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Wiki/Team:Warsaw/igem notebook.htm
From 2008.igem.org
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| - | < | + | <td align=center width=70%><h1><p style="font-size: 16px; text-align: center; text-decoration: bold;">Table of contents </p></h1></td><td> </td></tr> |
<td width=70% valign=top style="border-left-style: grooved"> | <td width=70% valign=top style="border-left-style: grooved"> | ||
| - | <h3 | + | <h3 onClick=switchMenu('one-armed-vect') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';"> Preparation of one-armed bandit vectors</h3> |
| - | <div name="menu_item" style="display: none" id="one-armed-vect" > | + | <div name="menu_item" style="display: none" id="one-armed-vect"> |
<ol> | <ol> | ||
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| - | <h3 | + | <h3 OnClick=switchMenu('one-armed-tests') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';"> One-armed bandit tests</h3> |
<div name="menu_item" style="display: none" id="one-armed-tests"> | <div name="menu_item" style="display: none" id="one-armed-tests"> | ||
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</div> | </div> | ||
| - | <h3 | + | <h3 OnClick=switchMenu('hunter-vect') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Preparation of vectors for 'hunter' part of 'hunter and prey'</h3> |
<div name="menu_item" id="hunter-vect" style="display: none"> | <div name="menu_item" id="hunter-vect" style="display: none"> | ||
<ol> | <ol> | ||
| - | <li class="nb_toc1" | + | <li class="nb_toc1" OnClick=switchMenu('prot_A','submenu_item')>Preparation of vectors with protein A derivatives fusions</li> |
<div name="submenu_item" id="prot_A" style="display: none"> | <div name="submenu_item" id="prot_A" style="display: none"> | ||
<ul> | <ul> | ||
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=7_July_2008&arg1=8_July_2008&arg2=14_July_2008&arg3=15_July_2008&arg4=16_July_2008&arg5=21_July_2008&arg6=22_July_2008&arg7=23_July_2008&arg8=28_July_2008&name=Cloning%20omega-A%20fusion%20on%20pKS%20%28second%20attempt%29>Cloning omega-A fusion on pKS (second attempt)</a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=7_July_2008&arg1=8_July_2008&arg2=14_July_2008&arg3=15_July_2008&arg4=16_July_2008&arg5=21_July_2008&arg6=22_July_2008&arg7=23_July_2008&arg8=28_July_2008&name=Cloning%20omega-A%20fusion%20on%20pKS%20%28second%20attempt%29>Cloning omega-A fusion on pKS (second attempt)</a></li> | ||
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Preparation%20of%20construct%20pKS%20with%20A%20protein>Preparation of construct pKS with A protein </a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Preparation%20of%20construct%20pKS%20with%20A%20protein>Preparation of construct pKS with A protein </a></li> | ||
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=21_July_2008&arg1=22_July_2008&arg2=23_July_2008&arg3=24_July_2008&arg4=25_July_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20OmpA%20constructs>Cloning of protein A DNA to OmpA constructs </a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=21_July_2008&arg1=22_July_2008&arg2=23_July_2008&arg3=24_July_2008&arg4=25_July_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20OmpA%20constructs>Cloning of protein A DNA to OmpA constructs </a></li> | ||
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=18_August_2008&arg1=19_August_2008&arg2=20_August_2008&arg3=21_August_2008&arg4=22_August_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20GeneArt%20plasmid">Cloning of protein A DNA to GeneArt plasmid</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=18_August_2008&arg1=19_August_2008&arg2=20_August_2008&arg3=21_August_2008&arg4=22_August_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20GeneArt%20plasmid">Cloning of protein A DNA to GeneArt plasmid</a></li> | ||
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=22_September_2008&arg1=23_September_2008&arg2=24_September_2008&arg3=25_September_2008&arg4=26_September_2008&arg5=27_September_2008&arg6=30_September_2008&name=Mutagenesis%20of%20protein%20A">Mutagenesis of protein A</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=22_September_2008&arg1=23_September_2008&arg2=24_September_2008&arg3=25_September_2008&arg4=26_September_2008&arg5=27_September_2008&arg6=30_September_2008&name=Mutagenesis%20of%20protein%20A">Mutagenesis of protein A</a></li> | ||
| - | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=24_September_2008&arg1=25_September_2008&name=Preparation%20of%20alpha_A%20construct">Preparation of alpha_A construct</a></li> | + | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=14_July_2008&arg1=15_July_2008&name=Preparation%20of%20alpha%20A%20conctruct>Preparation of alpha A construct #1</a></li> |
