Team:Chiba/Calendar-Home/8 September 2008

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'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''
'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''
 +
{|align="justify"
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|[[Image:Chiba-0908.JPG|300px]]
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|
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:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
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<td width="257">Sample DNA</td>
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<td>60</td>
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</tr>
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<tr>
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<td>Loading Dye</td>
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<td>12</td>
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</tr>
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<tr>
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<td>TOTAL</td>
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<td>72μL</td>
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</tr>
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</table>
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:From left;
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::[http://partsregistry.org/Part:BBa_ BBa_](2007)
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::100 ng / 1 well
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|}
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 +
{|align="justify"
 +
|[[Image:Chiba-0908-2.JPG]]
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|
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:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
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<td width="257">Sample Name</td>
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<td>[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]</td><td>[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]</td>
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</tr>
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<tr>
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<td width="257">Sample DNA</td>
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<td>30</td><td>60</td>
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</tr>
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<tr>
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<td>Loading Dye</td>
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<td>6</td><td>12</td>
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</tr>
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<tr>
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<td>TOTAL</td>
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<td>36μL</td><td>72μL</td>
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</tr>
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</table>
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:From left;
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::[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
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::[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]
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|}
 +
 +
{|align="justify"
 +
|[[Image:Chiba-0908-3.JPG]]
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|
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:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
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<td width="257">Sample DNA</td>
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<td>100</td>
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</tr>
 +
<tr>
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<td>Loading Dye</td>
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<td>20</td>
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</tr>
 +
<tr>
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<td>TOTAL</td>
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<td>6μL</td>
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</tr>
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</table>
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::[http://partsregistry.org/Part:BBa_C0261 BBa_C0261] -> none
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|}
:--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
:--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
:::eluted with 15μL(?) of NFW.
:::eluted with 15μL(?) of NFW.
 +
 +
 +
'''[https://2008.igem.org/Team:Chiba/protocol/Time_Delay_Test|Time Delay Test]'''
===Team:Output===
===Team:Output===

Latest revision as of 23:43, 29 October 2008

>Home | Notebook

7 September 2008 <|> 9 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

-->(7/9)

Gel Extract

Chiba-0908.JPG
Sample DNA 60
Loading Dye 12
TOTAL 72μL
From left;
[http://partsregistry.org/Part:BBa_ BBa_](2007)
100 ng / 1 well
Chiba-0908-2.JPG
Sample Name [http://partsregistry.org/Part:BBa_J04500 BBa_J04500][http://partsregistry.org/Part:BBa_C0161 BBa_C0161]
Sample DNA 3060
Loading Dye 612
TOTAL 36μL72μL
From left;
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]
Chiba-0908-3.JPG
Sample DNA 100
Loading Dye 20
TOTAL 6μL
[http://partsregistry.org/Part:BBa_C0261 BBa_C0261] -> none


--->Zymo Clean
eluted with 15μL(?) of NFW.


Delay Test

Team:Output

SAP

  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1)
  • [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5)
Sample No. 15
DNA(digested sample)(μL) 76
SAP(μL) 22
SAP Buffer(μL) 33
TOTAL(μL) 3030

-->left at 37 degrees for 1 hour.

-->left at 65 degrees for 30 minutes.

-->DNA purification(1:21μl,5:6μl)


-->Gel check

Sample No. each
DNA(digested sample)(μL) 1
Loading Dye(μL) 1
dH2O(μL) 4
TOTAL(μL) 6
  • vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1) 75ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2) 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](3) 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03160 BBa_S03160](4) 38.4ng/μl
  • vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5) 100ng/μl
  • insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](6) 33.6ng/μl
Sample No. 1&21&31&415&65
DNA(vector)(μL) 333352.1
DNA(insert)(μL) 333-3.1-
Ligase(μL) 111111
Ligase Buffer(x5)(μL) 222222
dH2O(μL) 11148.94
TOTAL(μL) 101010102010
Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/8_September_2008"