Team:Bologna/Parts

From 2008.igem.org

(Difference between revisions)
(→Composites)
(→Composites)
 
(52 intermediate revisions not shown)
Line 1: Line 1:
<html>
<html>
<style type="text/css">
<style type="text/css">
-
 
+
 
body {background-color:#000000}
body {background-color:#000000}
strong {color: #c09a6d;}
strong {color: #c09a6d;}
Line 11: Line 11:
[[Image:Logo1a.gif|220px]][[Image:Testata_dx.jpg|745px]]
[[Image:Logo1a.gif|220px]][[Image:Testata_dx.jpg|745px]]
!align="center"|[[Team:Bologna|HOME]]
!align="center"|[[Team:Bologna|HOME]]
 +
!align="center"|[[Team:Bologna/Project|PROJECT]]
!align="center"|[[Team:Bologna/Team|TEAM]]
!align="center"|[[Team:Bologna/Team|TEAM]]
-
!align="center"|[[Team:Bologna/Project|PROJECT]]
+
!align="center"|[[Team:Bologna/Software|SOFTWARE]]
!align="center"|[[Team:Bologna/Modeling|MODELING]]
!align="center"|[[Team:Bologna/Modeling|MODELING]]
-
!align="center"|[[Team:Bologna/Notebook|WET-LAB]]
+
!align="center"|[[Team:Bologna/Wetlab|WET LAB]]
-
!align="center"|[[Team:Bologna/Software|SOFTWARE]]
+
!align="center"|[[Team:Bologna/Notebook|LAB-BOOK]]
!align="center"|[[Team:Bologna/Parts|SUBMITTED PARTS]]
!align="center"|[[Team:Bologna/Parts|SUBMITTED PARTS]]
!align="center"|[[Team:Bologna/Biosafety|BIOSAFETY AND PROTOCOLS]]
!align="center"|[[Team:Bologna/Biosafety|BIOSAFETY AND PROTOCOLS]]
-
 
|}
|}
Line 26: Line 26:
= Composites =
= Composites =
-
The table below shows all the composites that we have obtained to build up our final device. For each part you can find:  
+
The table below shows all the composites that were obtained and use to build up our final device. For each part, it ispossible to find:  
-
-in the second column the link to the Registry;  
+
-the link to the Registry in the second column ;  
-
-in the third column the components for each part;  
+
-part components, in the third column ;  
-in the fourth column the plasmid the part has been cloned in;  
-in the fourth column the plasmid the part has been cloned in;  
Line 43: Line 43:
{|  
{|  
|- style="background: #c09a6d; text-align: center;"
|- style="background: #c09a6d; text-align: center;"
-
|width=45| '''N°'''
+
|width=35| '''N°'''
-
|width=90| '''Igem Code'''
+
|width=85| '''Igem Code'''
-
|width=470| '''Fragment'''
+
|width=540| '''Fragment'''
|width=90| '''Plasmid'''
|width=90| '''Plasmid'''
-
|width=110| '''Length (bp)'''
+
|width=90| '''Length (bp)'''
-
|width=90| '''Vitality'''
+
|width=70| '''Vitality'''
-
|width=45| '''Fluo'''
+
|width=30| '''Fluo'''
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
| <center>1</center>
| <center>1</center>
-
| [http://partsregistry.org/Part:BBa_K079015 K0790015]   
+
| [http://partsregistry.org/Part:BBa_K079015 K079015]   
-
| LacY transporter protein from E. coli
+
| [[Team:Bologna/Project#Concluding_the_iGEM_2007_Project|LacY transporter protein from E. coli (for the improvement of the registry)]]
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1AK3 (HC)]
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1AK3 (HC)]
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079015 1254]
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079015 1254]
Line 60: Line 60:
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
| <center>2</center>
| <center>2</center>
-
| [http://partsregistry.org/Part:BBa_K079020 K079020]
 
-
| LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 2 operator
 
-
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)]
 
