Team:ESBS-Strasbourg/Parts

From 2008.igem.org

(Difference between revisions)
(→Toolbox for modular transcription factors)
 
(12 intermediate revisions not shown)
Line 1: Line 1:
<div id="header">{{Template:Team:ESBS-Strasbourg/Templates/Header}}</div>
<div id="header">{{Template:Team:ESBS-Strasbourg/Templates/Header}}</div>
<div id="maincontent" style="margin-top:150px;">
<div id="maincontent" style="margin-top:150px;">
-
 
+
__notoc__
-
[[Team:ESBS-Strasbourg/Project|<= back]]
+
-
<br>
+
=Parts submitted=
=Parts submitted=
-
None yet... :(
+
Following you can find a summary of our contribution to the registry.
 +
==Toolbox for modular transcription factors==
 +
[[image:ESBS_TF.jpg|thumb|300px|right]]
 +
===DNA binding domains===
 +
* '''tetR''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105003 BBa_K105003]
 +
* '''cI''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105004 BBa_K105004]
 +
* '''lexA''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105005 BBa_K105005]
 +
* '''lacI''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105006 BBa_K105006]
 +
* '''Gal4''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105007 BBa_K105007]
 +
===Activation domain===
 +
* '''VP16''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105001 BBa_K105001]
 +
===Repression domain===
 +
* '''TUP1''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105002 BBa_K105002]
 +
===Fluorescent markers===
 +
* '''CFP''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105009 BBa_K105009]
 +
* '''mCherry''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105010 BBa_K105010]
 +
* '''mOrange''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105011 BBa_K105011]
 +
===Junctions===
 +
* '''linker''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105012 BBa_K105012]
 +
===Tags===
 +
* '''cin8 - cell cycle dependent degradation ''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105013 BBa_K105013]
 +
* '''hsl1 – cell cycle dependent degradation''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105015 BBa_K105015]
 +
<br>
 +
=> all these parts are in our new plasmid derivate [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105000 pSB1A2_fusion]
 +
<br>
 +
<br>
-
===Note===
+
==Toolbox for modular promoters==
 +
===Minimal promoters of different strength===
 +
* '''cyc100''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105027 BBa_K105027]
 +
* '''cyc70''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105028 BBa_K105028]
 +
* '''cyc43''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105029 BBa_K105029]
 +
* '''cyc28''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105030 BBa_K105030]
 +
* '''cyc16''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105031 BBa_K105031]
 +
===Operator sites===
 +
* '''tetR operator''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105020 BBa_K105020]
 +
* '''cI operator''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105021 BBa_K105021]
 +
* '''lexA operator''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105022 BBa_K105022]
 +
* '''lacI operator''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105023 BBa_K105023]
 +
* '''Gal4 operator''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105024 BBa_K105024]
 +
<br>
-
If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
+
==Yeast selection markers==
 +
* '''Leu2 - autotrophy marker''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105016 BBa_K105016]
 +
* '''Ura3 - autotrophy marker''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105017 BBa_K105017]
 +
<br>
 +
==Remakes of good, but not available BioBricks==
 +
* '''Gal1 promoter''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105026 BBa_K105026]
 +
* '''Adh1 terminator''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105025 BBa_K105025]
<br>
<br>

Latest revision as of 23:07, 29 October 2008


Parts submitted

Following you can find a summary of our contribution to the registry.

Toolbox for modular transcription factors

ESBS TF.jpg

DNA binding domains

  • tetR [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105003 BBa_K105003]
  • cI [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105004 BBa_K105004]
  • lexA [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105005 BBa_K105005]
  • lacI [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105006 BBa_K105006]
  • Gal4 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105007 BBa_K105007]

Activation domain

  • VP16 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105001 BBa_K105001]

Repression domain

  • TUP1 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105002 BBa_K105002]

Fluorescent markers

  • CFP [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105009 BBa_K105009]
  • mCherry [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105010 BBa_K105010]
  • mOrange [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105011 BBa_K105011]

Junctions

  • linker [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105012 BBa_K105012]

Tags

  • cin8 - cell cycle dependent degradation [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105013 BBa_K105013]
  • hsl1 – cell cycle dependent degradation [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105015 BBa_K105015]


=> all these parts are in our new plasmid derivate [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105000 pSB1A2_fusion]

Toolbox for modular promoters

Minimal promoters of different strength

  • cyc100 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105027 BBa_K105027]
  • cyc70 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105028 BBa_K105028]
  • cyc43 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105029 BBa_K105029]
  • cyc28 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105030 BBa_K105030]
  • cyc16 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105031 BBa_K105031]

Operator sites

  • tetR operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105020 BBa_K105020]
  • cI operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105021 BBa_K105021]
  • lexA operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105022 BBa_K105022]
  • lacI operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105023 BBa_K105023]
  • Gal4 operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105024 BBa_K105024]


Yeast selection markers

  • Leu2 - autotrophy marker [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105016 BBa_K105016]
  • Ura3 - autotrophy marker [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105017 BBa_K105017]


Remakes of good, but not available BioBricks

  • Gal1 promoter [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105026 BBa_K105026]
  • Adh1 terminator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105025 BBa_K105025]

Retrieved from "http://2008.igem.org/Team:ESBS-Strasbourg/Parts"