Wiki/Team:Warsaw/igem notebook.htm

From 2008.igem.org

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<table class="month"><tr>
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<td align=center width=70%><h1><p style="font-size: 16px; text-align: center; text-decoration: bold;">Table of contents</p></h1></td><td>&nbsp;</td></tr>
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<td align=center width=70%><h1><p style="font-size: 16px; text-align: center; text-decoration: bold;">Table of contents </p></h1></td><td>&nbsp;</td></tr>
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<h3><a onClick=switchMenu('one-armed-vect')> Preparation of one-armed bandit vectors</a></h3>
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<h3 onClick=switchMenu('one-armed-vect') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';"> Preparation of one-armed bandit vectors</h3>
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<div name="menu_item" style="display: none" id="one-armed-vect" >
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<div name="menu_item" style="display: none" id="one-armed-vect">
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<h3><a OnClick=switchMenu('one-armed-tests')> One-armed bandit tests</a></h3>
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<h3 OnClick=switchMenu('one-armed-tests') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';"> One-armed bandit tests</h3>
<div name="menu_item" style="display: none" id="one-armed-tests">
<div name="menu_item" style="display: none" id="one-armed-tests">
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<h3><a OnClick=switchMenu('hunter-vect')>Preparation of vectors for 'hunter' part of 'hunter and prey'</a></h3>
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<h3 OnClick=switchMenu('hunter-vect') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Preparation of vectors for 'hunter' part of 'hunter and prey'</h3>
<div name="menu_item" id="hunter-vect" style="display: none">
<div name="menu_item" id="hunter-vect" style="display: none">
<ol>
<ol>
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<li class="nb_toc1"><a OnClick=switchMenu('prot_A','submenu_item')>Preparation of vectors with protein A derivatives fusions</a></li>
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<li class="nb_toc1" OnClick=switchMenu('prot_A','submenu_item')>Preparation of vectors with protein A derivatives fusions</li>
<div name="submenu_item" id="prot_A" style="display: none">
<div name="submenu_item" id="prot_A" style="display: none">
<ul>
<ul>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=7_July_2008&arg1=8_July_2008&arg2=14_July_2008&arg3=15_July_2008&arg4=16_July_2008&arg5=21_July_2008&arg6=22_July_2008&arg7=23_July_2008&arg8=28_July_2008&name=Cloning%20omega-A%20fusion%20on%20pKS%20%28second%20attempt%29>Cloning omega-A fusion on pKS (second attempt)</a></li>
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=7_July_2008&arg1=8_July_2008&arg2=14_July_2008&arg3=15_July_2008&arg4=16_July_2008&arg5=21_July_2008&arg6=22_July_2008&arg7=23_July_2008&arg8=28_July_2008&name=Cloning%20omega-A%20fusion%20on%20pKS%20%28second%20attempt%29>Cloning omega-A fusion on pKS (second attempt)</a></li>
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Preparation%20of%20construct%20pKS%20with%20A%20protein>Preparation of construct pKS with A protein </a></li>
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Preparation%20of%20construct%20pKS%20with%20A%20protein>Preparation of construct pKS with A protein </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=14_July_2008&arg1=15_July_2008&name=Preparation%20of%20alpha%20A%20conctruct>Preparation of alpha A conctruct </a></li>
 
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=21_July_2008&arg1=22_July_2008&arg2=23_July_2008&arg3=24_July_2008&arg4=25_July_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20OmpA%20constructs>Cloning of protein A DNA to OmpA constructs </a></li>
 
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=18_August_2008&arg1=19_August_2008&arg2=20_August_2008&arg3=21_August_2008&arg4=22_August_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20GeneArt%20plasmid>Cloning of protein A DNA to GeneArt plasmid </a></li>
 
