Team:Hawaii/Notebook/2008-06-19

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===Alkaline Lysis Miniprep===
===Alkaline Lysis Miniprep===
:<strong> Krystle & Margaret </strong>
:<strong> Krystle & Margaret </strong>
 +
:* Plasmid prep was performed according to the experimental protocol
 +
:* Plasmid prep was performed with:
 +
 +
{| border="1"
 +
|+ '''BioBricks'''
 +
!part number
 +
!antibx media strength
 +
!source organism
 +
|-
 +
|E0040 (GFP)
 +
|rowspan= "5" align="center" |amp100
 +
|DH5&alpha
 +
|-
 +
|J04430 (lac+GFP)
 +
|DH5&alpha
 +
|-
 +
|B0034 (RBS)
 +
|GP's super competent cells
 +
|-
 +
|B0024 (TT)
 +
|DH5&alpha
 +
|}
 +
 +
 +
{| border="1"
 +
|+ '''Tool Plasmids'''
 +
! type
 +
!antibx media strength
 +
!source organism
 +
|-
 +
|align="center" |pUC18
 +
|rowspan= "2" align="center" | amp100
 +
|rowspan= "2" align="center" |DH5&alpha
 +
|-
 +
|align="center" |pBLUSCRIPT
 +
|-
 +
|align="center" |RP1 
 +
|align="center" |kan50 
 +
|align="center" |E. coli
 +
|}
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:* Plasmid prep was performed with Biobricks (E0040 (GFP) with amp100 from DH5alpha, J04430 (lac+GFP) with amp100 from DH5alpha, B0034 (RBS) with amp100 from GP's super competent cells, and B0024 (TT)with amp100 from DH5alpha) and plasmids (pUC18 with amp100 from DH5alpha, pBLUSCRIPT with amp100 from DH5alpha, and RP4 with Kan 50 from E. coli HB101).
 
===Cryo-Stocks===
===Cryo-Stocks===
:<strong>Krystle</strong>
:<strong>Krystle</strong>
:* Made cryo-stocks of pBLUSCRIPT, pUC18, and B0024.
:* Made cryo-stocks of pBLUSCRIPT, pUC18, and B0024.
 +
 +
===Run Gel for pRL1383a plasmid Prep===
 +
:<strong>Norman</strong>
 +
:*See experiment for details.
== Drylab Work ==
== Drylab Work ==
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= Quote of the Day =
= Quote of the Day =
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<blockquote>''History is the only laboratory we have in which to test the consequences of thought.'' - Étienne Gilson</blockquote>
+
<blockquote>''Note to self: if I have weird cancers in strange places, its probably because I was playing with these carcinogens.'' - KS</blockquote>
{{Team:Hawaii/Footer}}
{{Team:Hawaii/Footer}}
__NOTOC__
__NOTOC__

Latest revision as of 03:50, 26 June 2008

Projects Events Resources
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Notebook (t) Meetings (t)

Things we did today

Wetlab work

Alkaline Lysis Miniprep

Krystle & Margaret
  • Plasmid prep was performed according to the experimental protocol
  • Plasmid prep was performed with:
BioBricks
part number antibx media strength source organism
E0040 (GFP) amp100 DH5&alpha
J04430 (lac+GFP) DH5&alpha
B0034 (RBS) GP's super competent cells
B0024 (TT) DH5&alpha


Tool Plasmids
type antibx media strength source organism
pUC18 amp100 DH5&alpha
pBLUSCRIPT
RP1 kan50 E. coli

Cryo-Stocks

Krystle
  • Made cryo-stocks of pBLUSCRIPT, pUC18, and B0024.

Run Gel for pRL1383a plasmid Prep

Norman
  • See experiment for details.

Drylab Work

Primer Discussion

Krystle, Norman, & Margaret
  • Discussed the format of primers
  • worked on revising primers


Discussion

Quote of the Day

Note to self: if I have weird cancers in strange places, its probably because I was playing with these carcinogens. - KS


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