Team:Chiba/Sender

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English:Each species has their own LuxI-type proteins,which synthesize their specific autoinducers,AHLs.AHLs produced by different LuxI-type proteins differ only in the length of the acyl-chain moiety and substitution at position C-3.LuxR,which is original for Vibrio fischeri,is activated by its cognate autoinducer,3OC6HSL.However,LuxR is also activated by non-endogenous molecules,C4HSL,C6HSL,and 3OC12HSL.Activation by non-endogenous molecules requires a higher signal concentration(2).This results in slower activation of receivers,when AHL concentration is increasing.
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<html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html>
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[[Image:Chiba-U.gif]]
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{| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
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!align="center"|[[Team:Chiba|Home]]
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!align="center"|[[Team:Chiba/Team|The Team]]
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!align="center"|[[Team:Chiba/Project|The Project]]
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!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Chiba/Reference|Reference]]
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!align="center"|[[Team:Chiba/Notebook|Notebook]]
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!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
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|}
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__NOTOC__
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==Signal molecule sender==
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===Design===
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[[Image:Sender switch Chiba.jpg|left|]]
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Each species has their own LuxI-type proteins,which synthesize their specific autoinducers,AHLs.AHLs produced by different LuxI-type proteins differ only in the length of the acyl-chain moiety and substitution at position C-3.LuxR,which is original for Vibrio fischeri,is activated by its cognate autoinducer,3OC6HSL.However,LuxR is also activated by non-endogenous molecules,C4HSL,C6HSL,and 3OC12HSL.Activation by non-endogenous molecules requires a higher signal concentration(2).This results in slower activation of receivers,when AHL concentration is increasing.
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[[Image:AHL variety chiba.gif|frame|'''Fig.4''' AHL varieties]]
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AHLsおよびその合成酵素
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<table width="500" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center>
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<tr>
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<td width="200">Strain</td>
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<td width="200">AHL</td>
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<td width="150">LuxI-type protein<td>
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</tr>
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<tr>
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<td>P.aeruginosa</td>
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<td>C4HSL, C6HSL</td>
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<td>RhlI</td>
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</tr>
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<tr>
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<td>V. fisheri</td>
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<td>3OC6HSL</td>
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<td>LuxI</td>
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</tr>
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<tr>
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<td>P.aeruginosa</td>
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<td>3OC12HSL</td>
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<td>LasI</td>
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</tr>
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</table>
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<br clear=all>
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===Experiment===
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{| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
 +
!align="center"|[[Team:Chiba|Home]]
 +
!align="center"|[[Team:Chiba/Team|The Team]]
 +
!align="center"|[[Team:Chiba/Project|The Project]]
 +
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]
 +
!align="center"|[[Team:Chiba/Reference|Reference]]
 +
!align="center"|[[Team:Chiba/Notebook|Notebook]]
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!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
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|}

Latest revision as of 18:32, 26 October 2008

Chiba-U.gif

Home The Team The Project Parts Submitted to the Registry Reference Notebook Acknowledgements

Signal molecule sender

Design

Sender switch Chiba.jpg

Each species has their own LuxI-type proteins,which synthesize their specific autoinducers,AHLs.AHLs produced by different LuxI-type proteins differ only in the length of the acyl-chain moiety and substitution at position C-3.LuxR,which is original for Vibrio fischeri,is activated by its cognate autoinducer,3OC6HSL.However,LuxR is also activated by non-endogenous molecules,C4HSL,C6HSL,and 3OC12HSL.Activation by non-endogenous molecules requires a higher signal concentration(2).This results in slower activation of receivers,when AHL concentration is increasing.

Fig.4 AHL varieties


AHLsおよびその合成酵素

Strain AHL LuxI-type protein
P.aeruginosa C4HSL, C6HSL RhlI
V. fisheri 3OC6HSL LuxI
P.aeruginosa 3OC12HSL LasI


Experiment

Home The Team The Project Parts Submitted to the Registry Reference Notebook Acknowledgements
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