Team:Chiba/Calendar-Home/26 September 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Output)
(→Team:Output)
 
(One intermediate revision not shown)
Line 7: Line 7:
===Team:Output===
===Team:Output===
-
 
+
<br>colony [[Team:Chiba/protocol/PCR|PCR]]
-
<br>TIME DELAY TEST
+
<br>Vector:[http://partsregistry.org/Part:BBa_R0062 BBa_R0062] Insert:[http://partsregistry.org/Part:BBa_E0240 BBa_E0240]+[http://partsregistry.org/Part:BBa_R0062 BBa_R0062]&times;7
-
 
+
-
<br>colony PCR
+
-
<br>Ligation product(26,September)&times;7
+
-
<br>Vector:BBa_R0062
+
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
Line 53: Line 49:
<br>->95&deg;C 5 min ->(95&deg;C 1 min 50&deg;C 30 sec　72&deg;C 1 min)&times;25 ->72&deg;C 10 min
<br>->95&deg;C 5 min ->(95&deg;C 1 min 50&deg;C 30 sec　72&deg;C 1 min)&times;25 ->72&deg;C 10 min
-
<br>->Gel check
+
<br>->[[Team:Chiba/protocol/gelcheck|Gel check]]
-
<br>->MINI Prep
+
<br>->[[Team:Chiba/protocol/DNA Purification/sigma|MINI Prep]]

Latest revision as of 01:08, 30 October 2008

>Home | Notebook

25 September 2008 <|> 27 September 2008

Laboratory work

Team:Communication

Team:Output


colony PCR
Vector:[http://partsregistry.org/Part:BBa_R0062 BBa_R0062] Insert:[http://partsregistry.org/Part:BBa_E0240 BBa_E0240]+[http://partsregistry.org/Part:BBa_R0062 BBa_R0062]×7

Sample No. each
culture(μL) 1
Fwd primer(μL) 1.5
Rev primer(μL) 1.5
Taq(DNA pol)(μL) 0.2
dNTPmix(μL) 3
thermo pol Buffer(μL) 3
dH2O(μL) 19.8
TOTAL(μL) 30


->95°C 5 min ->(95°C 1 min 50°C 30 sec　72°C 1 min)×25 ->72°C 10 min
->Gel check
->MINI Prep

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/26_September_2008"