Team:Chiba/Calendar-Home/24 August 2008

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(Difference between revisions)

Latest revision as of 06:07, 30 October 2008

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23 August 2008 <|> 25 August 2008

Laboratory work

Team:Input

no work

Team:Communication

(22/8)--->Mini prep

[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006)

--->Digestion test

[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006)

Sample No.	5	6	7	8
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)(μL)	1	-	-	-
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)(μL)	-	1	-	-
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)(μL)	-	-	1	-
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)(μL)	-	-	-	1
EcoRI(μL)	0.1	0.1	0.1	0.1
Buffer 4(μL)	0.9	0.9	0.9	0.9
dH₂O(μL)	8	8	8	8
TOTAL(μL)	10	10	10	10

--->Gel Check

Sample No.	1~8
Sample DNA(μL)	1
Loading Dye(μL)	1
dH₂O(μL)	4
TOTAL(μL)	6

From left;

[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> ?
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006) -> OK
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) -> OK
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006) -> OK
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006) -> OK
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) -> OK
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006) -> None?

Sample No.	5~8
Sample DNA(μL)	9
Loading Dye(μL)	2
dH₂O(μL)	1
TOTAL(μL)	12

From the left; （single digestuion）

5, [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK

6, [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006) -> OK

7, [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) -> 3400bp:Bad, 2200bp:OK

8, [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006) -> OK

Team:Output

Transformation

[http://partsregistry.org/Part:BBa_J63001 BBa_J63001]-->colony

Digestion

[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](1)
[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2)
Menbren Venus YFP(3)

Sample No.	1	2	3
DNA template(μL)	3	3	3
Buffer3(μL)	1	1	1
dH₂O(μL)	6	6	6
EcoRI(μL)	0.5	0.5	0.5
TOTAL(μL)	10	10	10

-->37°C 30min

Sample No.	1(afetr digestion)	2(after digestion)	3(afetr digestion)
DNA tamplate(μL)	10	10	10
loading Dye(μL)	2	2	2
TOTAL(μL)	12	12	12

Sample No.	1	2	3
DNA tamplate(μL)	2	2	2
loading Dye(μL)	1	1	1
dH₂O(μL)	3	3	3
TOTAL(μL)	6	6	6

-->135V 30min

@@ Line 28: / Line 28: @@
 </tr>
 <tr>
-<td>[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)</td>
+<td>[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)(&mu;L)</td>
 <td>1</td><td>-</td><td>-</td><td>-</td>
 </tr>
 <tr>
-<td>[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)</td>
+<td>[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)(&mu;L)</td>
 <td>-</td><td>1</td><td>-</td><td>-</td>
 </tr><tr>
-<td>[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)</td>
+<td>[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)(&mu;L)</td>
 <td>-</td><td>-</td><td>1</td><td>-</td>
 </tr><tr>
-<td>[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)</td>
+<td>[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)(&mu;L)</td>
 <td>-</td><td>-</td><td>-</td><td>1</td>
 </tr>
 <tr>
-<td>EcoRI</td>
+<td>EcoRI(&mu;L)</td>
 <td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
 </tr>
 <tr>
-<td>Buffer 4</td>
+<td>Buffer 4(&mu;L)</td>
 <td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>8</td><td>8</td><td>8</td><td>8</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(&mu;L)</td>
-<td>10μl</td><td>10μl</td><td>10μl</td><td>10μl</td>
+<td>10</td><td>10</td><td>10</td><td>10</td>
 </tr>
 </table>
@@ Line 71: / Line 71: @@
 </tr>
 <tr>
-<td>Sample DNA</td>
+<td>Sample DNA(&mu;L)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>Loading Dye</td>
+<td>Loading Dye(&mu;L)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>4</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(&mu;L)</td>
-<td>6μl</td>
+<td>6</td>
 </tr>
 </table>
@@ Line 106: / Line 106: @@
 </tr>
 <tr>
-<td>Sample DNA</td>
+<td>Sample DNA(&mu;L)</td>
 <td>9</td>
 </tr>
 <tr>
-<td>Loading Dye</td>
+<td>Loading Dye(&mu;L)</td>
 <td>2</td></tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(&mu;L)</td>
-<td>12μl</td>
+<td>12</td>
 </tr>
 </table>
@@ Line 135: / Line 135: @@
 [[Team:Chiba/protocol/digestion|Digestion]]
-*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](1)①
+*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](1)
-*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2)②
+*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2)
-*Menbren Venus YFP③
+*Menbren Venus YFP(3)
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
 <td width="257">Sample No.</td>
-<td>①</td><td>②</td><td>③</td>
+<td>1</td><td>2</td><td>3</td>
 </tr>
 <tr>
-<td>DNA template</td>
+<td>DNA template(&mu;L)</td>
 <td>3</td><td>3</td><td>3</td>
 </tr>
 <tr>
-<td>Buffer3</td>
+<td>Buffer3(&mu;L)</td>
 <td>1</td><td>1</td><td>1</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>6</td><td>6</td><td>6</td>
 </tr>
 <tr>
-<td>EcoRⅠ</td>
+<td>EcoRI(&mu;L)</td>
 <td>0.5</td><td>0.5</td><td>0.5</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(&mu;L)</td>
-<td>10μl</td><td>10μl</td><td>10μl</td>
+<td>10</td><td>10</td><td>10</td>
 </tr>
 </table>
@@ Line 173: / Line 173: @@
 <tr>
 <td width="257">Sample No.</td>
-<td>①(afetr digestion)</td><td>②(after digestion)</td><td>③(afetr digestion)</td>
+<td>1(afetr digestion)</td><td>2(after digestion)</td><td>3(afetr digestion)</td>
 </tr>
 <tr>
-<td>DNA tamplate</td>
+<td>DNA tamplate(&mu;L)</td>
 <td>10</td><td>10</td><td>10</td>
 </tr>
 <tr>
-<td>loading Dye</td>
+<td>loading Dye(&mu;L)</td>
 <td>2</td><td>2</td><td>2</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(&mu;L)</td>
-<td>12μl</td><td>12μl</td><td>12μl</td>
+<td>12</td><td>12</td><td>12</td>
 </tr>
 </table>
@@ Line 193: / Line 193: @@
 <tr>
 <td width="257">Sample No.</td>
-<td>①</td><td>②</td><td>③</td>
+<td>1</td><td>2</td><td>3</td>
 </tr>
 <tr>
-<td>DNA tamplate</td>
+<td>DNA tamplate(&mu;L)</td>
 <td>2</td><td>2</td><td>2</td>
 </tr>
 <tr>
-<td>loading Dye</td>
+<td>loading Dye(&mu;L)</td>
 <td>1</td><td>1</td><td>1</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>3</td><td>3</td><td>3</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(&mu;L)</td>
-<td>6μl</td><td>6μl</td><td>6μl</td>
+<td>6</td><td>6</td><td>6</td>
 </tr>
 </table>
 -->135V 30min