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| + | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=24_September_2008&arg1=25_September_2008&name=Preparation%20of%20alpha_A%20construct">Preparation of alpha_A construct #2</a></li> | ||
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</ul> | </ul> | ||
</div> | </div> | ||
| - | <li class="nb_toc1" | + | <li class="nb_toc1" OnClick=switchMenu('delta_A','submenu_item')>Preparation of vectors with B-domain of protein A derivatives fusions</li> |
| - | <div name="submenu_item" id=" | + | <div name="submenu_item" id="delta_A" style="display: none"> |
<ul> | <ul> | ||
| + | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=30_July_2008&arg1=31_July_2008&arg2=1_August_2008&arg3=4_August_2008&arg4=5_August_2008&name=Cloning%20of%20truncated%20fragment%20of%20protein%20A%20%28%26Delta%3BA%29>Cloning of truncated fragment of protein A (ΔA) </a></li> | ||
| - | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num= | + | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=29_July_2008&arg1=30_July_2008&arg2=31_July_2008&name=Cloning%20of%20omega_%26Delta%3BA%20DNA%20fragment%20to%20pACYC177%20OmpA_alpha>Cloning of omega_ΔA DNA fragment to pACYC177 OmpA_alpha </a></li> |
| - | <li class="nb_toc"><a href= | + | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&arg3=7_August_2008&name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct>Preparing pACYC177 OmpA_omega_ΔA construct </a></li> |
| - | </ul></div> | + | </ul> |
| + | </div> | ||
| + | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=14_July_2008&arg1=15_July_2008&arg2=16_July_2008&arg3=17_July_2008&arg4=18_July_2008&arg5=19_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20OmpA%20constructs>Cloning of protein Z DNA to OmpA constructs </a></li> | ||
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| + | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=13_August_2008&arg1=14_August_2008&arg2=18_August_2008&arg3=19_August_2008&arg4=20_August_2008&arg5=21_August_2008&arg6=22_August_2008&arg7=25_August_2008&arg8=26_August_2008&name=Cloning%20of%20alpha%20to%20pACYC177%20OmpA_A_omega%20and%20pACYC177%20OmpA_Z_omega">Cloning of alpha to pACYC177 OmpA_A_omega and pACYC177 OmpA_Z_omega</a></li> | ||
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</div> | </div> | ||
| - | <h3 | + | <h3 OnClick=switchMenu('prey-vect') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Preparation of vectors for 'prey' part of 'hunter and prey'</h3> |
<div name="menu_item" id="prey-vect" style="display: none"> | <div name="menu_item" id="prey-vect" style="display: none"> | ||
<ol> | <ol> | ||
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=17_July_2008&arg1=18_July_2008&arg2=21_July_2008&arg3=22_July_2008&arg4=23_July_2008&arg5=24_July_2008&arg6=25_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-omega%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA </a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=17_July_2008&arg1=18_July_2008&arg2=21_July_2008&arg3=22_July_2008&arg4=23_July_2008&arg5=24_July_2008&arg6=25_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-omega%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA </a></li> | ||
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-alpha%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpA </a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-alpha%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpA </a></li> | ||
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</div> | </div> | ||
| - | <h3 | + | <h3 OnClick=switchMenu('prey-purif') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Purification of prey proteins</h3> |
<div name="menu_item" id="prey-purif" style="display: none"> | <div name="menu_item" id="prey-purif" style="display: none"> | ||
<ol> | <ol> | ||
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega>Purification of proteins: Z-alpha and Z-omega </a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega>Purification of proteins: Z-alpha and Z-omega </a></li> | ||
| - | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=8&arg0=29_August_2008& | + | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=8&arg0=29_August_2008&arg1=1_September_2008&arg2=2_September_2008&arg3=3_September_2008&arg4=4_September_2008&arg5=5_September_2008&arg6=8_September_2008&arg7=9_September_2008&name=Purification%20of%20proteins%3A%20A-alpha">Purification of proteins: A-alpha</a></li> |
| - | amp;arg1=1_September_2008&arg2=2_September_2008&arg3=3_September_2008&arg4=4_September_2008&arg5=5_September_2008&arg6=8_September_2008&arg7=9_September_2008&name=Purification%20of%20proteins%3A%20A-alpha">Purification of proteins: A-alpha</a></li> | + | |
