Team:Warsaw/Calendar-Main/22 October 2008

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<h3>Preparation of BioBricks</h3>
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<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103002>AID under pBAD/araC (BBa_K103002)</a></h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>
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<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day pACYC177 + OmpA_alpha_ and RFP(?????) + AraC+pBAD+AID.</li>
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<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day - <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> + <a href=http://partsregistry.org/Part:BBa_K103002>AID under pBAD/araC (BBa_K103002)</a>.</li>
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<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found only for pACYC177 + OmpA_alpha_.</li></ol>
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<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found. We didn't manage to obtain this BioBrick.</li></ol>
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<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103014>OmpA_linker_alpha_linker (BBa_K103014)</a></h3>
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<h4>Michał K.</h4>
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<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day - <a href=http://partsregistry.org/Part:pSB2K3>pSB2K3</a> + <a href=<a href=http://partsregistry.org/Part:BBa_K103014>OmpA_linker_alpha_linker (BBa_K103014)</a>.</li>
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<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found. <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/22_October_2008#fig1">Fig. 1</a>.</li></ol>
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<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/4/44/Traw_22_10_2008.jpg"></a>
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<var><b>Fig.1.</b>Control EcoRI/PstI digests of isolated plasmids.<br>
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1. Marker<br>
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2-9. EcoRI/PstI digests of isolated plasmids<br></var>

Latest revision as of 15:25, 28 October 2008

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Preparation of AID under pBAD/araC (BBa_K103002)

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day - pSB1A3 + AID under pBAD/araC (BBa_K103002).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found. We didn't manage to obtain this BioBrick.

Preparation of OmpA_linker_alpha_linker (BBa_K103014)

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day - pSB2K3 + OmpA_linker_alpha_linker (BBa_K103014).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found. Fig. 1.
Fig.1.Control EcoRI/PstI digests of isolated plasmids.
1. Marker
2-9. EcoRI/PstI digests of isolated plasmids

Preparation of OmpA-linker-omega-linker (BBa_K103016)

Piotr

  1. Isolation of plasmids from cultures inoculated on previous day pACYC177 + OmpA-linker-omega-linker (BBa_K103016).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

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