Team:Chiba/Calendar-Home/24 August 2008

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Line 28: Line 28:
</tr>
</tr>
<tr>
<tr>
-
<td>[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)</td>
+
<td>[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)(&mu;L)</td>
<td>1</td><td>-</td><td>-</td><td>-</td>
<td>1</td><td>-</td><td>-</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)</td>
+
<td>[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)(&mu;L)</td>
<td>-</td><td>1</td><td>-</td><td>-</td>
<td>-</td><td>1</td><td>-</td><td>-</td>
</tr><tr>
</tr><tr>
-
<td>[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)</td>
+
<td>[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)(&mu;L)</td>
<td>-</td><td>-</td><td>1</td><td>-</td>
<td>-</td><td>-</td><td>1</td><td>-</td>
</tr><tr>
</tr><tr>
-
<td>[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)</td>
+
<td>[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)(&mu;L)</td>
<td>-</td><td>-</td><td>-</td><td>1</td>
<td>-</td><td>-</td><td>-</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ</td>
+
<td>EcoRI(&mu;L)</td>
<td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
<td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Buffer 4</td>
+
<td>Buffer 4(&mu;L)</td>
<td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td>
<td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>8</td><td>8</td><td>8</td><td>8</td>
<td>8</td><td>8</td><td>8</td><td>8</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
<td>10</td><td>10</td><td>10</td><td>10</td>
<td>10</td><td>10</td><td>10</td><td>10</td>
</tr>
</tr>
Line 71: Line 71:
</tr>
</tr>
<tr>
<tr>
-
<td>Sample DNA</td>
+
<td>Sample DNA(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye</td>
+
<td>Loading Dye(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>4</td>
<td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
<td>6</td>
<td>6</td>
</tr>
</tr>
Line 106: Line 106:
</tr>
</tr>
<tr>
<tr>
-
<td>Sample DNA</td>
+
<td>Sample DNA(&mu;L)</td>
<td>9</td>
<td>9</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye</td>
+
<td>Loading Dye(&mu;L)</td>
<td>2</td></tr>
<td>2</td></tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
<td>12</td>
<td>12</td>
</tr>
</tr>
Line 135: Line 135:
[[Team:Chiba/protocol/digestion|Digestion]]
[[Team:Chiba/protocol/digestion|Digestion]]
-
*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](1)①
+
*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](1)
-
*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2)②
+
*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2)
-
*Menbren Venus YFP③
+
*Menbren Venus YFP(3)
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
<td width="257">Sample No.</td>
<td width="257">Sample No.</td>
-
<td>①</td><td>②</td><td>③</td>
+
<td>1</td><td>2</td><td>3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA template</td>
+
<td>DNA template(&mu;L)</td>
<td>3</td><td>3</td><td>3</td>
<td>3</td><td>3</td><td>3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Buffer3</td>
+
<td>Buffer3(&mu;L)</td>
<td>1</td><td>1</td><td>1</td>
<td>1</td><td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>6</td><td>6</td><td>6</td>
<td>6</td><td>6</td><td>6</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ</td>
+
<td>EcoRI(&mu;L)</td>
<td>0.5</td><td>0.5</td><td>0.5</td>
<td>0.5</td><td>0.5</td><td>0.5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
-
<td>10μl</td><td>10μl</td><td>10μl</td>
+
<td>10</td><td>10</td><td>10</td>
</tr>
</tr>
</table>
</table>
Line 173: Line 173:
<tr>
<tr>
<td width="257">Sample No.</td>
<td width="257">Sample No.</td>
-
<td>①(afetr digestion)</td><td>②(after digestion)</td><td>③(afetr digestion)</td>
+
<td>1(afetr digestion)</td><td>2(after digestion)</td><td>3(afetr digestion)</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA tamplate</td>
+
<td>DNA tamplate(&mu;L)</td>
<td>10</td><td>10</td><td>10</td>
<td>10</td><td>10</td><td>10</td>
</tr>
</tr>
<tr>
<tr>
-
<td>loading Dye</td>
+
<td>loading Dye(&mu;L)</td>
<td>2</td><td>2</td><td>2</td>
<td>2</td><td>2</td><td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
-
<td>12μl</td><td>12μl</td><td>12μl</td>
+
<td>12</td><td>12</td><td>12</td>
</tr>
</tr>
</table>
</table>
Line 193: Line 193:
<tr>
<tr>
<td width="257">Sample No.</td>
<td width="257">Sample No.</td>
-
<td>①</td><td>②</td><td>③</td>
+
<td>1</td><td>2</td><td>3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA tamplate</td>
+
<td>DNA tamplate(&mu;L)</td>
<td>2</td><td>2</td><td>2</td>
<td>2</td><td>2</td><td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>loading Dye</td>
+
<td>loading Dye(&mu;L)</td>
<td>1</td><td>1</td><td>1</td>
<td>1</td><td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>3</td><td>3</td><td>3</td>
<td>3</td><td>3</td><td>3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
-
<td>6μl</td><td>6μl</td><td>6μl</td>
+
<td>6</td><td>6</td><td>6</td>
</tr>
</tr>
</table>
</table>
-->135V 30min
-->135V 30min

Latest revision as of 06:07, 30 October 2008

>Home | Notebook

23 August 2008 <|> 25 August 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

(22/8)--->Mini prep
  1. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
  2. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)
  3. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)
  4. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006)


--->Digestion test
  1. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
  2. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)
  3. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)
  4. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006)


Sample No. 5678
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)(μL) 1---
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)(μL) -1--
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)(μL) --1-
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)(μL) ---1
EcoRI(μL) 0.10.10.10.1
Buffer 4(μL) 0.90.90.90.9
dH2O(μL) 8888
TOTAL(μL) 10101010


--->Gel Check
Chiba-0824-1.JPG
Sample No. 1~8
Sample DNA(μL) 1
Loading Dye(μL) 1
dH2O(μL) 4
TOTAL(μL) 6
From left;
  1. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> ?
  2. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006) -> OK
  3. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) -> OK
  4. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006) -> OK
  5. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
  6. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006) -> OK
  7. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) -> OK
  8. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006) -> None?
Chiba-0824-2.JPG
Sample No. 5~8
Sample DNA(μL) 9
Loading Dye(μL) 2
dH2O(μL) 1
TOTAL(μL) 12

From the left; （single digestuion）

5, [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
6, [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006) -> OK
7, [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) -> 3400bp:Bad, 2200bp:OK
8, [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006) -> OK

Team:Output

Transformation

  • [http://partsregistry.org/Part:BBa_J63001 BBa_J63001]-->colony

Digestion

  • [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](1)
  • [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2)
  • Menbren Venus YFP(3)
Sample No. 123
DNA template(μL) 333
Buffer3(μL) 111
dH2O(μL) 666
EcoRI(μL) 0.50.50.5
TOTAL(μL) 101010


-->37°C 30min


Sample No. 1(afetr digestion)2(after digestion)3(afetr digestion)
DNA tamplate(μL) 101010
loading Dye(μL) 222
TOTAL(μL) 121212


Sample No. 123
DNA tamplate(μL) 222
loading Dye(μL) 111
dH2O(μL) 333
TOTAL(μL) 666

-->135V 30min

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/24_August_2008"