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| - | <h3 | + | <h3 OnClick="switchMenu('hunter-tests')" onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">'Hunter and prey' tests</h3> |
<div name="menu_item" id="hunter-tests" style="display: none"> | <div name="menu_item" id="hunter-tests" style="display: none"> | ||
<ol> | <ol> | ||
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| - | <li class="nb_toc1" | + | <li class="nb_toc1" OnClick="switchMenu('resist-tests','submenu_item')">Ampicillin-resistance tests</li> |
<div name="submenu_item" id="resist-tests" style="display: none"> | <div name="submenu_item" id="resist-tests" style="display: none"> | ||
<ul> | <ul> | ||
| - | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&name=Checking%20if%20OmpA_omega_%26Delta%3BA_alpha%20gives%20ampicillin%20resistance>Checking if | + | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&name=Checking%20if%20OmpA_omega_%26Delta%3BA_alpha%20gives%20ampicillin%20resistance>Checking if OmpA_omega_ΔA_alpha gives ampicillin resistance </a></li> |
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=10&arg0=19_August_2008&arg1=20_August_2008&arg2=21_August_2008&arg3=22_August_2008&arg4=25_August_2008&arg5=26_August_2008&arg6=27_August_2008&arg7=9_September_2008&arg8=10_September_2008&arg9=11_September_2008&name=Tests%20for%20ampicillin%20resistance%20given%20by%20protein%20added%20to%20medium">Tests for ampicillin resistance given by protein added to medium</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=10&arg0=19_August_2008&arg1=20_August_2008&arg2=21_August_2008&arg3=22_August_2008&arg4=25_August_2008&arg5=26_August_2008&arg6=27_August_2008&arg7=9_September_2008&arg8=10_September_2008&arg9=11_September_2008&name=Tests%20for%20ampicillin%20resistance%20given%20by%20protein%20added%20to%20medium">Tests for ampicillin resistance given by protein added to medium</a></li> | ||
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</ul> | </ul> | ||
</div> | </div> | ||
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| + | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=4_August_2008&arg1=5_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20expression>Checking OmpA_omega_ΔA_alpha expression</a></li> | ||
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</div> | </div> | ||
| - | <h3 | + | <h3 OnClick=switchMenu('bricks') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Preparation of BioBricks</h3> |
<div name="menu_item" id="bricks" style="display: none"> | <div name="menu_item" id="bricks" style="display: none"> | ||
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=17_September_2008&arg1=18_September_2008&name=Optimisation%20of%20primers%20for%20preparation%20of%20parts">Optimisation of primers for preparation of parts</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=17_September_2008&arg1=18_September_2008&name=Optimisation%20of%20primers%20for%20preparation%20of%20parts">Optimisation of primers for preparation of parts</a></li> | ||
| - | <li class="nb_toc1" | + | <li class="nb_toc1" OnClick=switchMenu('bricks-vect','submenu_item')>Preparation of vectors for BioBricks parts cloning</li> |
<div name="submenu_item" id="bricks-vect" style="display: none"> | <div name="submenu_item" id="bricks-vect" style="display: none"> | ||
<ul> | <ul> | ||
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</div> | </div> | ||
| - | <li class="nb_toc1" | + | <li class="nb_toc1" OnClick=switchMenu('bricks-coding','submenu_item')>Preparation of BioBricks parts (coding type)</li> |
<div name="submenu_item" id="bricks-coding" style="display: none"> | <div name="submenu_item" id="bricks-coding" style="display: none"> | ||
<ul> | <ul> | ||
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</div> | </div> | ||
| - | <li class="nb_toc1" | + | <li class="nb_toc1" OnClick=switchMenu('bricks-generator','submenu_item')>Preparation of BioBricks parts (coding type)</li> |
<div name="submenu_item" id="bricks-generator" style="display: none"> | <div name="submenu_item" id="bricks-generator" style="display: none"> | ||
<ul> | <ul> | ||
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| - | <h3 | + | <h3 OnClick=switchMenu('other') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Other activities</h3> |
<div name="menu_item" id="other" style="display: none"> | <div name="menu_item" id="other" style="display: none"> | ||
<ol> | <ol> | ||
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=8_September_2008&name=Our%20webpage%20has%20a%20completely%20new%20layout">Our webpage has a completely new layout</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=8_September_2008&name=Our%20webpage%20has%20a%20completely%20new%20layout">Our webpage has a completely new layout</a></li> | ||