 +
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=21_July_2008&arg1=22_July_2008&arg2=23_July_2008&arg3=24_July_2008&arg4=25_July_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20OmpA%20constructs>Cloning of protein A DNA to OmpA constructs </a></li>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=7&amp;arg0=22_September_2008&amp;arg1=23_September_2008&amp;arg2=24_September_2008&amp;arg3=25_September_2008&amp;arg4=26_September_2008&amp;arg5=27_September_2008&amp;arg6=30_September_2008&amp;name=Mutagenesis%20of%20protein%20A">Mutagenesis of protein A</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=7&amp;arg0=22_September_2008&amp;arg1=23_September_2008&amp;arg2=24_September_2008&amp;arg3=25_September_2008&amp;arg4=26_September_2008&amp;arg5=27_September_2008&amp;arg6=30_September_2008&amp;name=Mutagenesis%20of%20protein%20A">Mutagenesis of protein A</a></li>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=2&amp;arg0=24_September_2008&amp;arg1=25_September_2008&amp;name=Preparation%20of%20alpha_A%20construct">Preparation of alpha_A construct</a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=14_July_2008&arg1=15_July_2008&name=Preparation%20of%20alpha%20A%20conctruct>Preparation of alpha A construct #1</a></li>
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 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=2&amp;arg0=24_September_2008&amp;arg1=25_September_2008&amp;name=Preparation%20of%20alpha_A%20construct">Preparation of alpha_A construct #2</a></li>
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</ul>
</ul>
</div>
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<li class="nb_toc1"><a OnClick=switchMenu('delta_A','submenu_item')>Preparation of vectors with B-domain of protein A derivatives fusions</a></li>
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<li class="nb_toc1" OnClick=switchMenu('delta_A','submenu_item')>Preparation of vectors with B-domain of protein A derivatives fusions</li>
<div name="submenu_item" id="delta_A" style="display: none">
<div name="submenu_item" id="delta_A" style="display: none">
<ul>
<ul>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=29_July_2008&arg1=30_July_2008&arg2=31_July_2008&name=Cloning%20of%20omega_%26Delta%3BA%20DNA%20fragment%20to%20pACYC177%20OmpA_alpha>Cloning of omega_&Delta;A DNA fragment to pACYC177 OmpA_alpha </a></li>
 