-
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079020 2165]
 
-
| <center>Yes</center>
 
-
| <center>-</center>
 
-
|- style="background:#f6efcd; color:black"
 
-
| <center>3</center>
 
-
| [http://partsregistry.org/Part:BBa_K079029 K079029]
 
-
| GFP expression controlled by LacI repressor under the BBa_J23114 promoter
 
-
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)]
 
-
| 2501
 
-
| <center>Yes</center>
 
-
| <center>-</center>
 
-
|- style="background:#f6efcd; color:black"
 
-
| <center>4</center>
 
| [http://partsregistry.org/Part:BBa_K079017 K079017]
| [http://partsregistry.org/Part:BBa_K079017 K079017]
| Lac symmetric - operator library member
| Lac symmetric - operator library member
Line 83: Line 67:
| <center>-</center>
| <center>-</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
-
| <center>5</center>
+
| <center>3</center>
| [http://partsregistry.org/Part:BBa_K079018 K079018]
| [http://partsregistry.org/Part:BBa_K079018 K079018]
| Lac 1 - operator library member
| Lac 1 - operator library member
Line 91: Line 75:
| <center>-</center>
| <center>-</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
-
| <center>6</center>
+
| <center>4</center>
| [http://partsregistry.org/Part:BBa_K079019 K079019]
| [http://partsregistry.org/Part:BBa_K079019 K079019]
| Lac 2 - operator library member
| Lac 2 - operator library member
Line 99: Line 83:
| <center>-</center>
| <center>-</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
-
| <center>7</center>
+
| <center>5</center>
| [http://partsregistry.org/Part:BBa_K079036 K079036]
| [http://partsregistry.org/Part:BBa_K079036 K079036]
| Tet O operator library member
| Tet O operator library member
Line 107: Line 91:
| <center>-</center>
| <center>-</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
-
| <center>8</center>
+
| <center>6</center>
| [http://partsregistry.org/Part:BBa_K079037 K079037]
| [http://partsregistry.org/Part:BBa_K079037 K079037]
| TetO-4C - operator library member
| TetO-4C - operator library member
Line 115: Line 99:
| <center>-</center>
| <center>-</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
-
| <center>9</center>
+
| <center>7</center>
| [http://partsregistry.org/Part:BBa_K079038 K079038]
| [http://partsregistry.org/Part:BBa_K079038 K079038]
| TetO-wt/4C5G - operator library member
| TetO-wt/4C5G - operator library member
Line 123: Line 107:
| <center>-</center>
| <center>-</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
-
| <center>10</center>
+
| <center>8</center>
| [http://partsregistry.org/Part:BBa_K079039 K079039]
| [http://partsregistry.org/Part:BBa_K079039 K079039]
| LexA 1 - operator library member
| LexA 1 - operator library member
Line 131: Line 115:
| <center>-</center>
| <center>-</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
-
| <center>11</center>
+
| <center>9</center>
| [http://partsregistry.org/Part:BBa_K079040 K079040]
| [http://partsregistry.org/Part:BBa_K079040 K079040]
| LexA 2 - opeartor library member
| LexA 2 - opeartor library member
Line 139: Line 123:
| <center>-</center>
| <center>-</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
-
| <center>12</center>
+
| <center>10</center>
| [http://partsregistry.org/Part:BBa_K079041 K079041]
| [http://partsregistry.org/Part:BBa_K079041 K079041]
| Lambda OR1 - operator library member
| Lambda OR1 - operator library member
Line 147: Line 131:
| <center>-</center>
| <center>-</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
-
| <center>13</center>
+
| <center>11</center>
| [http://partsregistry.org/Part:BBa_K079042 K079042]
| [http://partsregistry.org/Part:BBa_K079042 K079042]
| Lambda OR2 - operator library member
| Lambda OR2 - operator library member
Line 155: Line 139:
| <center>-</center>
| <center>-</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
-
| <center>14</center>
+
| <center>12</center>
| [http://partsregistry.org/Part:BBa_K079043 K079043]
| [http://partsregistry.org/Part:BBa_K079043 K079043]
| Lambda OR3 - operator library member
| Lambda OR3 - operator library member
Line 163: Line 147:
| <center>-</center>
| <center>-</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
-
| <center>15</center>
+
| <center>13</center>
| [http://partsregistry.org/Part:BBa_K079045 K079045]
| [http://partsregistry.org/Part:BBa_K079045 K079045]
| Lac operator library
| Lac operator library
Line 171: Line 155:
| <center>-</center>
| <center>-</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
-
| <center>16</center>
+
| <center>14</center>
