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Team:The University of Alberta/30 September 2008
From 2008.igem.org
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(New page: *Tom can't tell much from the gel2 gel1. <br>) |
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*Tom | *Tom | ||
| - | can't tell much from the gel2 | + | can't tell much from the gel2<br> |
| - | gel1. <br> | + | gel1. expected size 3000bp (2-9) and 2000bp(10-16). this is as expected <br> |
| + | gel2. expected size 4000bp (1-8) and 4200bp(10-16). result is as expected, but have some short band(contaminations?)<br> | ||
| + | they all seems good! have to sequence to make sure. | ||
| + | |||
| + | |||
| + | ==David== | ||
| + | |||
| + | starting the bisdA and bisdB cassettes from scratch because i really, really, really want to see if this stuff will work. | ||
| + | |||
| + | i digested | ||
| + | |||
| + | the correct pTET vector complete with RBS site and 6xHis tag (pTET) with SphI+PstI+CIP treatment | ||
| + | BisdA with XbaI+PstI | ||
| + | BisdB with XbaI+PstI | ||
| + | |||
| + | i gel purified the products and set up the following ligations | ||
| + | |||
| + | pTET+BisdA | ||
| + | pTET+BisdB | ||
Latest revision as of 03:34, 29 October 2008
- Tom
can't tell much from the gel2
gel1. expected size 3000bp (2-9) and 2000bp(10-16). this is as expected
gel2. expected size 4000bp (1-8) and 4200bp(10-16). result is as expected, but have some short band(contaminations?)
they all seems good! have to sequence to make sure.
David
starting the bisdA and bisdB cassettes from scratch because i really, really, really want to see if this stuff will work.
i digested
the correct pTET vector complete with RBS site and 6xHis tag (pTET) with SphI+PstI+CIP treatment BisdA with XbaI+PstI BisdB with XbaI+PstI
i gel purified the products and set up the following ligations
pTET+BisdA pTET+BisdB