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=30_July_2008&arg1=31_July_2008&arg2=1_August_2008&arg3=4_August_2008&arg4=5_August_2008&name=Cloning%20of%20truncated%20fragment%20of%20protein%20A%20%28%26Delta%3BA%29>Cloning of truncated fragment of protein A (&Delta;A) </a></li>
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=30_July_2008&arg1=31_July_2008&arg2=1_August_2008&arg3=4_August_2008&arg4=5_August_2008&name=Cloning%20of%20truncated%20fragment%20of%20protein%20A%20%28%26Delta%3BA%29>Cloning of truncated fragment of protein A (&Delta;A) </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=29_July_2008&arg1=30_July_2008&arg2=31_July_2008&name=Cloning%20of%20omega_%26Delta%3BA%20DNA%20fragment%20to%20pACYC177%20OmpA_alpha>Cloning of omega_&Delta;A DNA fragment to pACYC177 OmpA_alpha </a></li>
 +
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&arg3=7_August_2008&name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct>Preparing pACYC177 OmpA_omega_&Delta;A construct </a></li>
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&arg3=7_August_2008&name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct>Preparing pACYC177 OmpA_omega_&Delta;A construct </a></li>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=4&amp;arg0=4_August_2008&amp;arg1=5_August_2008&amp;arg2=6_August_2008&amp;arg3=7_August_2008&amp;name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct">Preparing pACYC177 OmpA_omega_&Delta;A construct</a>
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</li>
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</ul>
</ul>
</div>
</div>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=14_July_2008&arg1=15_July_2008&arg2=16_July_2008&arg3=17_July_2008&arg4=18_July_2008&arg5=19_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20OmpA%20constructs>Cloning of protein Z DNA to OmpA constructs </a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=9&amp;arg0=13_August_2008&amp;arg1=14_August_2008&amp;arg2=18_August_2008&amp;arg3=19_August_2008&amp;arg4=20_August_2008&amp;arg5=21_August_2008&amp;arg6=22_August_2008&amp;arg7=25_August_2008&amp;arg8=26_August_2008&amp;name=Cloning%20of%20alpha%20to%20pACYC177%20OmpA_A_omega%20and%20pACYC177%20OmpA_Z_omega">Cloning of alpha to pACYC177 OmpA_A_omega and pACYC177 OmpA_Z_omega</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=9&amp;arg0=13_August_2008&amp;arg1=14_August_2008&amp;arg2=18_August_2008&amp;arg3=19_August_2008&amp;arg4=20_August_2008&amp;arg5=21_August_2008&amp;arg6=22_August_2008&amp;arg7=25_August_2008&amp;arg8=26_August_2008&amp;name=Cloning%20of%20alpha%20to%20pACYC177%20OmpA_A_omega%20and%20pACYC177%20OmpA_Z_omega">Cloning of alpha to pACYC177 OmpA_A_omega and pACYC177 OmpA_Z_omega</a></li>
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<h3><a OnClick=switchMenu('prey-vect')>Preparation of vectors for 'prey' part of 'hunter and prey'</a></h3>
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<h3 OnClick=switchMenu('prey-vect') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Preparation of vectors for 'prey' part of 'hunter and prey'</h3>
<div name="menu_item" id="prey-vect" style="display: none">
<div name="menu_item" id="prey-vect" style="display: none">
<ol>
<ol>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=17_July_2008&arg1=18_July_2008&arg2=21_July_2008&arg3=22_July_2008&arg4=23_July_2008&arg5=24_July_2008&arg6=25_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-omega%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA </a></li>
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=17_July_2008&arg1=18_July_2008&arg2=21_July_2008&arg3=22_July_2008&arg4=23_July_2008&arg5=24_July_2008&arg6=25_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-omega%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=19_August_2008&arg1=22_August_2008&arg2=25_August_2008&arg3=26_August_2008&arg4=27_August_2008&arg5=28_August_2008&arg6=29_August_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20pET15b%20OmpA-alpha%20plasmid>Cloning of protein A DNA to pET15b OmpA-alpha plasmid </a></li>
 
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=14_July_2008&arg1=15_July_2008&arg2=16_July_2008&arg3=17_July_2008&arg4=18_July_2008&arg5=19_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20OmpA%20constructs>Cloning of protein Z DNA to OmpA constructs </a></li>
 
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-alpha%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpA </a></li>
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-alpha%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpA </a></li>
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<h3><a OnClick=switchMenu('prey-purif')>Purification of prey proteins</a></h3>
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<h3 OnClick=switchMenu('prey-purif') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Purification of prey proteins</h3>
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<div name="menu_item" id="prey-purif" style="display: none">
<ol>
<ol>
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<h3><a OnClick="switchMenu('hunter-tests')">'Hunter and prey' tests</h3>
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<h3 OnClick="switchMenu('hunter-tests')" onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">'Hunter and prey' tests</h3>
<div name="menu_item" id="hunter-tests" style="display: none">
<div name="menu_item" id="hunter-tests" style="display: none">
<ol>
<ol>
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<li class="nb_toc1"><a OnClick="switchMenu('expr-tests','submenu_item')">Protein expression tests</li>
 
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<div name="submenu_item" id="expr-tests" style="display: none">
 
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<ul>
 
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=4_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20and%20OmpA_A_alpha%20expression>Checking OmpA_omega_&Delta;A_alpha and OmpA_A_alpha expression </a></li>
 
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=5_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20expression>Checking OmpA_omega_&Delta;A_alpha expression </a></li>
 
