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Team:Warsaw/Calendar-Main/7 July 2008
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| - | <h3>Preparation of constructs | + | <h3>Preparation of constructs: OmpA_alpha and OmpA_omega #2</h3> |
<h4>Piotr, Weronika</h4> | <h4>Piotr, Weronika</h4> | ||
| - | <p> | + | <p>It's the second attempt to clone OmpA_alpha and OmpA_omega to pACYC177, but now thank's to Paweł we've got both fusions on pET15b.</p> |
<ol> | <ol> | ||
<li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of confirmed clones of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b+OmpA_omega</a> with NdeI and BamHI (Tango 2x buffer). </li> | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of confirmed clones of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b+OmpA_omega</a> with NdeI and BamHI (Tango 2x buffer). </li> | ||
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<li> Overnight <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177>pACYC177</a> and OmpA_omega. </li> | <li> Overnight <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177>pACYC177</a> and OmpA_omega. </li> | ||
</ol> | </ol> | ||
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<h3>Cloning omega-A fusion on pKS (second attempt)</h3> | <h3>Cloning omega-A fusion on pKS (second attempt)</h3> | ||
Latest revision as of 16:33, 26 October 2008
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Preparation of constructs: OmpA_alpha and OmpA_omega #2Piotr, WeronikaIt's the second attempt to clone OmpA_alpha and OmpA_omega to pACYC177, but now thank's to Paweł we've got both fusions on pET15b.
Cloning omega-A fusion on pKS (second attempt)Michał L., Ewa, MarcinWe have just received corrected version of omegaP-link10-homo2 primer. Let's hope this time everything will be fine. We are repeating the PCRs:
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