Team:University of Ottawa/24 June 2008

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(New page: __TOC__ ==Today in the Lab== '''Matt''' :'''Genomic DNA extraction''' ::<li> Mila showed me how to do a genomic DNA extraction of the four YPH500 colonies. ::<li> Absorbance of colony A wh...)
 
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__TOC__
__TOC__
==Today in the Lab==
==Today in the Lab==
Line 4: Line 72:
:'''Genomic DNA extraction'''
:'''Genomic DNA extraction'''
::<li> Mila showed me how to do a genomic DNA extraction of the four YPH500 colonies.
::<li> Mila showed me how to do a genomic DNA extraction of the four YPH500 colonies.
-
::<li> Absorbance of colony A when measuring DNA concentration was found to be 0.6641 - that colony was then chosen for PCR amplification of the PTP2 cassette.
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::<li> Genomic DNA concentration of colony A was found to be 830.125 ng/ul  - that colony was then chosen for PCR amplification of the PTP2 cassette.
::<li> The PCR amplification will be performed tomorrow morning.
::<li> The PCR amplification will be performed tomorrow morning.
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'''Dan'''
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:'''Miniprep
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::<li> Performed a miniprep to obtain  pSSA42, S1, D12, and T123 plasmids.
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::<li> Taking the absorbances of the DNA indicated good concentrations.
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::<li> Digested the S1, D12 and T123 plasmids with NcoI and EcoRI
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::<li> Digested pSSA42 with HindIII
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::<li> Ran both plasmids on a gel and obtained expected results but forgot to add marker :(
 +
::<li> Redigested plasmids overnight, will run gel again tomorrow

Latest revision as of 22:56, 28 October 2008

Untitled Document

 

 

Contents

Today in the Lab

Matt

Genomic DNA extraction
  • Mila showed me how to do a genomic DNA extraction of the four YPH500 colonies.
  • Genomic DNA concentration of colony A was found to be 830.125 ng/ul - that colony was then chosen for PCR amplification of the PTP2 cassette.
  • The PCR amplification will be performed tomorrow morning.

    • Dan

    Miniprep
  • Performed a miniprep to obtain pSSA42, S1, D12, and T123 plasmids.
  • Taking the absorbances of the DNA indicated good concentrations.
  • Digested the S1, D12 and T123 plasmids with NcoI and EcoRI
  • Digested pSSA42 with HindIII
  • Ran both plasmids on a gel and obtained expected results but forgot to add marker :(
  • Redigested plasmids overnight, will run gel again tomorrow
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/24_June_2008"