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</ul>
 
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</div>
 
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<li class="nb_toc1"><a OnClick="switchMenu('resist-tests','submenu_item')">Ampicillin-resistance tests</li>
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<li class="nb_toc1" OnClick="switchMenu('resist-tests','submenu_item')">Ampicillin-resistance tests</li>
<div name="submenu_item" id="resist-tests" style="display: none">
<div name="submenu_item" id="resist-tests" style="display: none">
<ul>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=4_August_2008&arg1=5_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20expression>Checking OmpA_omega_ΔA_alpha expression</a></li>
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<h3><a OnClick=switchMenu('bricks')>Preparation of BioBricks</a></h3>
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<h3 OnClick=switchMenu('bricks') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Preparation of BioBricks</h3>
<div name="menu_item" id="bricks" style="display: none">
<div name="menu_item" id="bricks" style="display: none">
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=2&amp;arg0=17_September_2008&amp;arg1=18_September_2008&amp;name=Optimisation%20of%20primers%20for%20preparation%20of%20parts">Optimisation of primers for preparation of parts</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=2&amp;arg0=17_September_2008&amp;arg1=18_September_2008&amp;name=Optimisation%20of%20primers%20for%20preparation%20of%20parts">Optimisation of primers for preparation of parts</a></li>
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<li class="nb_toc1"><a OnClick=switchMenu('bricks-vect','submenu_item')>Preparation of vectors for BioBricks parts cloning</a></li>
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<li class="nb_toc1" OnClick=switchMenu('bricks-vect','submenu_item')>Preparation of vectors for BioBricks parts cloning</li>
<div name="submenu_item" id="bricks-vect" style="display: none">
<div name="submenu_item" id="bricks-vect" style="display: none">
<ul>
<ul>
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</div>
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<li class="nb_toc1"><a OnClick=switchMenu('bricks-coding','submenu_item')>Preparation of BioBricks parts (coding type)</a></li>
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<li class="nb_toc1" OnClick=switchMenu('bricks-coding','submenu_item')>Preparation of BioBricks parts (coding type)</li>
<div name="submenu_item" id="bricks-coding" style="display: none">
<div name="submenu_item" id="bricks-coding" style="display: none">
<ul>
<ul>
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<li class="nb_toc1"><a OnClick=switchMenu('bricks-generator','submenu_item')>Preparation of BioBricks parts (coding type)</a></li>
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<li class="nb_toc1" OnClick=switchMenu('bricks-generator','submenu_item')>Preparation of BioBricks parts (coding type)</li>
<div name="submenu_item" id="bricks-generator" style="display: none">
<div name="submenu_item" id="bricks-generator" style="display: none">
<ul>
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<h3><a OnClick=switchMenu('other')>Other activities</a></h3>
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<h3 OnClick=switchMenu('other') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Other activities</h3>
<div name="menu_item" id="other" style="display: none">
<div name="menu_item" id="other" style="display: none">
<ol>
<ol>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=8_September_2008&amp;name=Our%20webpage%20has%20a%20completely%20new%20layout">Our webpage has a completely new layout</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=8_September_2008&amp;name=Our%20webpage%20has%20a%20completely%20new%20layout">Our webpage has a completely new layout</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=11_September_2008&amp;name=We%27re%20fighting%20hard%20to%20obtain%20funds%20for%20the%20visit%20on%20iGEM2008%20Jamboree%21">We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=11_September_2008&amp;name=We%27re%20fighting%20hard%20to%20obtain%20funds%20for%20the%20visit%20on%20iGEM2008%20Jamboree%21">We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</a></li>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=10_October_2008&amp;name=Visit%20in%20US%20Embassy">Visit in US Embassy</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=23_October_2008&amp;name=Sending%20our%20bricks%20to%20the%20Registry">Sending our bricks to the Registry</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=23_October_2008&amp;name=Sending%20our%20bricks%20to%20the%20Registry">Sending our bricks to the Registry</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=24_October_2008&amp;name=Computer%20meeting%20to%20finish%20our%20webpage">Computer meeting to finish our webpage</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=24_October_2008&amp;name=Computer%20meeting%20to%20finish%20our%20webpage">Computer meeting to finish our webpage</a></li>
-
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=10_October_2008&amp;name=Visit%20in%20US%20Embassy">Visit in US Embassy</a></li>
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<hr width="100%">
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<h2 align="left"><a href=https://2008.igem.org/Wiki/Team:Warsaw/protocols_list>Protocols</a></h2>
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<h3 align="left" OnClick="switchMenu('protocols','menu_item')" onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Protocols</h3>
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<div name="menu_item" id="protocols" style="display:none">
 +
 
