Team:Waterloo/Notebook/Oligos

From 2008.igem.org

(Difference between revisions)
 
(6 intermediate revisions not shown)
Line 9: Line 9:
|}
|}
-
{| border="1" cellpadding="2"
+
{| width="100%" border="1" cellpadding="2"
-
!width="500"|Description
+
! Description !! Oligo Name !! Sequence !! Length
-
!width="500"|Oligo Name
+
-
!width="500"|Sequence
+
-
!width="500"|Length
+
|-
|-
|BioBrick sequencing/colPCR primer (fwd)  || iGEMvf2 || TGCCACCTGACGTCTAAGAA || 20
|BioBrick sequencing/colPCR primer (fwd)  || iGEMvf2 || TGCCACCTGACGTCTAAGAA || 20
Line 45: Line 42:
|T7gene6 ORF (rev)  || T7gs-R || CTGCAGCGGCCGCTACTAGTATTATTACGGTCTCCACAGGTAAATCTC || 48
|T7gene6 ORF (rev)  || T7gs-R || CTGCAGCGGCCGCTACTAGTATTATTACGGTCTCCACAGGTAAATCTC || 48
|-
|-
-
|  || T7g6-RXO-RBS || CTAGTATTTCTCCTCTTTCTCTAGTATTATTACGGTCTCCACAGGTAAATCTC || 53
+
|T7gene6 ORF rev; for joining to PmeI with crossover PCR || T7g6-RXO-RBS || CTAGTATTTCTCCTCTTTCTCTAGTATTATTACGGTCTCCACAGGTAAATCTC || 53
|-
|-
-
|  || RBSpmeI-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG || 61
+
|PmeI ORF (fwd; for adding prefix-RBS) || RBSpmeI-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG || 61
|-
|-
-
| || RBSpmeI-FXO || TACTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG || 48
+
|PmeI ORF (fwd; for adding prefix-RBS and joining to T7gene6 by crossover PCR) || RBSpmeI-FXO || TACTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG || 48
|-
|-
-
|  || pmeI-R || CTGCAGCGGCCGCTACTAGTATTATTAAACCGTACTTCGCCCCCG || 45
+
|PmeI ORF (rev; TAATAA stop codon-suffix) || pmeI-R || CTGCAGCGGCCGCTACTAGTATTATTAAACCGTACTTCGCCCCCG || 45
|-
|-
-
| || R0011-F || GAATTCCGGCCGCTTCTAGAGAATTGTGAGCGGATAAC || 38
+
|Primer for part R0011 (Plac-PL hybrid promoter; fwd) || R0011-F || GAATTCCGGCCGCTTCTAGAGAATTGTGAGCGGATAAC || 38
|-
|-
-
| || R0011RBS-RXO || CTAGTATTTCTCCTCTTTCTCTAGTATGTGCTCAGTATCTTGTTATC || 47
+
|Primer for part R0011 (Plac-PL hybrid promoter; rev for joining to ORFs with RBS by crossover PCR)|| R0011RBS-RXO || CTAGTATTTCTCCTCTTTCTCTAGTATGTGCTCAGTATCTTGTTATC || 47
|-
|-
-
| || RBSC0051-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC || 59
+
|CI ORF (fwd; prefix-RBS) || RBSC0051-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC || 59
|-
|-
-
| || RBSC0051-FXO || TACTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC || 46
+
|CI ORF (fwd; prefix-RBS for joining to R0011 promoter by crossover PCR) || RBSC0051-FXO || TACTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC || 46
|-
|-
-
|  || C0051-R || CTGCAGCGGCCGCTACTAGTATTATTAAGCTACTAAAGCG || 40
+
|CI ORF (rev; suffix adapter) || C0051-R || CTGCAGCGGCCGCTACTAGTATTATTAAGCTACTAAAGCG || 40
|-
|-
-
|  || C0051-RXO || GATGCCTGGCTCTAGTATTATTAAGCTACTAAAGCG || 36
+
|CI ORF (rev; for joining to transcriptional terminator (b0015) by crossover PCR) || C0051-RXO || GATGCCTGGCTCTAGTATTATTAAGCTACTAAAGCG || 36
|-
|-
-
|  || B0015-FXO || TAATAATACTAGAGCCAGGCATCAAATAAAACG || 33
+
|Transcriptional terminator (B0015; fwd; for joining to ORFs by crossover PCR) || B0015-FXO || TAATAATACTAGAGCCAGGCATCAAATAAAACG || 33
|-
|-
-
|  || B0015-R || CTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCC || 39
+
|Transcriptional terminator (B0015; rev) || B0015-R || CTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCC || 39
|-
|-
-
|  || RFP-RBS-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACG || 58
+
|RFP ORF (fwd; prefix-RBS) || RFP-RBS-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACG || 58
|-
|-
-
| || RFP-RXO || GATGCCTGGCTCTAGTATTATTAAGCACCGGTGG || 34
+
|RFP ORF (fwd; prefix-RBS for joining to transcriptional terminator by crossover PCR ) || RFP-RXO || GATGCCTGGCTCTAGTATTATTAAGCACCGGTGG || 34
|-
|-
-
| || Plac-RBS-F || GAATTCGCGGCCGCTTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAACAAGATACTGAGCACATACTAGAGAAAGAGGAGAAATACTAG || 103
+
|Plac (r0011; fwd; for adding lac promoter and RBS to ORFs by PCR)  || Plac-RBS-F || GAATTCGCGGCCGCTTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAACAAGATACTGAGCAC ATACTAGAGAAAGAGGAGAAATACTAG || 103
|}
|}
 +
<br>

