aeruginosa'') expression induced by IPTG.
-
English:
+
*[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/Part:BBa_C0170 RhlI(no LVA)]) and [http://partsregistry.org/Part:BBa_K084009 BBa_K084009]([http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9026 rbs+RhlI])
 +
[[Image:Tet-rhli chiba.gif]]
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*[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
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RhlI (C4HSL,C6HSL synthesizing protein from ''Pseudomonas aeruginosa'') expression induced by IPTG.
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:[http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/Part:BBa_C0170 RhlI(no LVA)]
+
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*[http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
+
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:[http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9026 rbs+RhlI]
+
-
日本語:IPTGにより、シュードモナス由来のC4HSL,C6HSL合成酵素,RhlIを発現するデバイス。
+
*[http://partsregistry.org/Part:BBa_K084010 BBa_K084010]([http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9030 rbs+CinI])
-
English:
+
CinI (3-hydroxy-C14HSL synthesizing protein from ''Rhizobium leguminosarum'') expression induced by IPTG.
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*[http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
 
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:[http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9026 rbs+RhlI]
 
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*[http://partsregistry.org/Part:BBa_K084010 BBa_K084010]
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*[http://partsregistry.org/Part:BBa_K084012 BBa_K084012]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0161 LuxI(no LVA)]) and [http://partsregistry.org/Part:BBa_K084014 BBa_K084014]([http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0261 rbs+LuxI])
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:[http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9030 rbs+CinI]
+
[[Image:Tet-luxi chiba.gif]]
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日本語:IPTGにより、Rhizobium leguminosarum由来のC14HSL合成酵素,CinIを発現するデバイス。
+
Lux I (3OC6HSL synthesizing protein from ''Vibrio Fischeri'') expression induced by IPTG.
-
English:
+
===Transcriptional Regulator===
 +
*[http://partsregistry.org/Part:BBa_K084004 BBa_K084004]  ([http://partsregistry.org/Part:BBa_R0040  BBa_R0040]+[http://partsregistry.org/Part:BBa_S03158 BBa_S03158])
 +
[[Image:RhlR-p15A Chiba.gif]]
 +
Transcriptional regulator RhlR (BBa_C0071) generator regulated by tet promoter
 +
*[http://partsregistry.org/Part:BBa_K084005 BBa_K084005]  ([http://partsregistry.org/Part:BBa_R0040  BBa_R0040]+[http://partsregistry.org/Part:BBa_S03156 BBa_S03156])
 +
[[Image:LasR-p15A Chiba.gif]]
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*[http://partsregistry.org/Part:BBa_K084012 BBa_K084012]
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Transcriptional regulator LasR (BBa_C0079) generator regulated by tet promoter
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:[http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0161 LuxI(no LVA)]
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*[http://partsregistry.org/Part:BBa_K084006 BBa_K084006]
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*[http://partsregistry.org/Part:BBa_K084014 BBa_K084014]
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([http://partsregistry.org/Part:BBa_R0040  BBa_R0040]+[http://partsregistry.org/Part:BBa_S03160 BBa_S03160])
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:[http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0261 rbs+LuxI]
+
[[Image:CinR-p15A Chiba.gif]]
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日本語:IPTGにより、Vibrio Fischeri由来の3OC6HSL合成酵素,LuxIを発現するデバイス。
+
Transcriptional regulator CinR (BBa_C0077) generator regulated by tet promoter
-
English:
+
===Reporters===
 +
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003]
 +
[[Image:K084003 chiba.gif]]
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<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center>
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3OC6HSL -> Venus YFP  Venus YFP Producer Controlled by 3OC6HSL Receiver  
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<tr>
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<td width="200">Part Name</td>
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<td width="200">Sub Parts</td>
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<td width="200">Part Length(bp)<td>
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<td width="200">Plasmid Vector<td>
+
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<td width="300">Part Length(bp)<td>
+
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<td width="200">Part and vector length(bp)<td>
+
-
<td width="200">Resistance<td>
+
-
<td width="200">Status<td>
+
-
<td width="200">Sequenced<td>
+
-
<td width="200">Sent<td>
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-
 
