User:University of Washington/25 June 2008

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(Yeast Shuttle Plasmid)
 
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== Yeast Shuttle Plasmid ==
== Yeast Shuttle Plasmid ==
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- A plate of yeast strain BY4741 (MATalpha, ΔBar1::Kan MX, his3Δ1; leu2Δ0; met15Δ0; ura3Δ0) was obtained.
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- An agar plate containing yeast strain BY4741 (MATalpha, ΔBar1::Kan MX, his3Δ1; leu2Δ0; met15Δ0; ura3Δ0) was obtained.
- The yeast was incubated overnight at 30 degrees Celsius.
- The yeast was incubated overnight at 30 degrees Celsius.
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-Obtained BBa_R0040 (TetR), BBa_R0080 (AraC)
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- Obtained BBa_R0040 (TetR), BBa_R0080 (AraC)
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-transformed and plated with Amp
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- transformed and plated with Amp
== LuxR under pLac ==
== LuxR under pLac ==
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-Obtained parts R0010, I0462, and J04430.
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- Obtained parts R0010, I0462, and J04430.
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-R0010 and I0462 were transformed into MG strains, and plated on Amp.
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- R0010 and I0462 were transformed into MG strains, and plated on Amp.
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-J04430 was transformed into DH strain, and plated on Amp.
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- J04430 was transformed into DH strain, and plated on Amp.
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[[Team:University_of_Washington/Notebook#Notebook]]
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Back to [[Team:University_of_Washington/Notebook#Notebook]]

Latest revision as of 00:25, 1 July 2008

Yeast Shuttle Plasmid

- An agar plate containing yeast strain BY4741 (MATalpha, ΔBar1::Kan MX, his3Δ1; leu2Δ0; met15Δ0; ura3Δ0) was obtained.

- The yeast was incubated overnight at 30 degrees Celsius.

LuxR under AraC and TetR

- Obtained BBa_R0040 (TetR), BBa_R0080 (AraC)

- transformed and plated with Amp

LuxR under pLac

- Obtained parts R0010, I0462, and J04430.

- R0010 and I0462 were transformed into MG strains, and plated on Amp.

- J04430 was transformed into DH strain, and plated on Amp.



Back to Team:University_of_Washington/Notebook#Notebook