Team:Warsaw/Calendar-Main/5 August 2008
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<h3>Checking if <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>OmpA_omega_ΔA_alpha</a> gives ampicillin resistance</h3> | <h3>Checking if <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>OmpA_omega_ΔA_alpha</a> gives ampicillin resistance</h3> | ||
- | <h4>Piotr</h4> | + | <h4>Piotr, Weronika</h4> |
<p>Inoculation <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>OmpA_omega_ΔA_alpha</a> from various IPTG concentrations (in mmol/mL) (0, 0.1, 0.25, 0.5, 0.75, 1) into same IPTG concentrations, but with various ampicillin concentrations (in mmol/mL)(25, 50, 75, 100) in ratio: 1:50.</p> | <p>Inoculation <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>OmpA_omega_ΔA_alpha</a> from various IPTG concentrations (in mmol/mL) (0, 0.1, 0.25, 0.5, 0.75, 1) into same IPTG concentrations, but with various ampicillin concentrations (in mmol/mL)(25, 50, 75, 100) in ratio: 1:50.</p> | ||
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Inoculation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>OmpA_omega_ΔA_alpha</a> into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75 mmol/mL (repetition is necessary)</p> | Inoculation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>OmpA_omega_ΔA_alpha</a> into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75 mmol/mL (repetition is necessary)</p> | ||
- | <h3>Checking <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>OmpA_omega_ΔA_alpha | + | <h3>Checking <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>OmpA_omega_ΔA_alpha</a> expression</h3> |
- | <h4>Piotr</h4> | + | <h4>Piotr, Weronika</h4> |
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<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/e/ea/Western1_WAW.jpg" width=400/></a> | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/e/ea/Western1_WAW.jpg" width=400/></a> | ||
- | <var><b>Fig. 1.Protein A detection in | + | <var><b>Fig. 1.Protein A detection in bacterial lysates.</b><br> |
- | 1 | + | 1 - protein marker,<br> |
- | + | 2 and 3 - <i>E coli</i> without plasmid,<br> | |
- | + | 4 - <b>Omp_omega_deltaA_alpha</b> + 0,5 mM IPTG,<br> | |
- | + | 5 - <i>E coli</i> without plasmid,<br> | |
- | + | 6 - <b>Omp_omega_deltaA_alpha</b> + 1 mM IPTG,<br> | |
+ | 7 - Omp_omega_A_deltaalpha without inducer.</var> | ||
<br><br> | <br><br> |
Latest revision as of 09:01, 29 October 2008
Cloning of truncated fragment of protein A (ΔA)Emilia
Checking if OmpA_omega_ΔA_alpha gives ampicillin resistancePiotr, WeronikaInoculation OmpA_omega_ΔA_alpha from various IPTG concentrations (in mmol/mL) (0, 0.1, 0.25, 0.5, 0.75, 1) into same IPTG concentrations, but with various ampicillin concentrations (in mmol/mL)(25, 50, 75, 100) in ratio: 1:50. Measurement of bacterial culture growth (OD) in the evening:
Inoculation of OmpA_omega_ΔA_alpha into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75 mmol/mL (repetition is necessary) Checking OmpA_omega_ΔA_alpha expressionPiotr, Weronika
1 - protein marker, 2 and 3 - E coli without plasmid, 4 - Omp_omega_deltaA_alpha + 0,5 mM IPTG, 5 - E coli without plasmid, 6 - Omp_omega_deltaA_alpha + 1 mM IPTG, 7 - Omp_omega_A_deltaalpha without inducer. Preparing pACYC177+OmpA_omega_ΔA constructMichał K.
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