Team:Rice University/Notebook/4 June 2008

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(Wednesday 4 June)
 
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***lambda DNA with XhoI and HindIII
***lambda DNA with XhoI and HindIII
***lambda DNA with HindIII only
***lambda DNA with HindIII only
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*Taylor Stevenson
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**Titered the phage stocks obtained from packaging the NEB lambda genome. See packaging manual [https://static.igem.org/mediawiki/2008/6/6a/Packaging_Extract.pdf].
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**'''Result'''-no colonies grew with a 1/200 (phage mixture:cell culture) was plated.  Additionally, the more diluted phage mixture showed a titer of approximately 10^5 pfu/uL.
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:Rice_University|Home]]
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!align="center"|[[Team:Rice_University/Team|The Team]]
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!align="center"|[[Team:Rice_University/Project|The Project]]
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!align="center"|[[Team:Rice_University/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Rice_University/Modeling|Modeling]]
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!align="center"|[[Team:Rice_University/Notebook|Notebook]]
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|}

Latest revision as of 20:40, 16 July 2008

Wednesday 4 June

  • Selim Sheikh:
    • Prepared 2 digestion solutions:
      • lambda DNA with XhoI and HindIII
      • lambda DNA with HindIII only
  • Taylor Stevenson
    • Titered the phage stocks obtained from packaging the NEB lambda genome. See packaging manual [1].
    • Result-no colonies grew with a 1/200 (phage mixture:cell culture) was plated. Additionally, the more diluted phage mixture showed a titer of approximately 10^5 pfu/uL.

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