+
-
</tr>
+
-
<tr>
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<td width="200">[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]</td>
+
-
<td width="300">[http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0178 LasI(no LVA)]</td>
+
-
<td width="200">835<td>
+
-
<td width="200">pSB1AK3<td>
+
-
<td width="200">3189<td>
+
-
<td width="200">4024<td>
+
-
<td width="200">Amp/Kan<td>
+
-
<td width="350">Glycerol stock in -80/miniprepped<td>
+
-
<td width="50">ok<td>
+
-
<td width="50">[[Image:Chiba check.png]]<td>
+
-
 
+
-
</tr>
+
-
<tr>
+
-
<td width="200">[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]</td>
+
-
<td width="300">[http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/Part:BBa_C0170 RhlI(no LVA)]</td>
+
-
<td width="200">835<td>
+
-
<td width="200">pSB1AK3<td>
+
-
<td width="200">3189<td>
+
-
<td width="200">4024<td>
+
-
<td width="200">Amp/Kan<td>
+
-
<td width="350">Glycerol stock in -80/miniprepped<td>
+
-
<td width="50">ok<td>
+
-
<td width="50">[[Image:Chiba check.png]]<td>
+
-
</tr>
+
-
<tr>
+
-
<td width="200">[http://partsregistry.org/Part:BBa_K084009 BBa_K084009]</td>
+
-
<td width="300">[http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9026 rbs+RhlI]</td>
+
-
<td width="200">893<td>
+
-
<td width="200">pSB1A2<td>
+
-
<td width="200">2079<td>
+
-
<td width="200">2972<td>
+
-
<td width="200">Amp<td>
+
-
<td width="350">Glycerol stock in -80/miniprepped<td>
+
-
<td width="50">ok<td>
+
-
<td width="50">[[Image:Chiba check.png]]<td>
+
-
</tr>
+
-
<tr>
+
-
<td width="200">[http://partsregistry.org/Part:BBa_K084010 BBa_K084010]</td>
+
-
<td width="300">[http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9030 rbs+CinI]</td>
+
-
<td width="200">953<td>
+
-
<td width="200">pSB1A2<td>
+
-
<td width="200">2079<td>
+
-
<td width="200">3032<td>
+
-
<td width="200">Amp<td>
+
-
<td width="350">Glycerol stock in -80/miniprepped<td>
+
-
<td width="50">ok<td>
+
-
<td width="50">[[Image:Chiba check.png]]<td>
+
-
</tr>
+
-
<tr>
+
-
<td width="200">[http://partsregistry.org/Part:BBa_K084012 BBa_K084012]</td>
+
-
<td width="300">[http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0161 LuxI(no LVA)]</td>
+
-
<td width="200">811<td>
+
-
<td width="200">pSB1AK3<td>
+
-
<td width="200">3189<td>
+
-
<td width="200">4000<td>
+
-
<td width="200">Amp/Kan<td>
+
-
<td width="350">Glycerol stock in -80/miniprepped<td>
+
-
<td width="50">Not yet<td>
+
-
<td width="50">[[Image:Chiba check.png]]<td>
+
-
</tr>
+
-
<tr>
+
-
<td width="200">[http://partsregistry.org/Part:BBa_K084014 BBa_K084014]</td>
+
-
<td width="300">[http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0261 rbs+LuxI]</td>
+
-
<td width="200">869<td>
+
-
<td width="200">pSB1A2<td>
+
-
<td width="200">2079<td>
+
-
<td width="200">2948<td>
+
-
<td width="200">Amp<td>
+
-
<td width="350">Glycerol stock in -80/miniprepped<td>
+
-
<td width="50">Not yet<td>
+
-
<td width="50">[[Image:Chiba check.png]]<td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
===Receiver protein===
+
-
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center>
+
-
<tr>
+
-
<td width="200">Part Name</td>
+
-
<td width="200">Sub Parts</td>
+
-
<td width="200">Part Length(bp)<td>
+
-
<td width="200">Plasmid Vector<td>
+
-
<td width="300">Part Length(bp)<td>
+
-
<td width="200">Part and vector length(bp)<td>
+
-
<td width="200">Resistance<td>
+
-
<td width="200">Status<td>
+
-
<td width="200">Sequenced<td>
+
-
<td width="200">Sent<td>
+
-
</tr>
+
-
<tr>
+
-
<td width="200">[http://partsregistry.org/Part:BBa_K084004 BBa_K084004]</td>
+
-