 +
<ol>
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o">Purification of His_Z_alpha and His_Z_omega</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#A_a">Purification of His_A_alpha</a></li>
 +
 
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Testing various hunter/prey combinations">Testing various hunter/prey combinations</a></li>
 +
 
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Plasmid DNA isolation</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">DNA isolation from agarose gel</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">DNA purification after enzymatic reaction</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#genomic_DNA_isolation">Genomic DNA isolation</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">Preparation of chemocompetent bacteria</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrocompetent">Preparation of electrocompetent bacteria</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Chemotransformation</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Electrotransformation</a></li>
 +
 
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a></li>
 +
 
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#blunting">Blunting</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#concentrations">Standard concentrations of antibiotics and other supplements
 +
</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#rif">Rifampicin test
 +
</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">Removing 5' phosphate groups from DNA ends
 +
</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#taxi">TAXI protocol (Tet+Ap 30+X-Gal+IPTG)
 +
</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#bca">Protein concentration measurement (BCA method)
 +
</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">DNA digest
 +
</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR
 +
</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcl">Polymerase Chain Ligation (PCL)
 +
</a></li>
 +
 
 +
</ol>
 +
</div>
-
<h2 align="left"><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/primers">Primers</a></h2>
+
<h3 align="left"><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/primers">Primers</a></h3>
-
<h2 align="left"><a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors">Vectors</a></h2>
+
<h3 align="left"><a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors">Vectors</a></h3>
-
<h2 align="left"><a href="https://2008.igem.org/Wiki/Team:Warsaw/scripts">JavaScript tools developed to make work with iGEM notebook more comfortable</a></h2>
+
<h3 align="left" OnClick="switchMenu('strains','menu_item')" onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Strains</h3>
-
<h2 align="left">Strains</h2>
+
<div name="menu_item" id="strains" style="display:none;">
<h4><a name="rosetta">Rosetta(DE3)pLysS</a></h4>
<h4><a name="rosetta">Rosetta(DE3)pLysS</a></h4>
<p>F- ompT hsdSB(RB- mB-) gal dcm &lambda;(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR)</p>
<p>F- ompT hsdSB(RB- mB-) gal dcm &lambda;(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR)</p>
<h4><a name="top10">TOP10 (Invitrogen)</a></h4>
<h4><a name="top10">TOP10 (Invitrogen)</a></h4>
<p>F- mcrA &Delta;(mrr-hsdRMS-mcrBC) &phi;80lacZ&Delta;M15 &Delta;lacX74 nupG recA1 araD139 &Delta;(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 &lambda;- </p>
<p>F- mcrA &Delta;(mrr-hsdRMS-mcrBC) &phi;80lacZ&Delta;M15 &Delta;lacX74 nupG recA1 araD139 &Delta;(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 &lambda;- </p>
-
<h4><a name="mutD5">NR9458 (IBB collection; in notebook called mutD5)</a></h4>
+
<h4><a name="mutD5">NR9458 (<a href="http://www.ibb.waw.pl/">Institute of Biochemistry and Biophysics</a> collection; in notebook called mutD5)</a></h4>
<p>ara thi &Delta; pro lac mutD5 zaf-13 ::Tn10</p>
<p>ara thi &Delta; pro lac mutD5 zaf-13 ::Tn10</p>
<h4><a name="gm">GM2163</a></h4>
<h4><a name="gm">GM2163</a></h4>
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R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1
R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1
hsdR2</p>
hsdR2</p>
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</div>
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</div></td>
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<h3 align="left" OnClick="switchMenu('javascript','menu_item')" onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">JavaScript tools developed to make work with iGEM notebook more comfortable</h3>
 +
<div name="menu_item" id="javascript" style="display: none">
 +
<ol>
 +
<li><a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/NoAtribution">Author attribution analyzer</a></li>
 +
<li><a href="https://2008.igem.org/Team:Warsaw/JSTest2">Inretactive Notebook topics list</a></li>
 +
</ol>
 +
</div>
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</td>
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Latest revision as of 06:51, 30 October 2008

