Team:Rice University/Notebook/4 June 2008
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***lambda DNA with XhoI and HindIII | ***lambda DNA with XhoI and HindIII | ||
***lambda DNA with HindIII only | ***lambda DNA with HindIII only | ||
+ | *Taylor Stevenson | ||
+ | **Titered the phage stocks obtained from packaging the NEB lambda genome. See packaging manual [https://static.igem.org/mediawiki/2008/6/6a/Packaging_Extract.pdf]. | ||
+ | **'''Result'''-no colonies grew with a 1/200 (phage mixture:cell culture) was plated. Additionally, the more diluted phage mixture showed a titer of approximately 10^5 pfu/uL. | ||
+ | <BR<BR><BR><BR> | ||
+ | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" | ||
+ | !align="center"|[[Team:Rice_University|Home]] | ||
+ | !align="center"|[[Team:Rice_University/Team|The Team]] | ||
+ | !align="center"|[[Team:Rice_University/Project|The Project]] | ||
+ | !align="center"|[[Team:Rice_University/Parts|Parts Submitted to the Registry]] | ||
+ | !align="center"|[[Team:Rice_University/Modeling|Modeling]] | ||
+ | !align="center"|[[Team:Rice_University/Notebook|Notebook]] | ||
+ | |} |
Latest revision as of 20:40, 16 July 2008
Wednesday 4 June
- Selim Sheikh:
- Prepared 2 digestion solutions:
- lambda DNA with XhoI and HindIII
- lambda DNA with HindIII only
- Prepared 2 digestion solutions:
- Taylor Stevenson
- Titered the phage stocks obtained from packaging the NEB lambda genome. See packaging manual [1].
- Result-no colonies grew with a 1/200 (phage mixture:cell culture) was plated. Additionally, the more diluted phage mixture showed a titer of approximately 10^5 pfu/uL.
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