Team:Chiba/Calendar-Home/18 September 2008

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Line 13: Line 13:
**E.coli strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
**E.coli strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
**temparature<br>37&deg;C  and 30&deg;C
**temparature<br>37&deg;C  and 30&deg;C
 +
===Team:Output===
===Team:Output===
-
[[Team:Chiba/protocol/ligation/dephosphorylation|SAP]] 1μL
+
[[Team:Chiba/protocol/ligation/dephosphorylation|SAP]] 1&mu;L
->left for:
->left for:
Line 28: Line 29:
[[Team:Chiba/protocol/ligation/ligation|Ligation]]
[[Team:Chiba/protocol/ligation/ligation|Ligation]]
*vector:[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
*vector:[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
-
*insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119](ptet+LuxR)
+
*insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119]
-
*Ligation product(ptet+LasR,ptet+RhlR,ptet+cinR)
+
*insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084004 K084004]
 +
*insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084005 K084005]
 +
*insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084006 K084006]
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
Line 63: Line 66:
[[Team:Chiba/protocol/transformation|transformation]]
[[Team:Chiba/protocol/transformation|transformation]]
-
*[http://partsregistry.org/Part:BBa_J54103 BBa_J54103](RBS+GFP)
+
*[http://partsregistry.org/Part:BBa_J54103 BBa_J54103]
*[http://partsregistry.org/Part:BBa_P2006 BBa_P2006]
*[http://partsregistry.org/Part:BBa_P2006 BBa_P2006]
[[Team:Chiba/protocol/gelcheck|Gel check]]
[[Team:Chiba/protocol/gelcheck|Gel check]]
-
*Ligation product(Venus YFP)
+
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003]
*[http://partsregistry.org/Part:BBa_J37033 BBa_J37033]
*[http://partsregistry.org/Part:BBa_J37033 BBa_J37033]
*[http://partsregistry.org/Part:BBa_J37020 BBa_J37020]
*[http://partsregistry.org/Part:BBa_J37020 BBa_J37020]

Latest revision as of 00:01, 30 October 2008

>Home | Notebook

17 September 2008 <|> 19 September 2008

Laboratory work

Team:Communication

  • time-delay test
    • transformants
      • [http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
      • [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
      • [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
    • E.coli strain
      Senders:XL10GOLD
      Receiver:JW1908
    • temparature
      37°C and 30°C


Team:Output

SAP 1μL

->left for:

  1. 1 hour at 37 °C
  2. 30 minutes at 65 °C

Zymoclean

->eluted with 25μL of NFW.

Ligation

  • vector:[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
  • insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119]
  • insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084004 K084004]
  • insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084005 K084005]
  • insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084006 K084006]
Sample No. Vector&insertVector
DNA(vector)(μL) 3.53.5
DNA(insert)(μL) 7.5-
Ligase(μL) 11
Ligase Buffer(μL) 44
dH2O(μL) 44
TOTAL(μL) 2020

transformation

  • [http://partsregistry.org/Part:BBa_J54103 BBa_J54103]
  • [http://partsregistry.org/Part:BBa_P2006 BBa_P2006]

Gel check

  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003]
  • [http://partsregistry.org/Part:BBa_J37033 BBa_J37033]
  • [http://partsregistry.org/Part:BBa_J37020 BBa_J37020]
  • [http://partsregistry.org/Part:BBa_J37032 BBa_J37032]
Sample No. each
DNA(μL) each
Loading Dye(μL) 1
dH2O(μL) 4
TOTAL(μL) 6
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