Team:The University of Alberta/Parts

From 2008.igem.org

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<br>
<br>
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<br>
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{| align="center"
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[[image:BioBrick.jpg]]
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|
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==University of Alberta Parts Registry==
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<h4>'''All our submitted parts can be found [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=University_of_Alberta in our section of the Parts Registry]!'''</h4> More detailed documentation can be found there for each part.
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==Plastic Project==
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[[Image:Plastic_Banner.jpg]]
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* TetR + LVA tail[http://partsregistry.org/Part:BBa_C0040|]
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* PLACIQ + ERE brick - [[Team:The_University_of_Alberta/Parts/Plastic/lacI_ERE_sequence|Sequence]]
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* Estrogen Receptor Alpha (Estrogen binding domain)
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* Estrogen Receptor Alpha (DNA binding domain)
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* BisdA BPA degredation bio brick - [[Team:The_University_of_Alberta/Parts/Plastic/BisDA_sequence|Sequence]]
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* BisdB BPA degredation bio brick - [[Team:The_University_of_Alberta/Parts/Plastic/BisDB_sequence|Sequence]]
==Parts Ordered==
==Parts Ordered==
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Sequences of synthetic genes ordered 20 Jun<br>
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PDF Sequences of synthetic genes ordered 20 Jun<br>
*[[Media:UA28-8genes-v2_(2).pdf]]
*[[Media:UA28-8genes-v2_(2).pdf]]
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Genbank files synthetic genes ordered 20 Jun<br>
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*[[Media:iGEM08_BisDA.gb]]
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*[[Media:iGEM08_BisDB.gb]]
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*[[Media:iGEM08_ER01.gb]]
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*[[Media:iGEM08_ER02.gb]]
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*[[Media:iGEM08_LacIQ_ERE.gb]]
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*[[Media:iGEM08_RBS_6XHis.gb]]
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*[[Media:iGEM08_TDNA_MCS.gb]]
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*[[Media:iGEM08_TetR_RBS_6XHis.gb]]
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==Plant Parts==
==Plant Parts==
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[[Image:Plant_Banner.jpg]]
[[Image:Plant_Banner.jpg]]
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<UL>
 
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<li>Plant CAMV 35S Promoter
 
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<li>Hygromycin Resistance Brick
 
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<li>IGEM Binary Vector
 
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<li>Blue OX, Purple Russian reporter systems}
 
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*Plant CAMV 35S Promoter
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*Plant Resistance Brick
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**Chloramphenicol was used
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*IGEM Binary Vector
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**We constructed the IGEM binary vector using P0380 as a starting point. We removed the PUC8 Multiple cloning site from this binary vector and supplemented our own which will allow the vector to accept biobricks.
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*Blue OX, Purple Russian reporter systems
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==Plastic Project==
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==Butanol Parts==
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{| align="center"
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[[Image:Plastic_Banner.jpg]]
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<h4> BioBricks Used This Summer</h4>
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* TetR + LVA tail
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* PLAC + ERE brick
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* [http://partsregistry.org/Part:BBa_I725021 RBS + B-hydroxy butyryl CoA dehydrogenase] (AKA. '''21''')
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* Estrogen Receptor Alpha (Estrogen binding domain)
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* [http://partsregistry.org/Part:BBa_I725022 RBS + Enoyl-CoA hydratase] (AKA. '''22''')
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* Estrogen Receptor Alpha (DNA binding domain)
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* [http://partsregistry.org/Part:BBa_I725023 RBS + Butyryl CoA dehydrogenase] (AKA. '''23''')
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* BisdA BPA degredation bio brick
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* [http://partsregistry.org/Part:BBa_I725024 RBS + Butyraldehyde dehydrogenase] (AKA. '''24''')
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* BisdB BPA degredation bio brick
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* [http://partsregistry.org/Part:BBa_I725025 RBS + Butanol dehydrogenase] (AKA. '''25''')
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* [http://partsregistry.org/Part:BBa_J61003 J61003] (without GFP)
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* [http://partsregistry.org/Part:BBa_I0500 I0500]
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* [http://partsregistry.org/Part:pSB1A3 pSB1A3]
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<h4>Parts Submitted</h4>
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* [http://partsregistry.org/Part:BBa_S04142 21 + 22]  (in plasmid pSB1A3)
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* [http://partsregistry.org/Part:BBa_S04145 21 + 22 + 23] (in plasmid pSB1A3)
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* [http://partsregistry.org/Part:BBa_S04143 23 + 24] (in plasmid pSB1A3)
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* [http://partsregistry.org/Part:BBa_S04144 24 + 25] (in plasmid pSB1A3)
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* [http://partsregistry.org/Part:BBa_S04147 21 + 22 + 23 + 24 + 25] (in plasmid pSB1A3)
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<h4>Parts Ordered This Summer</h4>
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*ETFA  (electron-transfer-flavoprotein, alpha polypeptide)
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*ETFB  (electron-transfer-flavoprotein, beta polypeptide)
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<h4>Parts Assembled This Summer</h4>
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<h5>For Protein Expression</h5>
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* J61003 + 21
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* J61003 + 22
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* J61003 + 23
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* J61003 + 24
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* J61003 + 25
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* I0500 + 21
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* I0500 + 22
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* I0500 + 23
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* I0500 + 24
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* I0500 + 25
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<h5>For Butanol Operon</h5>
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* 21 + 22  (in plasmid pSB1A3)
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* 21 + 22 + 23 (in plasmid pSB1A3)
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* 23 + 24 (in plasmid pSB1A3)
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* 24 + 25 (in plasmid pSB1A3)
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* 21 + 22 + 23 + 24 + 25 (in plasmid pSB1A3)
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* ETFA + ETFB (in plasmid Puc57)

