"
Team:Heidelberg/Notebook/Killing I/Cloning
From 2008.igem.org
(Difference between revisions)
(→Killing I - Cloning strategy) |
(→Killing I - Cloning strategy) |
||
| (6 intermediate revisions not shown) | |||
| Line 464: | Line 464: | ||
==Killing I - Cloning strategy== | ==Killing I - Cloning strategy== | ||
| - | |||
| - | |||
| + | Our goal to clone a modified bacteriophage λ which can be transported by conjugation, allows the selection of infected cells through a chloamphenicol resistance and harbours GFP for visualization was designed in two strategies whereas strategy two builds on strategy one. A detailed description of the two strategies can be seen below (see 1.1 and 1.2). The used λ cI protein generator was synthesised by geneart and just transferred in a standard plasmid as it can be seen in 1.3. | ||
| - | [[Image:HD_phage2_klein.png]] | + | |
| + | === phage cloning strategy one=== | ||
| + | [[Image:HD_phage1_klein.gif|900px]] | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | === phage cloning strategy two=== | ||
| + | [[Image:HD_phage2_klein.png|900px]] | ||
| + | |||
| + | |||
| + | |||
| + | === Bacteriophage λ cI cloning strategy=== | ||
| + | [[Image:HD_phage3.png|200px]] | ||
Latest revision as of 09:08, 29 October 2008
Contents |
Killing I - Cloning strategy
Our goal to clone a modified bacteriophage λ which can be transported by conjugation, allows the selection of infected cells through a chloamphenicol resistance and harbours GFP for visualization was designed in two strategies whereas strategy two builds on strategy one. A detailed description of the two strategies can be seen below (see 1.1 and 1.2). The used λ cI protein generator was synthesised by geneart and just transferred in a standard plasmid as it can be seen in 1.3.
phage cloning strategy one
phage cloning strategy two
Bacteriophage λ cI cloning strategy
