Team:Chiba/Calendar-Home/26 September 2008

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===Team:Output===
===Team:Output===
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<br>colony [[Team:Chiba/protocol/PCR|PCR]]
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<br>TIME DELAY TEST
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<br>Vector:[http://partsregistry.org/Part:BBa_R0062 BBa_R0062] Insert:[http://partsregistry.org/Part:BBa_E0240 BBa_E0240]+[http://partsregistry.org/Part:BBa_R0062 BBa_R0062]&times;7
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<br>colony PCR
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<br>Ligation product(26,September)&times;7
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<br>Vector:BBa_R0062
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<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
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</tr>
</tr>
<tr>
<tr>
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<td>dH2O</td>
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<td>dH<sub>2</sub>O(&mu;L)</td>
<td>19.8</td>
<td>19.8</td>
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</tr>
<tr>
<tr>
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<td>TOTAL</td>
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<td>TOTAL(&mu;L)</td>
<td>30</td>
<td>30</td>
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<br>->95&deg;C 5 min ->(95&deg;C 1 min 50&deg;C 30 sec　72&deg;C 1 min)&times;25 ->72&deg;C 10 min
<br>->95&deg;C 5 min ->(95&deg;C 1 min 50&deg;C 30 sec　72&deg;C 1 min)&times;25 ->72&deg;C 10 min
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<br>->Gel check
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<br>->[[Team:Chiba/protocol/gelcheck|Gel check]]
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<br>->MINI Prep
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<br>->[[Team:Chiba/protocol/DNA Purification/sigma|MINI Prep]]

Latest revision as of 01:08, 30 October 2008

>Home | Notebook

25 September 2008 <|> 27 September 2008

Laboratory work

Team:Communication

Team:Output


colony PCR
Vector:[http://partsregistry.org/Part:BBa_R0062 BBa_R0062] Insert:[http://partsregistry.org/Part:BBa_E0240 BBa_E0240]+[http://partsregistry.org/Part:BBa_R0062 BBa_R0062]×7

Sample No. each
culture(μL) 1
Fwd primer(μL) 1.5
Rev primer(μL) 1.5
Taq(DNA pol)(μL) 0.2
dNTPmix(μL) 3
thermo pol Buffer(μL) 3
dH2O(μL) 19.8
TOTAL(μL) 30


->95°C 5 min ->(95°C 1 min 50°C 30 sec　72°C 1 min)×25 ->72°C 10 min
->Gel check
->MINI Prep

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