| [http://partsregistry.org/Part:BBa_K079046 K079046]
| [http://partsregistry.org/Part:BBa_K079046 K079046]
| Tet operator library
| Tet operator library
Line 179: Line 163:
| <center>-</center>
| <center>-</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
-
| <center>17</center>
+
| <center>15</center>
| [http://partsregistry.org/Part:BBa_K079047 K079047]
| [http://partsregistry.org/Part:BBa_K079047 K079047]
| Lambda operator library
| Lambda operator library
Line 187: Line 171:
| <center>-</center>
| <center>-</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
-
| <center>18</center>
+
| <center>16</center>
| [http://partsregistry.org/Part:BBa_K079048 K079048]
| [http://partsregistry.org/Part:BBa_K079048 K079048]
| LexA operator library
| LexA operator library
Line 194: Line 178:
| <center>Yes</center>
| <center>Yes</center>
| <center>-</center>
| <center>-</center>
 +
|- style="background:#f6efcd; color:black"
 +
| <center>17</center>
 +
| [http://partsregistry.org/Part:BBa_K079020 K079020]
 +
| GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein
 +
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)]
 +
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079020 2165]
 +
| <center>Yes</center>
 +
| <center>[[Team:Bologna/Parts/FluoImages#K079020|►]]</center>
 +
|- style="background:#f6efcd; color:black"
 +
| <center>18</center>
 +
| [http://partsregistry.org/Part:BBa_K079026 K079026]
 +
| GFP reporter protein and LacI gene sequence under the control of J23118 constitutive promoter
 +
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)]
 +
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079026 2116]
 +
| <center>Yes</center>
 +
| <center>[[Team:Bologna/Parts/FluoImages#K079026|►]]</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
| <center>19</center>
| <center>19</center>
 +
| [http://partsregistry.org/Part:BBa_K079029 K079029]
 +
| GFP expression controlled by LacI repressor under the BBa_J23114 promoter
 +
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)]
 +
| 2501
 +
| <center>Yes</center>
 +
| <center>[[Team:Bologna/Parts/FluoImages#K079029|►]]</center>
 +
|- style="background:#f6efcd; color:black"
 +
| <center>20</center>
| [http://partsregistry.org/Part:BBa_K079049 K079049]
| [http://partsregistry.org/Part:BBa_K079049 K079049]
| GFP reporter protein under the control of the J23118 constitutive promoter and LexA 2 operator
| GFP reporter protein under the control of the J23118 constitutive promoter and LexA 2 operator
Line 203: Line 211:
| <center>-</center>
| <center>-</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
-
| <center>20</center>
+
| <center>21</center>
| [http://partsregistry.org/Part:BBa_K079050 K079050]
| [http://partsregistry.org/Part:BBa_K079050 K079050]
| GFP reporter protein under the control of the J23100 constitutive promoter and LexA 2 operator
| GFP reporter protein under the control of the J23100 constitutive promoter and LexA 2 operator
Line 209: Line 217:
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079050 981]
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079050 981]
| <center>Yes</center>
| <center>Yes</center>
-
| <center>-</center>
+
| <center>[[Team:Bologna/Parts/FluoImages#K079050|►]]</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
-
| <center>21</center>
+
| <center>22</center>
| [http://partsregistry.org/Part:BBa_K079023 K079023]
| [http://partsregistry.org/Part:BBa_K079023 K079023]
-
| LacI repressor under the control of the J23118 constitutive promoter and Lac 2 operator
+
| Auto-regulated J23118 constitutive promoter and Lac 2 operator  
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)]
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)]
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079023 1243]
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079023 1243]
Line 219: Line 227:
| <center>-</center>
| <center>-</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
-
| <center>22</center>
+
| <center>23</center>
-
| [http://partsregistry.org/Part:BBa_K079023 K079024]
+
| [http://partsregistry.org/Part:BBa_K079024 K079024]
| LacI repressor under the control of the J23118 constitutive promoter and Lac 1 operator
| LacI repressor under the control of the J23118 constitutive promoter and Lac 1 operator
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)]
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)]
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079024 1243]
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079024 1243]
-
| <center>Yes</center>
 
