"
Team:The University of Alberta/9 October 2008
From 2008.igem.org
(Difference between revisions)
(New page: *Tom ** run the colony PCR on gel *** get size: 21-25 5.5Kb, ETFA 2kb and 2.8kb *** digest PCR with X/P) |
|||
| Line 3: | Line 3: | ||
*** get size: 21-25 5.5Kb, ETFA 2kb and 2.8kb | *** get size: 21-25 5.5Kb, ETFA 2kb and 2.8kb | ||
*** digest PCR with X/P | *** digest PCR with X/P | ||
| + | |||
| + | ==David== | ||
| + | i miniprepped yesterdays cultures and checked them with NotI. they worked. we now have pUC57-pTET-BisdA+pTET-BisdB. | ||
Latest revision as of 03:46, 29 October 2008
- Tom
- run the colony PCR on gel
- get size: 21-25 5.5Kb, ETFA 2kb and 2.8kb
- digest PCR with X/P
- run the colony PCR on gel
David
i miniprepped yesterdays cultures and checked them with NotI. they worked. we now have pUC57-pTET-BisdA+pTET-BisdB.
