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Team:Chiba/Calendar-Home/11 October 2008
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[[Team:Chiba/Calendar-Home/10 October 2008|10 October 2008 <]]|[[Team:Chiba/Calendar-Home/12 October 2008|> 12 October 2008]] | [[Team:Chiba/Calendar-Home/10 October 2008|10 October 2008 <]]|[[Team:Chiba/Calendar-Home/12 October 2008|> 12 October 2008]] | ||
| - | + | ==Laboratory work== | |
| - | + | ===Team:Demo-Rs=== | |
| - | + | ~10 October | |
| - | + | #Sender Wash | |
| - | + | ##Centrifuged 2 tubes containing([http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908))at 20°C,3600rpm for 6min and discarded supernatant. | |
| - | + | ##Added 10mL LB-Amp to each tube. | |
| - | + | ##Repeated wash twice. | |
| - | + | #Creating bacterial plates | |
| - | + | ##LB-Amp pre-cultured Sender([http://partsregistry.org/Part:BBa_S03623 BBa_S03623](JW1908)) tube(10mL) was mixed with LB-Amp-agar(50°C)(10ml)to produce sender containing bacterialplate. | |
| + | #Lifted with nitrocellulose | ||
| + | ##Receiver([http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908))colony was transfered to a nitrocellulose filter and placed on each of Sender([http://partsregistry.org/Part:BBa_S03623 BBa_S03623](JW1908))containing bacterial plate and Sender-absent negative control plate (t=0). | ||
| + | #Method to detect fluorescence | ||
| + | ##Plates cultured at 37°C were exposed to UV (312nm) light once every 30 minutes to observe GFP fluorescence. | ||
Latest revision as of 00:38, 30 October 2008
10 October 2008 <|> 12 October 2008
Laboratory work
Team:Demo-Rs
~10 October
- Sender Wash
- Centrifuged 2 tubes containing([http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908))at 20°C,3600rpm for 6min and discarded supernatant.
- Added 10mL LB-Amp to each tube.
- Repeated wash twice.
- Creating bacterial plates
- LB-Amp pre-cultured Sender([http://partsregistry.org/Part:BBa_S03623 BBa_S03623](JW1908)) tube(10mL) was mixed with LB-Amp-agar(50°C)(10ml)to produce sender containing bacterialplate.
- Lifted with nitrocellulose
- Receiver([http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908))colony was transfered to a nitrocellulose filter and placed on each of Sender([http://partsregistry.org/Part:BBa_S03623 BBa_S03623](JW1908))containing bacterial plate and Sender-absent negative control plate (t=0).
- Method to detect fluorescence
- Plates cultured at 37°C were exposed to UV (312nm) light once every 30 minutes to observe GFP fluorescence.
