From 2008.igem.org
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| - | <big><b><u>Making LB Media</u></b></big> <br>
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| - | <b>Materials </b><br>
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| - | 500 mL media bottle or rack of test tubes <br>
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| - | 1 L beaker <br>
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| - | Magnetic stir bar <br>
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| - | Tryptone <br>
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| - | Yeast extract<br>
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| - | Sodium chloride <br>
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| - | Granulate agar (only for solid media) <br>
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| - | <b>Instructions </b><br>
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| - | 1. Fill 1 L beaker with 500mL of deionized water.<br>
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| - | 2. Add magetic stir bar into beaker and on to magnetic stirrer. Turn to medium speed. <br>
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| - | 3. Measure and add slowly with a scupula: <br>
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| - | - 5g tryptone <br>
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| - | - 2.5g yeast extract <br>
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| - | - 2.5 g sodium chloride <br>
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| - | 4. Once dissolved in the deionized water, pour in media bottle or aliquot 5mL in tubes.<br>
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| - | 5. If making solid media, add 7.5g granulated agar to the media bottle. DO NOT add it to the beaker. <br>
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| - | 6. Autoclave slightly open bottle/tubes on slow exchange.<br>
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Latest revision as of 21:16, 28 October 2008
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