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Team:Warsaw/Calendar-Main/11 July 2008
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primers on colonies from plates with transformations OmpA_alpha (annealing temperature - 55°C,45 s of elongation step). </li> | primers on colonies from plates with transformations OmpA_alpha (annealing temperature - 55°C,45 s of elongation step). </li> | ||
| - | <li> Gel electrophoresis <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/11_July_2008#fig1">Fig. 1</a>.</li> | + | <li> Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/11_July_2008#fig1">Fig. 1.</a>).</li> |
<li> Confirmed transformant colonies inoculated to liquid LB with kanamycin.</li> | <li> Confirmed transformant colonies inoculated to liquid LB with kanamycin.</li> | ||
</ol></p> | </ol></p> | ||
| - | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/7/7e/11_th_july.jpg" width=300 /></a><var>Fig. 1. Colony PCR using: OmpaL_N and OmpaP_link primers<b></b><br> | + | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/7/7e/11_th_july.jpg" width=300 /></a><var><b>Fig. 1. </b>Colony PCR using: OmpaL_N and OmpaP_link primers<b></b><br> |
Upper<br> | Upper<br> | ||
1. Marker<br> | 1. Marker<br> | ||
Latest revision as of 17:44, 26 October 2008
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Preparation of construct pKS with A proteinMichał L., Ewa, Marcin
Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpAPiotr, AntoniIsolation of plasmids from cultures inoculated on previous day. Preparation of constructs: OmpA_alpha and OmpA_omega #2Michał K.
 Fig. 1. Colony PCR using: OmpaL_N and OmpaP_link primersUpper 1. Marker 2-13. colony PCR products carrying probable OmpA-alpha Lower 1. Marker 2-13. colony PCR products carrying probable OmpA-alpha |
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