-
| <center>-</center>
 
-
|- style="background:#f6efcd; color:black"
 
-
| <center>23</center>
 
-
| [http://partsregistry.org/Part:BBa_K079026 K079026]
 
-
| GFP reporter protein regulated by J23118 constitutive promoter
 
-
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)]
 
-
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079026 2116]
 
| <center>Yes</center>
| <center>Yes</center>
| <center>-</center>
| <center>-</center>
Line 237: Line 237:
| <center>24</center>
| <center>24</center>
| [http://partsregistry.org/Part:BBa_K079030 K079030]  
| [http://partsregistry.org/Part:BBa_K079030 K079030]  
-
| GFP reporter protein controlled by the BBa_23105 constitutive promoter
+
| GFP reporter protein under the control of the BBa_23105 constitutive promoter  
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)]
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)]
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079030 957]
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079030 957]
| <center>Yes</center>
| <center>Yes</center>
-
| <center>-</center>
+
| <center>[[Team:Bologna/Parts/FluoImages#K079030|►]]</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
| <center>25</center>
| <center>25</center>
Line 249: Line 249:
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079031 957]
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079031 957]
| <center>Yes</center>
| <center>Yes</center>
-
| <center>-</center>
+
| <center>[[Team:Bologna/Parts/FluoImages#K079031|►]]</center>
|- style="background:#f6efcd; color:black"
|- style="background:#f6efcd; color:black"
| <center>26</center>
| <center>26</center>
Line 257: Line 257:
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079032 957]
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079032 957]
| <center>Yes</center>
| <center>Yes</center>
-
| <center>-</center>
+
| <center>[[Team:Bologna/Parts/FluoImages#K079032|►]]</center>
|}
|}
-
 
-
<ref name= NomeNota > TestoNota </ref>
 
-
==Note==
 
-
<ref name= NomeNota />
 
-
    <ref name= Nome_Nota />
 
-
    <ref name= "Nome Nota" />
 
----
----
-
[https://2008.igem.org/Team:Bologna ''Up'']
+
[https://2008.igem.org/Team:Bologna/Parts ''Up'']

Latest revision as of 02:18, 30 October 2008

Logo1a.gifTestata dx.jpg
HOME PROJECT TEAM SOFTWARE MODELING WET LAB LAB-BOOK SUBMITTED PARTS BIOSAFETY AND PROTOCOLS


Composites

The table below shows all the composites that were obtained and use to build up our final device. For each part, it ispossible to find:

-the link to the Registry in the second column ;

-part components, in the third column ;

-in the fourth column the plasmid the part has been cloned in;

-in the fifth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;

-in the sixth column cell vitality after transformation;

-in the seventh column fluo data for each part.


