"
Team:Chiba/Receiver
From 2008.igem.org
(Difference between revisions)
(New page: <html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html> Image:Chiba-U.gif {| style="color...) |
(→Design) |
||
| (6 intermediate revisions not shown) | |||
| Line 11: | Line 11: | ||
|} | |} | ||
__NOTOC__ | __NOTOC__ | ||
| + | == Signal Molecule Receiver== | ||
| + | |||
==Quorum_Sensing_Crosstalk== | ==Quorum_Sensing_Crosstalk== | ||
===Design=== | ===Design=== | ||
| + | [[Image:Table R chiba.gif|frame|left|Table.1 LuxR family gene]] | ||
| + | センダーとレシーバーを元来とは異なる組み合わせにすることによって、thresholdに達する時間を遅くする。（杉山） | ||
| + | |||
===Experiment=== | ===Experiment=== | ||
| - | |||
==Plasmid_Copy_number== | ==Plasmid_Copy_number== | ||
| + | ===Design=== | ||
| + | |||
===Experiment=== | ===Experiment=== | ||
== LuxR_mutant == | == LuxR_mutant == | ||
===Design=== | ===Design=== | ||
| + | [[Image:Location-of-the-mutant-in-LuxR Chiba.gif|frame|right|'''Fig.6''' Location of the LuxR mutant in LuxR]] | ||
| + | [[Image:Mutant-LuxR Chiba.gif|frame|right|'''Table.1''' Seisitivity of LuxR mutants. Data modified from Collins et.al. Mol. Microbiol. 55, 712–723 (2005)]] | ||
| + | |||
| + | |||
| + | Collins et.al. described the hyper-sensitive variants of luxR to AHL.(Collins, C. H., Arnold, F. H. & Leadbetter, J. R. Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones. Mol. Microbiol. 55, 712–723 (2005)) | ||
| + | |||
| + | 私たちはmutated Receiverを用いることで、AHL感受性の違う２種類（WTと変異体）のレシーバーを用意し、delay-timeのバリエーションを増やした。 | ||
| + | (小林) | ||
| + | |||
===Experiment=== | ===Experiment=== | ||
Latest revision as of 20:40, 27 October 2008
| Home | The Team | The Project | Parts Submitted to the Registry | Reference | Notebook | Acknowledgements |
|---|
Signal Molecule Receiver
Quorum_Sensing_Crosstalk
Design
Table.1 LuxR family gene
センダーとレシーバーを元来とは異なる組み合わせにすることによって、thresholdに達する時間を遅くする。（杉山）
Experiment
Plasmid_Copy_number
Design
Experiment
LuxR_mutant
Design
Fig.6 Location of the LuxR mutant in LuxR
Table.1 Seisitivity of LuxR mutants. Data modified from Collins et.al. Mol. Microbiol. 55, 712–723 (2005)
Collins et.al. described the hyper-sensitive variants of luxR to AHL.(Collins, C. H., Arnold, F. H. & Leadbetter, J. R. Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones. Mol. Microbiol. 55, 712–723 (2005))
私たちはmutated Receiverを用いることで、AHL感受性の違う２種類（WTと変異体）のレシーバーを用意し、delay-timeのバリエーションを増やした。 (小林)
Experiment
| Home | The Team | The Project | Parts Submitted to the Registry | Reference | Notebook | Acknowledgements |
|---|
