Team:The University of Alberta/2 October 2008
From 2008.igem.org
(Difference between revisions)
(New page: *Tom ** ETFA/B already have RBS site, they are ready for use ** setup overnight of 21-25(1-4); ETFA/B (5-6) ** redo 21-25 ligation tomorrow) |
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** setup overnight of 21-25(1-4); ETFA/B (5-6) | ** setup overnight of 21-25(1-4); ETFA/B (5-6) | ||
** redo 21-25 ligation tomorrow | ** redo 21-25 ligation tomorrow | ||
+ | ** religate 21+22+23(S/P) AND 24+25(X/P) | ||
+ | ** sequence ETFA/B with Vf and Vr | ||
+ | |||
+ | ==David== | ||
+ | |||
+ | i picked candidate colonies from yesterdays transformations to grow up overnight in LB amp. | ||
+ | |||
+ | pTET+BisdA | ||
+ | pTET+BisdB |
Latest revision as of 03:37, 29 October 2008
- Tom
- ETFA/B already have RBS site, they are ready for use
- setup overnight of 21-25(1-4); ETFA/B (5-6)
- redo 21-25 ligation tomorrow
- religate 21+22+23(S/P) AND 24+25(X/P)
- sequence ETFA/B with Vf and Vr
David
i picked candidate colonies from yesterdays transformations to grow up overnight in LB amp.
pTET+BisdA pTET+BisdB