Team:NTU-Singapore/Notebook/23 June 2008

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=Monday 23 June=
=Monday 23 June=
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{|border="1" style="background-color:#ffffcc;" cellpadding="20"
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==Morning (9am-1230pm)==
==Morning (9am-1230pm)==
*DNA extraction (using Miniprep kit) for 21 samples that Choon Kit inoculated on [https://2008.igem.org/wiki/index.php?title=Team:NTU-Singapore/Notebook/22_June_2008 Sunday]
*DNA extraction (using Miniprep kit) for 21 samples that Choon Kit inoculated on [https://2008.igem.org/wiki/index.php?title=Team:NTU-Singapore/Notebook/22_June_2008 Sunday]
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*Digestion LacI promoter insert using SpeI and EcoRI sequentially with QiAgen PCR purification kit in between.
*Digestion LacI promoter insert using SpeI and EcoRI sequentially with QiAgen PCR purification kit in between.
*Digestion of RBS 0034 vector using XbaI and EcoRI sequentially and QIAgen PCR purification kit.
*Digestion of RBS 0034 vector using XbaI and EcoRI sequentially and QIAgen PCR purification kit.
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===Chin Chong===
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**Preapre LBA agar plates of Top10 and BL21 containing LacI-GFP and allowed to incubate overnight at 37 deg cel
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**Designed the Lysis gene (with Promoter and RBS) and LsrA gene and requested for Quotations from companies
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**Inoculate top10 and BL21 cells containing LacI-GFP and grow in two seperate tubes containing 5 ml of LBA 
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***allowed to incubate overnight at 37 deg cel
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**Autoclaved MgSO4, CaCl2 and M9 salts
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Latest revision as of 02:00, 28 October 2008

Monday 23 June

Morning (9am-1230pm)

  • DNA extraction (using Miniprep kit) for 21 samples that Choon Kit inoculated on Sunday
  • Digestion of the above 21 plasmids by EcoRI and PstI, followed by Gel electrophoresis check after 2 hours incubation.
  • Digestion LacI promoter insert using SpeI and EcoRI sequentially with QiAgen PCR purification kit in between.
  • Digestion of RBS 0034 vector using XbaI and EcoRI sequentially and QIAgen PCR purification kit.


Chin Chong

    • Preapre LBA agar plates of Top10 and BL21 containing LacI-GFP and allowed to incubate overnight at 37 deg cel
    • Designed the Lysis gene (with Promoter and RBS) and LsrA gene and requested for Quotations from companies
    • Inoculate top10 and BL21 cells containing LacI-GFP and grow in two seperate tubes containing 5 ml of LBA
      • allowed to incubate overnight at 37 deg cel
    • Autoclaved MgSO4, CaCl2 and M9 salts
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