<td width="300">ptet+rbs+RhlR</td>
+
-
<td width="200">867<td>
+
-
<td width="200">pSB1A2<td>
+
-
<td width="200">2079<td>
+
-
<td width="200">2946<td>
+
-
<td width="200">Amp<td>
+
-
<td width="350">miniprepped<td>
+
-
<td width="50">ok<td>
+
-
<td width="50">[[Image:Chiba check.png]]<td>
+
-
</tr>
+
-
<tr>
+
-
<td width="200">[http://partsregistry.org/Part:BBa_K084004 BBa_K084004]</td>
+
-
<td width="300">ptet+rbs+RhlR</td>
+
-
<td width="200">867<td>
+
-
<td width="200">pSB3K3<td>
+
-
<td width="200">2750<td>
+
-
<td width="200">3617<td>
+
-
<td width="200">Kan<td>
+
-
<td width="350">miniprepped<td>
+
-
<td width="50">Not yet<td>
+
-
<td width="50">[[Image:Chiba check.png]]<td>
+
-
</tr>
+
-
<tr>
+
-
<td width="200">[http://partsregistry.org/Part:BBa_K084005 BBa_K084005]</td>
+
-
<td width="300">ptet+rbs+LasR</td>
+
-
<td width="200">861<td>
+
-
<td width="200">pSB1A2<td>
+
-
<td width="200">2079<td>
+
-
<td width="200">2940<td>
+
-
<td width="200">Amp<td>
+
-
<td width="350">miniprepped<td>
+
-
<td width="50">ok<td>
+
-
<td width="50">[[Image:Chiba check.png]]<td>
+
-
</tr>
+
-
<tr>
+
-
<td width="200">[http://partsregistry.org/Part:BBa_K084005 BBa_K084005]</td>
+
-
<td width="300">ptet+rbs+LasR</td>
+
-
<td width="200">861<td>
+
-
<td width="200">pSB3K3<td>
+
-
<td width="200">2750<td>
+
-
<td width="200">3611<td>
+
-
<td width="200">Kan<td>
+
-
<td width="350">miniprepped<td>
+
-
<td width="50">Not yet<td>
+
-
<td width="50">[[Image:Chiba check.png]]<td>
+
-
</tr>
+
-
<tr>
+
-
<td width="200">[http://partsregistry.org/Part:BBa_K084006 BBa_K084006]</td>
+
-
<td width="300">ptet+rbs+CinR</td>
+
-
<td width="200">867<td>
+
-
<td width="200">pSB1A2<td>
+
-
<td width="200">2079<td>
+
-
<td width="200">2946<td>
+
-
<td width="200">Amp<td>
+
-
<td width="350">miniprepped<td>
+
-
<td width="50">ok<td>
+
-
<td width="50">[[Image:Chiba check.png]]<td>
+
-
</tr>
+
-
<tr>
+
-
<td width="200">[http://partsregistry.org/Part:BBa_K084006 BBa_K084006]</td>
+
-
<td width="300">ptet+rbs+CinR</td>
+
-
<td width="200">867<td>
+
-
<td width="200">pSB3K3<td>
+
-
<td width="200">2750<td>
+
-
<td width="200">3617<td>
+
-
<td width="200">Kan<td>
+
-
<td width="350">miniprepped<td>
+
-
<td width="50">Not yet<td>
+
-
<td width="50">[[Image:Chiba check.png]]<td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
===Reporters===
+
-
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center>
+
-
<tr>
+
-
<td width="200">Part Name</td>
+
-
<td width="200">Sub Parts</td>
+
-
<td width="200">Part Length(bp)<td>
+
-
<td width="200">Plasmid Vector<td>
+
-
<td width="300">Part Length(bp)<td>
+
-
<td width="200">Part and vector length(bp)<td>
+
-
<td width="200">Resistance<td>
+
-
<td width="200">Status<td>
+
-
<td width="200">Sequenced<td>
+
-
<td width="200">Sent<td>
+
-
</tr>
+
-
<tr>
+
-
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084002 BBa_K084002]</td>
+
-
<td width="300">rbs+venusYFP(yeast optimized)</td>
+
-
<td width="200">767<td>
+
-
<td width="200"><td>
+
-
<td width="200"><td>
+
-
<td width="200"><td>
+
-
<td width="200"><td>
+
-
<td width="350">miniprepped<td>
+
-
<td width="50"><td>
+
-
<td width="50"><td>
+
-
</tr>
+
-
<tr>
+
-
<td width="200">[http://partsregistry.org/Part:BBa_K084003 BBa_K084003]</td>
+
-
<td width="300">ptet+LuxR+plux+venusYFP(yeast optimized)</td>
+
-
<td width="200">1836<td>
+
-
<td width="200">pSB1A2<td>
+
-
<td width="200">2079<td>
+
-
<td width="200">3915<td>
+
-
<td width="200">Amp<td>
+
-
<td width="350">miniprepped<td>
+
-
<td width="50">OK<td>
+
-
<td width="50">[[Image:Chiba check.png]]<td>
+
-
</tr>
+
-
</table>
+
-
 