Latest revision as of 01:35, 30 October 2008

[http://2008.igem.org/Team:The_University_of_Alberta HOMEUAB.png][http://2008.igem.org/Team:The_University_of_Alberta/Team TEAMUA.png][http://2008.igem.org/Team:The_University_of_Alberta/Project PROJECTUA.png][http://2008.igem.org/Team:The_University_of_Alberta/Parts PARTSUA.png][http://2008.igem.org/Team:The_University_of_Alberta/Notebook NOTEBOOKUA.png]

BioBrick.jpg

Contents

University of Alberta Parts Registry

All our submitted parts can be found [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=University_of_Alberta in our section of the Parts Registry]!

More detailed documentation can be found there for each part.

Plastic Project

Plastic Banner.jpg

  • TetR + LVA tail[http://partsregistry.org/Part:BBa_C0040|]
  • PLACIQ + ERE brick - Sequence
  • Estrogen Receptor Alpha (Estrogen binding domain)
  • Estrogen Receptor Alpha (DNA binding domain)
  • BisdA BPA degredation bio brick - Sequence
  • BisdB BPA degredation bio brick - Sequence

Parts Ordered

PDF Sequences of synthetic genes ordered 20 Jun

Genbank files synthetic genes ordered 20 Jun


Plant Parts

Plant Banner.jpg

  • Plant CAMV 35S Promoter
  • Plant Resistance Brick
    • Chloramphenicol was used
  • IGEM Binary Vector
    • We constructed the IGEM binary vector using P0380 as a starting point. We removed the PUC8 Multiple cloning site from this binary vector and supplemented our own which will allow the vector to accept biobricks.
  • Blue OX, Purple Russian reporter systems


Butanol Parts

BioBricks Used This Summer

  • [http://partsregistry.org/Part:BBa_I725021 RBS + B-hydroxy butyryl CoA dehydrogenase] (AKA. 21)
  • [http://partsregistry.org/Part:BBa_I725022 RBS + Enoyl-CoA hydratase] (AKA. 22)
  • [http://partsregistry.org/Part:BBa_I725023 RBS + Butyryl CoA dehydrogenase] (AKA. 23)
  • [http://partsregistry.org/Part:BBa_I725024 RBS + Butyraldehyde dehydrogenase] (AKA. 24)
  • [http://partsregistry.org/Part:BBa_I725025 RBS + Butanol dehydrogenase] (AKA. 25)
  • [http://partsregistry.org/Part:BBa_J61003 J61003] (without GFP)
  • [http://partsregistry.org/Part:BBa_I0500 I0500]
  • [http://partsregistry.org/Part:pSB1A3 pSB1A3]

Parts Submitted

  • [http://partsregistry.org/Part:BBa_S04142 21 + 22] (in plasmid pSB1A3)
  • [http://partsregistry.org/Part:BBa_S04145 21 + 22 + 23] (in plasmid pSB1A3)
  • [http://partsregistry.org/Part:BBa_S04143 23 + 24] (in plasmid pSB1A3)
  • [http://partsregistry.org/Part:BBa_S04144 24 + 25] (in plasmid pSB1A3)
  • [http://partsregistry.org/Part:BBa_S04147 21 + 22 + 23 + 24 + 25] (in plasmid pSB1A3)

Parts Ordered This Summer

  • ETFA (electron-transfer-flavoprotein, alpha polypeptide)
  • ETFB (electron-transfer-flavoprotein, beta polypeptide)

Parts Assembled This Summer

For Protein Expression
  • J61003 + 21
  • J61003 + 22
  • J61003 + 23
  • J61003 + 24
  • J61003 + 25
  • I0500 + 21
  • I0500 + 22
  • I0500 + 23
  • I0500 + 24
  • I0500 + 25
For Butanol Operon
  • 21 + 22 (in plasmid pSB1A3)
  • 21 + 22 + 23 (in plasmid pSB1A3)
  • 23 + 24 (in plasmid pSB1A3)
  • 24 + 25 (in plasmid pSB1A3)
  • 21 + 22 + 23 + 24 + 25 (in plasmid pSB1A3)
  • ETFA + ETFB (in plasmid Puc57)
Retrieved from "http://2008.igem.org/Team:The_University_of_Alberta/Parts"