Team:Warsaw/Calendar-Main/19 August 2008
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Electroporation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with ligations products.</li> | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Electroporation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with ligations products.</li> | ||
- | <li> Transformants plating on LB + | + | <li> Transformants plating on LB + kanamycin.</li></ol> |
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<var><b>Fig. 1.</b>Digest product - pGeneart+Z (lanes 1-3), pKS+A (4-6), pET15b+OmpA-A (8-10). Lane 7 - GeneRuler™ DNA Ladder Mix</var> | <var><b>Fig. 1.</b>Digest product - pGeneart+Z (lanes 1-3), pKS+A (4-6), pET15b+OmpA-A (8-10). Lane 7 - GeneRuler™ DNA Ladder Mix</var> | ||
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- | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/4/4b/PKS%2BApGApET%2Balfa.JPG"></a> | + | <a name="fig1"><center><img src="https://static.igem.org/mediawiki/2008/4/4b/PKS%2BApGApET%2Balfa.JPG"></a> |
<var><b>Fig. 1.</b>Digest product - pGeneart+Z (lanes 1-3), pKS+A (4-6), pET15b+OmpA-A (8-10). Lane 7 - GeneRuler™ DNA Ladder Mix</var> | <var><b>Fig. 1.</b>Digest product - pGeneart+Z (lanes 1-3), pKS+A (4-6), pET15b+OmpA-A (8-10). Lane 7 - GeneRuler™ DNA Ladder Mix</var> | ||
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Latest revision as of 13:13, 29 October 2008
Tests for ampicillin resistance given by protein added to mediumPiotrInoculation of TOP10 carrying pACYC177+OmpA_A_alpha, pACYC177+OmpA_ΔA_alpha, pACYC177+OmpA_ΔA_omega, pACYC177+OmpA_A_omega, pACYC177+OmpA_omega_ΔA, pACYC177+OmpA_alpha, pACYC177+OmpA_omega and pACYC177+OmpA_omega_ΔA_alpha to LB with inductor (0.25 mM IPTG) and kanamycin. Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omegaMichał K.
Cloning of protein A DNA to pET15b+OmpA-alpha plasmidAntoni
Cloning of protein A DNA to GeneArt plasmidAntoni
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