+
 +
==== [[Team:Chiba/parts/parts status|parts status]] ====
-
{| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
+
{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |
!align="center"|[[Team:Chiba|Home]]
!align="center"|[[Team:Chiba|Home]]
!align="center"|[[Team:Chiba/Team|The Team]]
!align="center"|[[Team:Chiba/Team|The Team]]

Latest revision as of 01:21, 30 October 2008

Chiba-U.gif
Home The Team The Project Parts Submitted to the Registry Reference Notebook Acknowledgements

We submitted these parts

Autoinducer synthase

parts usages

  • [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0178 LasI(no LVA)])

Tet-lasi chiba.gif

LasI (3OC12HSL synthesizing protein from Pseudomonas aeruginosa) expression induced by IPTG.

  • [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/Part:BBa_C0170 RhlI(no LVA)]) and [http://partsregistry.org/Part:BBa_K084009 BBa_K084009]([http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9026 rbs+RhlI])

Tet-rhli chiba.gif

RhlI (C4HSL,C6HSL synthesizing protein from Pseudomonas aeruginosa) expression induced by IPTG.

  • [http://partsregistry.org/Part:BBa_K084010 BBa_K084010]([http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9030 rbs+CinI])

CinI (3-hydroxy-C14HSL synthesizing protein from Rhizobium leguminosarum) expression induced by IPTG.


  • [http://partsregistry.org/Part:BBa_K084012 BBa_K084012]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0161 LuxI(no LVA)]) and [http://partsregistry.org/Part:BBa_K084014 BBa_K084014]([http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0261 rbs+LuxI])

Tet-luxi chiba.gif

Lux I (3OC6HSL synthesizing protein from Vibrio Fischeri) expression induced by IPTG.

Transcriptional Regulator

  • [http://partsregistry.org/Part:BBa_K084004 BBa_K084004] ([http://partsregistry.org/Part:BBa_R0040 BBa_R0040]+[http://partsregistry.org/Part:BBa_S03158 BBa_S03158])

RhlR-p15A Chiba.gif

Transcriptional regulator RhlR (BBa_C0071) generator regulated by tet promoter

  • [http://partsregistry.org/Part:BBa_K084005 BBa_K084005] ([http://partsregistry.org/Part:BBa_R0040 BBa_R0040]+[http://partsregistry.org/Part:BBa_S03156 BBa_S03156])

LasR-p15A Chiba.gif

Transcriptional regulator LasR (BBa_C0079) generator regulated by tet promoter

  • [http://partsregistry.org/Part:BBa_K084006 BBa_K084006]

([http://partsregistry.org/Part:BBa_R0040 BBa_R0040]+[http://partsregistry.org/Part:BBa_S03160 BBa_S03160]) CinR-p15A Chiba.gif

Transcriptional regulator CinR (BBa_C0077) generator regulated by tet promoter

Reporters

  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003]

K084003 chiba.gif

3OC6HSL -> Venus YFP Venus YFP Producer Controlled by 3OC6HSL Receiver

parts status

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