Latest revision as of 03:57, 30 October 2008

Home The Team The Project Parts Submitted to the Registry Modeling Notebook Sponsors
Description Oligo Name Sequence Length
BioBrick sequencing/colPCR primer (fwd) iGEMvf2 TGCCACCTGACGTCTAAGAA 20
BioBrick sequencing/colPCR primer (rev) iGEMvr ATTACCGCCTTTGAGTGAGC 20
Primer for Lac promoter (fwd) PLacF TTATGCTTCCGGCTCGTATG 20
Primer for {BioBrick Prefix}-{B0034} (rev; for use with iGEMvf2) iGEMpre+rbs TTTCTCCTCTTTCTCTAGATGCGG 24
Primer for {BioBrick Prefix}-{B0034} (fwd; for use with iGEMvr) PrefixRBS-F GGCCGCTTCTAGAGAAAGAGG 21
Gene 5 ORF from T7 phage (fwd; Prefix adapter) T7gene5-F CAATGGAATTCGCGGCCGCTTCTAGATGATCGTTTCTGACATCGAA 46
Gene 5 ORF from T7 phage (rev; Suffix adapter) T7gene5-R CAATTTCTGCAGCGGCCGCTACTAGTATCAGTGGCAAATCGCCCAATTAGG 51
Gene 6 ORF from T7 phage (fwd; Prefix adapter) T7gene6-F CAATGGAATTCGCGGCCGCTTCTAGATGGCACTTCTTGACCTTAAA 46
Gene 6 ORF from T7 phage (rev; Suffix adapter) T7gene6-R CAATTTCTGCAGCGGCCGCTACTAGTACTACGGTCTCCACAGG 43
PmeIR ORF (fwd) PmeIR-F ATCCGGAATTCGCGGCGCTTTCTAGATGACAACAAACTCCCCCTCAGACG 50
PmeIR ORF (rev) PmelR-R CGCAAAACTGCAGCGGCCGCTACTAGTATCAAACCGTACTTCGCCCCCGTTG 52
pRecA oligo (sense strand; produces BioBrick-compatible EcoRI/SpeI overhangs when annealed to recAR) recAF AATTCGCGGCCGCTTCTAGAGTACAAAACACTTGATACTGTATATATATACAGTATAATTGCTTCAACATA 71
pRecA oligo (antisense strand; produces BioBrick-compatible EcoRI/SpeI overhangs when annealed to recAR) recAR CTAGTATGTTGAAGCAATTATACTGTATATATATACAGTATCAAGTGTTTTGTACTCTAGAAGCGGCCGCG 71
{B0034}-{T7gene6 ORF} (fwd; for adding {Prefix-RBS} onto the gene 6 ORF by PCR) RBST7g6-F GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCACTTCTTGACCTTAAAC 61
T7gene6 ORF (rev) T7gs-R CTGCAGCGGCCGCTACTAGTATTATTACGGTCTCCACAGGTAAATCTC 48
T7gene6 ORF rev; for joining to PmeI with crossover PCR T7g6-RXO-RBS CTAGTATTTCTCCTCTTTCTCTAGTATTATTACGGTCTCCACAGGTAAATCTC 53
PmeI ORF (fwd; for adding prefix-RBS) RBSpmeI-F GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG 61
PmeI ORF (fwd; for adding prefix-RBS and joining to T7gene6 by crossover PCR) RBSpmeI-FXO TACTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG 48
PmeI ORF (rev; TAATAA stop codon-suffix) pmeI-R CTGCAGCGGCCGCTACTAGTATTATTAAACCGTACTTCGCCCCCG 45
Primer for part R0011 (Plac-PL hybrid promoter; fwd) R0011-F GAATTCCGGCCGCTTCTAGAGAATTGTGAGCGGATAAC 38
Primer for part R0011 (Plac-PL hybrid promoter; rev for joining to ORFs with RBS by crossover PCR) R0011RBS-RXO CTAGTATTTCTCCTCTTTCTCTAGTATGTGCTCAGTATCTTGTTATC 47
CI ORF (fwd; prefix-RBS) RBSC0051-F GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC 59
CI ORF (fwd; prefix-RBS for joining to R0011 promoter by crossover PCR) RBSC0051-FXO TACTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC 46
CI ORF (rev; suffix adapter) C0051-R CTGCAGCGGCCGCTACTAGTATTATTAAGCTACTAAAGCG 40
CI ORF (rev; for joining to transcriptional terminator (b0015) by crossover PCR) C0051-RXO GATGCCTGGCTCTAGTATTATTAAGCTACTAAAGCG 36
Transcriptional terminator (B0015; fwd; for joining to ORFs by crossover PCR) B0015-FXO TAATAATACTAGAGCCAGGCATCAAATAAAACG 33
Transcriptional terminator (B0015; rev) B0015-R CTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCC 39
RFP ORF (fwd; prefix-RBS) RFP-RBS-F GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACG 58
RFP ORF (fwd; prefix-RBS for joining to transcriptional terminator by crossover PCR ) RFP-RXO GATGCCTGGCTCTAGTATTATTAAGCACCGGTGG 34
Plac (r0011; fwd; for adding lac promoter and RBS to ORFs by PCR) Plac-RBS-F GAATTCGCGGCCGCTTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAACAAGATACTGAGCAC ATACTAGAGAAAGAGGAGAAATACTAG 103


Retrieved from "http://2008.igem.org/Team:Waterloo/Notebook/Oligos"