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Wiki/Team:Warsaw/igem notebook.htm
From 2008.igem.org
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| - | <td align=center width=70%><h1><p style="font-size: 16px; text-align: center; text-decoration: bold;">Table of contents</p></h1></td><td> </td></tr> | + | <td align=center width=70%><h1><p style="font-size: 16px; text-align: center; text-decoration: bold;">Table of contents </p></h1></td><td> </td></tr> |
<td width=70% valign=top style="border-left-style: grooved"> | <td width=70% valign=top style="border-left-style: grooved"> | ||
<h3 onClick=switchMenu('one-armed-vect') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';"> Preparation of one-armed bandit vectors</h3> | <h3 onClick=switchMenu('one-armed-vect') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';"> Preparation of one-armed bandit vectors</h3> | ||
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=7_July_2008&arg1=8_July_2008&arg2=14_July_2008&arg3=15_July_2008&arg4=16_July_2008&arg5=21_July_2008&arg6=22_July_2008&arg7=23_July_2008&arg8=28_July_2008&name=Cloning%20omega-A%20fusion%20on%20pKS%20%28second%20attempt%29>Cloning omega-A fusion on pKS (second attempt)</a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=7_July_2008&arg1=8_July_2008&arg2=14_July_2008&arg3=15_July_2008&arg4=16_July_2008&arg5=21_July_2008&arg6=22_July_2008&arg7=23_July_2008&arg8=28_July_2008&name=Cloning%20omega-A%20fusion%20on%20pKS%20%28second%20attempt%29>Cloning omega-A fusion on pKS (second attempt)</a></li> | ||
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Preparation%20of%20construct%20pKS%20with%20A%20protein>Preparation of construct pKS with A protein </a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Preparation%20of%20construct%20pKS%20with%20A%20protein>Preparation of construct pKS with A protein </a></li> | ||
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=21_July_2008&arg1=22_July_2008&arg2=23_July_2008&arg3=24_July_2008&arg4=25_July_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20OmpA%20constructs>Cloning of protein A DNA to OmpA constructs </a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=21_July_2008&arg1=22_July_2008&arg2=23_July_2008&arg3=24_July_2008&arg4=25_July_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20OmpA%20constructs>Cloning of protein A DNA to OmpA constructs </a></li> | ||
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=22_September_2008&arg1=23_September_2008&arg2=24_September_2008&arg3=25_September_2008&arg4=26_September_2008&arg5=27_September_2008&arg6=30_September_2008&name=Mutagenesis%20of%20protein%20A">Mutagenesis of protein A</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=22_September_2008&arg1=23_September_2008&arg2=24_September_2008&arg3=25_September_2008&arg4=26_September_2008&arg5=27_September_2008&arg6=30_September_2008&name=Mutagenesis%20of%20protein%20A">Mutagenesis of protein A</a></li> | ||
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| + | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=14_July_2008&arg1=15_July_2008&name=Preparation%20of%20alpha%20A%20conctruct>Preparation of alpha A construct #1</a></li> | ||
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=24_September_2008&arg1=25_September_2008&name=Preparation%20of%20alpha_A%20construct">Preparation of alpha_A construct #2</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=24_September_2008&arg1=25_September_2008&name=Preparation%20of%20alpha_A%20construct">Preparation of alpha_A construct #2</a></li> | ||
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<div name="submenu_item" id="delta_A" style="display: none"> | <div name="submenu_item" id="delta_A" style="display: none"> | ||
<ul> | <ul> | ||
| + | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=30_July_2008&arg1=31_July_2008&arg2=1_August_2008&arg3=4_August_2008&arg4=5_August_2008&name=Cloning%20of%20truncated%20fragment%20of%20protein%20A%20%28%26Delta%3BA%29>Cloning of truncated fragment of protein A (ΔA) </a></li> | ||
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=29_July_2008&arg1=30_July_2008&arg2=31_July_2008&name=Cloning%20of%20omega_%26Delta%3BA%20DNA%20fragment%20to%20pACYC177%20OmpA_alpha>Cloning of omega_ΔA DNA fragment to pACYC177 OmpA_alpha </a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=29_July_2008&arg1=30_July_2008&arg2=31_July_2008&name=Cloning%20of%20omega_%26Delta%3BA%20DNA%20fragment%20to%20pACYC177%20OmpA_alpha>Cloning of omega_ΔA DNA fragment to pACYC177 OmpA_alpha </a></li> | ||
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&arg3=7_August_2008&name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct>Preparing pACYC177 OmpA_omega_ΔA construct </a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&arg3=7_August_2008&name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct>Preparing pACYC177 OmpA_omega_ΔA construct </a></li> | ||
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</ul> | </ul> | ||
| + | </div> | ||
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=14_July_2008&arg1=15_July_2008&arg2=16_July_2008&arg3=17_July_2008&arg4=18_July_2008&arg5=19_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20OmpA%20constructs>Cloning of protein Z DNA to OmpA constructs </a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=14_July_2008&arg1=15_July_2008&arg2=16_July_2008&arg3=17_July_2008&arg4=18_July_2008&arg5=19_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20OmpA%20constructs>Cloning of protein Z DNA to OmpA constructs </a></li> | ||
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=13_August_2008&arg1=14_August_2008&arg2=18_August_2008&arg3=19_August_2008&arg4=20_August_2008&arg5=21_August_2008&arg6=22_August_2008&arg7=25_August_2008&arg8=26_August_2008&name=Cloning%20of%20alpha%20to%20pACYC177%20OmpA_A_omega%20and%20pACYC177%20OmpA_Z_omega">Cloning of alpha to pACYC177 OmpA_A_omega and pACYC177 OmpA_Z_omega</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=13_August_2008&arg1=14_August_2008&arg2=18_August_2008&arg3=19_August_2008&arg4=20_August_2008&arg5=21_August_2008&arg6=22_August_2008&arg7=25_August_2008&arg8=26_August_2008&name=Cloning%20of%20alpha%20to%20pACYC177%20OmpA_A_omega%20and%20pACYC177%20OmpA_Z_omega">Cloning of alpha to pACYC177 OmpA_A_omega and pACYC177 OmpA_Z_omega</a></li> | ||