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=11_September_2008&name=We%27re%20fighting%20hard%20to%20obtain%20funds%20for%20the%20visit%20on%20iGEM2008%20Jamboree%21">We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=11_September_2008&name=We%27re%20fighting%20hard%20to%20obtain%20funds%20for%20the%20visit%20on%20iGEM2008%20Jamboree%21">We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</a></li> | ||
| + | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=10_October_2008&name=Visit%20in%20US%20Embassy">Visit in US Embassy</a></li> | ||
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=23_October_2008&name=Sending%20our%20bricks%20to%20the%20Registry">Sending our bricks to the Registry</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=23_October_2008&name=Sending%20our%20bricks%20to%20the%20Registry">Sending our bricks to the Registry</a></li> | ||
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=24_October_2008&name=Computer%20meeting%20to%20finish%20our%20webpage">Computer meeting to finish our webpage</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=24_October_2008&name=Computer%20meeting%20to%20finish%20our%20webpage">Computer meeting to finish our webpage</a></li> | ||
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</ol> | </ol> | ||
</div> | </div> | ||
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| - | </td><td valign="top"> </html> | + | </td><td valign="top" style> </html> |
{| style="background:transparent;" | {| style="background:transparent;" | ||
|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=05 | title=Team:Warsaw/Calendar-Main}} | |{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=05 | title=Team:Warsaw/Calendar-Main}} | ||
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|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=09 | title=Team:Warsaw/Calendar-Main}} | |{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=09 | title=Team:Warsaw/Calendar-Main}} | ||
|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=10 | title=Team:Warsaw/Calendar-Main}} | |{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=10 | title=Team:Warsaw/Calendar-Main}} | ||
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|}<html> | |}<html> | ||
</td></tr></table> | </td></tr></table> | ||
| - | < | + | <hr width="100%"> |
| - | < | + | <h3 align="left" OnClick="switchMenu('protocols','menu_item')" onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Protocols</h3> |
| - | < | + | <div name="menu_item" id="protocols" style="display:none"> |
| - | < | + | |
| - | < | + | <ol> |
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o">Purification of His_Z_alpha and His_Z_omega</a></li> | ||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#A_a">Purification of His_A_alpha</a></li> | ||
| + | |||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Testing various hunter/prey combinations">Testing various hunter/prey combinations</a></li> | ||
| + | |||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Plasmid DNA isolation</a></li> | ||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">DNA isolation from agarose gel</a></li> | ||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">DNA purification after enzymatic reaction</a></li> | ||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#genomic_DNA_isolation">Genomic DNA isolation</a></li> | ||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">Preparation of chemocompetent bacteria</a></li> | ||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrocompetent">Preparation of electrocompetent bacteria</a></li> | ||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Chemotransformation</a></li> | ||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Electrotransformation</a></li> | ||
| + | |||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a></li> | ||
| + | |||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#blunting">Blunting</a></li> | ||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#concentrations">Standard concentrations of antibiotics and other supplements | ||
| + | </a></li> | ||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#rif">Rifampicin test | ||
| + | </a></li> | ||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">Removing 5' phosphate groups from DNA ends | ||
| + | </a></li> | ||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#taxi">TAXI protocol (Tet+Ap 30+X-Gal+IPTG) | ||
| + | </a></li> | ||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#bca">Protein concentration measurement (BCA method) | ||
| + | </a></li> | ||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">DNA digest | ||
| + | </a></li> | ||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR | ||
