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Team:Warsaw/Calendar-Main/26 June 2008
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<h4>Piotr, Weronika</h4> | <h4>Piotr, Weronika</h4> | ||
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| - | <li>Isolation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC30</a></li> | + | <li>Isolation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC30</a>.</li> |
| - | <li>Inoculation of E.coli TOP10 carrying <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A2>pSB1A2</a> standard plasmids carrying parts: <A href=http://partsregistry.org/wiki/index.php?title=Part:BBa_E0840>BBa_E0840</a>(GFP genetrator) and <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450>BBa_J04450</a> (RFP generator) | + | <li>Inoculation of E.coli TOP10 carrying <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A2>pSB1A2</a> standard plasmids carrying parts: <A href=http://partsregistry.org/wiki/index.php?title=Part:BBa_E0840>BBa_E0840</a>(GFP genetrator) and <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450>BBa_J04450</a> (RFP generator). |
</li></ol> | </li></ol> | ||
Latest revision as of 14:02, 28 October 2008
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Change of the reporter from pZC320 with B-galactosidase to GFP or RFPPiotr, Weronika
Preparation of constructs: OmpA_alpha and OmpA_omegaMichał K.Repetition of PCRs, gel-out isolations and digests from 17 June. Preparation of alpha-A and omega-A fusionsMichał L., Ewa, MarcinPolymerase Chain Ligation (PCL)
PCL program for fusion A with alpha and omega
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