"
EPF-Lausanne/20 October 2008
From 2008.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
[https://2008.igem.org/wiki/index.php?title=EPF-Lausanne/13_October_2008 <<Previous] - [https://2008.igem.org/Team:EPF-Lausanne/Notebook Back to Notebook] - | [https://2008.igem.org/wiki/index.php?title=EPF-Lausanne/13_October_2008 <<Previous] - [https://2008.igem.org/Team:EPF-Lausanne/Notebook Back to Notebook] - | ||
| - | [https://2008.igem.org/wiki/index.php?title=EPF-Lausanne/ | + | [https://2008.igem.org/wiki/index.php?title=EPF-Lausanne/22_October_2008 Next>>] |
==Cloning== | ==Cloning== | ||
Latest revision as of 09:05, 29 October 2008
<<Previous - Back to Notebook - Next>>
Cloning
- Cloning of BC1 into AB
- Using the simplified ligation protocol.
- Cloning of CD into LuxI+ (LuxI with RBS and Term.)
- Here a modified version of the ligation protocol was used, because the insert is quite big (over 2kb).
- Reaction mix:
- 3μl vector
- 5.5μl insert
- 1μl Ligase Buffer with ATP
- 0.5μl T4 Ligase
=> Cloning worked.