| + | </a></li> | ||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcl">Polymerase Chain Ligation (PCL) | ||
| + | </a></li> | ||
| + | |||
| + | </ol> | ||
| + | </div> | ||
| + | |||
| + | <h3 align="left"><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/primers">Primers</a></h3> | ||
| + | <h3 align="left"><a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors">Vectors</a></h3> | ||
| + | <h3 align="left" OnClick="switchMenu('strains','menu_item')" onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Strains</h3> | ||
| + | <div name="menu_item" id="strains" style="display:none;"> | ||
<h4><a name="rosetta">Rosetta(DE3)pLysS</a></h4> | <h4><a name="rosetta">Rosetta(DE3)pLysS</a></h4> | ||
| - | <p>F- ompT hsdSB(RB- mB-) gal dcm | + | <p>F- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR)</p> |
<h4><a name="top10">TOP10 (Invitrogen)</a></h4> | <h4><a name="top10">TOP10 (Invitrogen)</a></h4> | ||
| - | <p>F- mcrA | + | <p>F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- </p> |
| - | <h4><a name="mutD5">NR9458 ( | + | <h4><a name="mutD5">NR9458 (<a href="http://www.ibb.waw.pl/">Institute of Biochemistry and Biophysics</a> collection; in notebook called mutD5)</a></h4> |
| - | <p>ara thi | + | <p>ara thi Δ pro lac mutD5 zaf-13 ::Tn10</p> |
<h4><a name="gm">GM2163</a></h4> | <h4><a name="gm">GM2163</a></h4> | ||
<p>ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 | <p>ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 | ||
R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 | R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 | ||
hsdR2</p> | hsdR2</p> | ||
| - | + | </div> | |
| - | + | <h3 align="left" OnClick="switchMenu('javascript','menu_item')" onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">JavaScript tools developed to make work with iGEM notebook more comfortable</h3> | |
| + | <div name="menu_item" id="javascript" style="display: none"> | ||
| + | <ol> | ||
| + | <li><a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/NoAtribution">Author attribution analyzer</a></li> | ||
| + | <li><a href="https://2008.igem.org/Team:Warsaw/JSTest2">Inretactive Notebook topics list</a></li> | ||
| + | </ol> | ||
| + | </div> | ||
| + | </td> | ||
<td><img src="https://static.igem.org/mediawiki/2008/5/52/Spacer.gif" width="1" height="530" border="0" alt="" /></td> | <td><img src="https://static.igem.org/mediawiki/2008/5/52/Spacer.gif" width="1" height="530" border="0" alt="" /></td> | ||
</tr> | </tr> | ||
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name = typeof(name) != 'undefined' ? name : "menu_item"; | name = typeof(name) != 'undefined' ? name : "menu_item"; | ||
| - | + | //var m=document.getElementsByName(name); | |
| - | var m=document.getElementsByName(name); | + | var m=document.getElementsByTagName("div"); |
for (var i=0;i<m.length;i++) | for (var i=0;i<m.length;i++) | ||
{ | { | ||
| - | + | //alert(m[i].getAttribute("name")); | |
| - | if(m[i].style.display!="none") if (m[i].id!=obj) m[i].style.display="none"; | + | if (m[i].getAttribute("name")==name) if(m[i].style.display!="none") if (m[i].id!=obj) m[i].style.display="none"; |
} | } | ||
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var el = document.getElementById(obj); | var el = document.getElementById(obj); | ||
if ( el.style.display != "none" ) { | if ( el.style.display != "none" ) { | ||
| - | el.style.display = "none"; | + | //el.style.display = "none"; |
| + | if (document.getElementById) { // DOM3 = IE5, NS6 | ||
| + | document.getElementById(obj).style.display = 'none'; | ||
} | } | ||
else { | else { | ||
| - | + | if (document.layers) { // Netscape 4 | |
| + | document.obj.display = 'none'; | ||
| + | } | ||
| + | else { // IE 4 | ||
| + | document.all.obj.style.display = 'none'; | ||
| + | } | ||
} | } | ||
| + | } | ||
| + | else { | ||
| + | //el.style.display = "block"; | ||
| + | if (document.getElementById) { // DOM3 = IE5, NS6 | ||
| + | document.getElementById(obj).style.display = 'block'; | ||
| + | } | ||
| + | else { | ||
| + | if (document.layers) { // Netscape 4 | ||
| + | document.obj.display = 'block'; | ||
| + | } | ||
| + | else { // IE 4 | ||
| + | document.all.obj.style.display = 'block'; | ||
| + | } | ||
| + | } | ||
| + | } | ||
| + | |||
} | } | ||
| + | |||
| + | qs=parent.document.URL; | ||
| + | if (qs.indexOf('#')>0) document.getElementById("strains").style.display = 'block'; | ||
//--> | //--> | ||
</script> | </script> | ||
</html> | </html> | ||
Latest revision as of 06:51, 30 October 2008
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ProtocolsPrimersVectorsStrainsJavaScript tools developed to make work with iGEM notebook more comfortable |
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