From 2008.igem.org
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| - | {{Imperial/StartPage2}}
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| - | {{Imperial/Box1|Modelling the repression of the promoter
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| - | IPTG is an inducer which binds to LacI.
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| - | LacI is a repressor that is constitutively expressed. It binds to the promoter preventing the promoter from expressing an output, in this case GFP.
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| - | In the absence of IPTG , LacI is constitutively expressed. Hence the constitutive expression model from the previous characterisation step can be assumed. At the same time, it can bind to the promoter to repress it.
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| - | [[Image:Repression.JPG|600px]]
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| - | [[Image:Equations_plac.JPG|600px]]
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| - | '''Evolution of LacI concentration'''
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| - | [[Image:Conc_LacI.jpg|300px]]
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| - | The repressor only binds to the promoter when it is in its unbound form.
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| - | '''Evolution of promoter concentration'''
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| - | [[Image:Free_promoters.jpg|300px]]
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| - | '''Evolution of the promoter-LacI complex'''
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| - | [[Image:Conc_PLacI_Complex.jpg|300px]]
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| - | '''Evolution of GFP'''
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| - | [[Image:conc_FP.jpg|300px]]
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| - | d<sub>GFP</sub>[GFP] is the basal rate of GFP. This is caused by the leakiness of the LacI-PLac complex when LacI does not repress the promoter completely.
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| - | }}
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| - | {{Imperial/EndPage|Growth_Curve|Motility}}
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Latest revision as of 03:03, 30 October 2008
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