Gallery Bricks Notebook Team Project Home

Table of contents

 

Preparation of one-armed bandit vectors

One-armed bandit tests

Preparation of vectors for 'hunter' part of 'hunter and prey'

Preparation of vectors for 'prey' part of 'hunter and prey'

Purification of prey proteins

'Hunter and prey' tests

Preparation of BioBricks

Other activities

May
MTWTFSS
      [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_May_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_May_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_May_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_May_2008 4]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/5_May_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_May_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_May_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_May_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_May_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_May_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_May_2008 11]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/12_May_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_May_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_May_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_May_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_May_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_May_2008 18]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/19_May_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_May_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_May_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_May_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_May_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_May_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_May_2008 25]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/26_May_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_May_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_May_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_May_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_May_2008 30] [http://2008.igem.org/Team:Warsaw/Calendar-Main/31_May_2008 31]
June
MTWTFSS
            [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_June_2008 1]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/2_June_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_June_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_June_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_June_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_June_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_June_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_June_2008 8]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/9_June_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_June_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_June_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_June_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_June_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_June_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_June_2008 15]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/16_June_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_June_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_June_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_June_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_June_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_June_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_June_2008 22]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/23_June_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_June_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_June_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_June_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_June_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_June_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_June_2008 29]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/30_June_2008 30]
July
MTWTFSS
  [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_July_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_July_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_July_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_July_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_July_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_July_2008 6]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/7_July_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_July_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_July_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_July_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_July_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_July_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_July_2008 13]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/14_July_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_July_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_July_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_July_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_July_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_July_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_July_2008 20]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/21_July_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_July_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_July_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_July_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_July_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_July_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_July_2008 27]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/28_July_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_July_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_July_2008 30] [http://2008.igem.org/Team:Warsaw/Calendar-Main/31_July_2008 31]
August
MTWTFSS
        [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_August_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_August_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_August_2008 3]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/4_August_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_August_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_August_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_August_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_August_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_August_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_August_2008 10]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/11_August_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_August_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_August_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_August_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_August_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_August_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_August_2008 17]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/18_August_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_August_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_August_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_August_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_August_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_August_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_August_2008 24]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/25_August_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_August_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_August_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_August_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_August_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_August_2008 30] [http://2008.igem.org/Team:Warsaw/Calendar-Main/31_August_2008 31]
September
MTWTFSS
[http://2008.igem.org/Team:Warsaw/Calendar-Main/1_September_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_September_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_September_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_September_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_September_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_September_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_September_2008 7]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/8_September_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_September_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_September_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_September_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_September_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_September_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_September_2008 14]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/15_September_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_September_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_September_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_September_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_September_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_September_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_September_2008 21]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/22_September_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_September_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_September_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_September_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_September_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_September_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_September_2008 28]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/29_September_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_September_2008 30]
October
MTWTFSS
    [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_October_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_October_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_October_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_October_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_October_2008 5]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/6_October_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_October_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_October_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_October_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_October_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_October_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_October_2008 12]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/13_October_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_October_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_October_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_October_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_October_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_October_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_October_2008 19]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/20_October_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_October_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_October_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_October_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_October_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_October_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_October_2008 26]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/27_October_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_October_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_October_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_October_2008 30] [http://2008.igem.org/Team:Warsaw/Calendar-Main/31_October_2008 31]

Protocols

Primers

Vectors

Strains

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