N° Igem Code Fragment Plasmid Length (bp) Vitality Fluo
1
 [http://partsregistry.org/Part:BBa_K079015 K079015] LacY transporter protein from E. coli (for the improvement of the registry) [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1AK3 (HC)] 1254
Yes
-
2
 [http://partsregistry.org/Part:BBa_K079017 K079017] Lac symmetric - operator library member GENEART pGA18 20
Yes
-
3
 [http://partsregistry.org/Part:BBa_K079018 K079018] Lac 1 - operator library member GENEART pGA18 21
Yes
-
4
 [http://partsregistry.org/Part:BBa_K079019 K079019] Lac 2 - operator library member GENEART pGA18 21
Yes
-
5
 [http://partsregistry.org/Part:BBa_K079036 K079036] Tet O operator library member GENEART pMA 15
Yes
-
6
 [http://partsregistry.org/Part:BBa_K079037 K079037] TetO-4C - operator library member GENEART pMA 15
Yes
-
7
 [http://partsregistry.org/Part:BBa_K079038 K079038] TetO-wt/4C5G - operator library member GENEART pMA 15
Yes
-
8
 [http://partsregistry.org/Part:BBa_K079039 K079039] LexA 1 - operator library member GENEART pMA 16
Yes
-
9
 [http://partsregistry.org/Part:BBa_K079040 K079040] LexA 2 - opeartor library member GENEART pMA 16
Yes
-
10
 [http://partsregistry.org/Part:BBa_K079041 K079041] Lambda OR1 - operator library member GENEART pMA 17
Yes
-
11
 [http://partsregistry.org/Part:BBa_K079042 K079042] Lambda OR2 - operator library member GENEART pMA 17
Yes
-
12
 [http://partsregistry.org/Part:BBa_K079043 K079043] Lambda OR3 - operator library member GENEART pMA 17
Yes
-
13
 [http://partsregistry.org/Part:BBa_K079045 K079045] Lac operator library GENEART pGA18 78
Yes
-
14
 [http://partsregistry.org/Part:BBa_K079046 K079046] Tet operator library GENEART pMA 61
Yes
-
15
 [http://partsregistry.org/Part:BBa_K079047 K079047] Lambda operator library GENEART pMA 67
Yes
-
16
 [http://partsregistry.org/Part:BBa_K079048 K079048] LexA operator library GENEART pMA 40
Yes
-
17
 [http://partsregistry.org/Part:BBa_K079020 K079020] GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 2165
Yes
►
18
 [http://partsregistry.org/Part:BBa_K079026 K079026] GFP reporter protein and LacI gene sequence under the control of J23118 constitutive promoter [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 2116
Yes
►
19
 [http://partsregistry.org/Part:BBa_K079029 K079029] GFP expression controlled by LacI repressor under the BBa_J23114 promoter [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 2501
Yes
►
20
 [http://partsregistry.org/Part:BBa_K079049 K079049] GFP reporter protein under the control of the J23118 constitutive promoter and LexA 2 operator [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 981
Yes
-
21
 [http://partsregistry.org/Part:BBa_K079050 K079050] GFP reporter protein under the control of the J23100 constitutive promoter and LexA 2 operator [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 981
Yes
►
22
 [http://partsregistry.org/Part:BBa_K079023 K079023] Auto-regulated J23118 constitutive promoter and Lac 2 operator [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 1243
Yes
-
23
 [http://partsregistry.org/Part:BBa_K079024 K079024] LacI repressor under the control of the J23118 constitutive promoter and Lac 1 operator [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 1243
Yes
-
24
 [http://partsregistry.org/Part:BBa_K079030 K079030] GFP reporter protein under the control of the BBa_23105 constitutive promoter [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 957
Yes
►
25
 [http://partsregistry.org/Part:BBa_K079031 K079031] GFP reporter protein under the control of the BBa_J23118 promoter [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 957
Yes
►
26
 [http://partsregistry.org/Part:BBa_K079032 K079032] GFP reporter protein under the control of the BBa_J23100 constitutive promoter [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 957
Yes
►


Up
Retrieved from "http://2008.igem.org/Team:Bologna/Parts"