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=17_July_2008&arg1=18_July_2008&arg2=21_July_2008&arg3=22_July_2008&arg4=23_July_2008&arg5=24_July_2008&arg6=25_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-omega%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA </a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=17_July_2008&arg1=18_July_2008&arg2=21_July_2008&arg3=22_July_2008&arg4=23_July_2008&arg5=24_July_2008&arg6=25_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-omega%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA </a></li> | ||
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<div name="menu_item" id="hunter-tests" style="display: none"> | <div name="menu_item" id="hunter-tests" style="display: none"> | ||
<ol> | <ol> | ||
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<li class="nb_toc1" OnClick="switchMenu('resist-tests','submenu_item')">Ampicillin-resistance tests</li> | <li class="nb_toc1" OnClick="switchMenu('resist-tests','submenu_item')">Ampicillin-resistance tests</li> | ||
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</ul> | </ul> | ||
</div> | </div> | ||
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| + | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=4_August_2008&arg1=5_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20expression>Checking OmpA_omega_ΔA_alpha expression</a></li> | ||
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=17_September_2008&arg1=18_September_2008&name=Optimisation%20of%20primers%20for%20preparation%20of%20parts">Optimisation of primers for preparation of parts</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=17_September_2008&arg1=18_September_2008&name=Optimisation%20of%20primers%20for%20preparation%20of%20parts">Optimisation of primers for preparation of parts</a></li> | ||
| - | <li class="nb_toc1" | + | <li class="nb_toc1" OnClick=switchMenu('bricks-vect','submenu_item')>Preparation of vectors for BioBricks parts cloning</li> |
<div name="submenu_item" id="bricks-vect" style="display: none"> | <div name="submenu_item" id="bricks-vect" style="display: none"> | ||
<ul> | <ul> | ||
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</div> | </div> | ||
| - | <li class="nb_toc1" OnClick=switchMenu('bricks-coding','submenu_item')>Preparation of BioBricks parts (coding type) | + | <li class="nb_toc1" OnClick=switchMenu('bricks-coding','submenu_item')>Preparation of BioBricks parts (coding type)</li> |
<div name="submenu_item" id="bricks-coding" style="display: none"> | <div name="submenu_item" id="bricks-coding" style="display: none"> | ||
<ul> | <ul> | ||
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</div> | </div> | ||
| - | <li class="nb_toc1" OnClick=switchMenu('bricks-generator','submenu_item')>Preparation of BioBricks parts (coding type) | + | <li class="nb_toc1" OnClick=switchMenu('bricks-generator','submenu_item')>Preparation of BioBricks parts (coding type)</li> |
<div name="submenu_item" id="bricks-generator" style="display: none"> | <div name="submenu_item" id="bricks-generator" style="display: none"> | ||
<ul> | <ul> | ||
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=8_September_2008&name=Our%20webpage%20has%20a%20completely%20new%20layout">Our webpage has a completely new layout</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=8_September_2008&name=Our%20webpage%20has%20a%20completely%20new%20layout">Our webpage has a completely new layout</a></li> | ||
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=11_September_2008&name=We%27re%20fighting%20hard%20to%20obtain%20funds%20for%20the%20visit%20on%20iGEM2008%20Jamboree%21">We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=11_September_2008&name=We%27re%20fighting%20hard%20to%20obtain%20funds%20for%20the%20visit%20on%20iGEM2008%20Jamboree%21">We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</a></li> | ||
| + | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=10_October_2008&name=Visit%20in%20US%20Embassy">Visit in US Embassy</a></li> | ||
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=23_October_2008&name=Sending%20our%20bricks%20to%20the%20Registry">Sending our bricks to the Registry</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=23_October_2008&name=Sending%20our%20bricks%20to%20the%20Registry">Sending our bricks to the Registry</a></li> | ||
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=24_October_2008&name=Computer%20meeting%20to%20finish%20our%20webpage">Computer meeting to finish our webpage</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=24_October_2008&name=Computer%20meeting%20to%20finish%20our%20webpage">Computer meeting to finish our webpage</a></li> | ||
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</ol> | </ol> | ||
</div> | </div> | ||
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<h4><a name="top10">TOP10 (Invitrogen)</a></h4> | <h4><a name="top10">TOP10 (Invitrogen)</a></h4> | ||
<p>F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- </p> | <p>F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- </p> | ||
| - | <h4><a name="mutD5">NR9458 ( | + | <h4><a name="mutD5">NR9458 (<a href="http://www.ibb.waw.pl/">Institute of Biochemistry and Biophysics</a> collection; in notebook called mutD5)</a></h4> |
<p>ara thi Δ pro lac mutD5 zaf-13 ::Tn10</p> | <p>ara thi Δ pro lac mutD5 zaf-13 ::Tn10</p> | ||
<h4><a name="gm">GM2163</a></h4> | <h4><a name="gm">GM2163</a></h4> | ||
Latest revision as of 06:51, 30 October 2008
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ProtocolsPrimersVectorsStrainsJavaScript tools developed to make work with iGEM notebook more comfortable |
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