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Montreal/Notebook/May
From 2008.igem.org
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| + | !style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal|<font color="#ffffff">Home</font>]] | ||
| + | !style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal/Team|<font color="#ffffff">The Team</font>]] | ||
| + | !style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal/Project|<font color="#ffffff">The Project</font>]] | ||
| + | !style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal/Parts|<font color="#ffffff">Parts Submitted to the Registry</font>]] | ||
| + | !style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal/Modeling|<font color="#ffffff">Modeling</font>]] | ||
| + | !style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal/Notebook|<font color="#ffffff">Notebook</font>]] | ||
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| - | color: black;">[[Montreal/May|May 2008]]</td> | + | color: black;">[[Montreal/Notebook/May|May 2008]]</td> |
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| - | <td>[[Montreal/May#May_22|22]]</td> | + | <td>[[Montreal/Notebook/May#May_22|22]]</td> |
| - | <td>[[Montreal/May#May_23|23]]</td> | + | <td>[[Montreal/Notebook/May#May_23|23]]</td> |
| - | <td>[[Montreal/May#May_24|24]]</td> | + | <td>[[Montreal/Notebook/May#May_24|24]]</td> |
| - | <td>[[Montreal/May#May_25|25]]</td> | + | <td>[[Montreal/Notebook/May#May_25|25]]</td> |
| - | <td>[[Montreal/May#May_26|26]]</td> | + | <td>[[Montreal/Notebook/May#May_26|26]]</td> |
| - | <td>[[Montreal/May#May_27|27]]</td> | + | <td>[[Montreal/Notebook/May#May_27|27]]</td> |
| - | <td>[[Montreal/May#May_28|29]]</td> | + | <td>[[Montreal/Notebook/May#May_28|29]]</td> |
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| - | <td>[[Montreal/May#May_30|30]]</td> | + | <td>[[Montreal/Notebook/May#May_30|30]]</td> |
| - | <td>[[Montreal/May#May_31|31]]</td> | + | <td>[[Montreal/Notebook/May#May_31|31]]</td> |
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Diluted Reporter cells 1/1000 with 5ul Kan/mL culture for 16h incubation at 5:30pm. To be used for Maxiprep at 9:30am-1:30pm. | Diluted Reporter cells 1/1000 with 5ul Kan/mL culture for 16h incubation at 5:30pm. To be used for Maxiprep at 9:30am-1:30pm. | ||
| - | === May | + | === May 28 === |
Ran gel on Elowitz Reporter DNA cut with EcoR1; 2 bands | Ran gel on Elowitz Reporter DNA cut with EcoR1; 2 bands | ||
Latest revision as of 18:05, 17 June 2008
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook |
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Contents |
May 2008
May 22
Prepared TOP10 competent cells for eventual transformation. Performed Mini-prep on Reporter+ Cells Performed Digest and Gel on Reporter plasmid extract -- no DNA present, suggest follow-up maxi-prep
May 23
Transformed Top10 cells with Puc19 to ensure that the competent cell procedure was successful. Growth was observed, therefore procedure was successful.
May 25
Diluted Reporter cells 1/1000 with 5ul Kan/mL culture for 16h incubation at 5:30pm. To be used for Maxiprep at 9:30am-1:30pm.
May 28
Ran gel on Elowitz Reporter DNA cut with EcoR1; 2 bands 0.7 kb and 2.0 kb, confirms identity of reporter DNA. Seeded syn-I and J-40001 into amp/kan LB and kan LB.
May 29
Growth of J-brick in culture - No growth of I-brick on culture Seeded J-brick for Midi-Prep in 40mL LB with ampicillin
Transformed TOP10 cells with both I brick and Reporter Plasmid
