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Template:Team:UC Berkeley/Notebook/CDB construction
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Product is pBca1256-K112400 | Product is pBca1256-K112400 | ||
---------------------------- | ---------------------------- | ||
| - | cb0001 grpE-F Forward Biobricking cgataGAATTCatgAGATCTccgaggtccttgttgcgaag | + | cb0001-grpE-F Forward Biobricking cgataGAATTCatgAGATCTccgaggtccttgttgcgaag |
| - | cb0002 grpE-R Reverse Biobricking cgtagGGATCCcgcattcatctcgctaacttc | + | cb0002-grpE-R Reverse Biobricking cgtagGGATCCcgcattcatctcgctaacttc |
</pre> | </pre> | ||
| Line 19: | Line 19: | ||
Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) | Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) | ||
Product is pBca1256-K112401 | Product is pBca1256-K112401 | ||
| - | + | ---------------------------- | |
| - | cb0003 recA-F Forward Biobricking cgataGAATTCatgAGATCTagagaagcctgtcggcaccg | + | cb0003-recA-F Forward Biobricking cgataGAATTCatgAGATCTagagaagcctgtcggcaccg |
| - | cb0004 recA-R Reverse Biobricking cgtagGGATCCcccagtgctgccgccaacgc | + | cb0004-recA-R Reverse Biobricking cgtagGGATCCcccagtgctgccgccaacgc |
</pre> | </pre> | ||
| Line 31: | Line 31: | ||
Product is pBca1256-K112402 | Product is pBca1256-K112402 | ||
---------------------------- | ---------------------------- | ||
| - | cb0005 fabA-F Forward Biobricking cgataGAATTCatgAGATCTggccattacgttggctgaac | + | cb0005-fabA-F Forward Biobricking cgataGAATTCatgAGATCTggccattacgttggctgaac |
| - | cb0006 fabA-R Reverse Biobricking cgtagGGATCCgaagttaccacccgtttcgg | + | cb0006-fabA-R Reverse Biobricking cgtagGGATCCgaagttaccacccgtttcgg |
| + | </pre> | ||
| + | |||
| + | ==pBca1256-K112403== | ||
| + | <pre> | ||
| + | Biobricking of signal peptide (prepro) of phoA (alkaline phosphatase) | ||
| + | PCR cb10007-F and cb10008 on MG1655 (114 bp, EcoRI/BamHI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI 910+2472bp, L) | ||
| + | Product is pBca1256-K112403 | ||
| + | ---------------------------- | ||
| + | cb10007-phoA-F cgataGAATTCatgAGATCTgtacatggagaaaataaaatgaaacaaagc | ||
| + | cb10008-phoA-R cgtagGGATCCgggcttttgtcacaggggtaaac | ||
| + | </pre> | ||
| + | |||
| + | ==pBca1256-K112404== | ||
| + | <pre> | ||
| + | Biobricking of signal peptide (prepro) of lamB protein | ||
| + | PCR cb10009-F and cb10010 on MG1655 (132 bp, EcoRI/BamHI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI 910+2472bp, L) | ||
| + | Product is pBca1256-K112404 | ||
| + | ---------------------------- | ||
| + | cb10009-lamB-F cgataGAATTCatgAGATCTgaaaagcaatgactcaggagatag | ||
| + | cb10010-lamB-R cgtagGGATCCcagccattgcctgagcagacattac | ||
| + | </pre> | ||
| + | |||
| + | ==pBca1256-K112405== | ||
| + | <pre> | ||
| + | Biobricking of promoter for cadAB genes | ||
| + | PCR cad-F and cad-R on MG1655 (402 bp, EcoRI/BamHI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI 910+2472bp, L) | ||
| + | Product is pBca1256-K112405 | ||
| + | ---------------------------- | ||
| + | cb10011-cad-F cgataGAATTCatgAGATCTggtatattccagacttctgttcc | ||
| + | cb10012-cad-R cgtagGGATCCtgaccagaccaaactggcg | ||
| + | </pre> | ||
| + | |||
| + | ==pBca1256-K112406== | ||
| + | <pre> | ||
| + | Biobricking of cadC promoter and cadC gene | ||
| + | PCR cadC-F and cadC-R on MG1655 (2336 bp, EcoR1/BamH1) | ||
| + | Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) | ||
| + | Product is pBca1256-K112406 | ||
| + | ---------------------------- | ||
| + | cb10013-cadC-F cgataGAATTCatgAGATCTcaccagcaatttgcgggcatatc | ||
| + | cb10012-cad-R cgtagGGATCCtgaccagaccaaactggcg | ||
| + | </pre> | ||
| + | |||
| + | ==pBca1256-K112407== | ||
| + | <pre> | ||
| + | Biobricking of ygeF pseudogene promoter | ||
| + | PCR ygeF-F and ygeF-R on MG1655 (540 bp, EcoR1/BamH1) | ||
| + | Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) | ||
| + | Product is pBca1256-K112407 | ||
| + | ---------------------------- | ||
| + | cb10014-ygeF-F cgataGAATTCatgAGATCTcagtttactcagtgaatcaatagagg | ||
| + | cb10015-ygeF-R cgtagGGATCCatttgattagagcaaaatccCaattcgcagc | ||
| + | </pre> | ||
| + | |||
| + | ==pBjh1601CA-K112408== | ||
| + | <pre> | ||
| + | T4 derived lysis device | ||
| + | Sub into pBjh1601KA (5025 bp, EcoR1/BamH1) | ||
| + | Product is pBjh1601KA-K112408 (EcoR1/BamH1 3196/1824 S) | ||
| + | ---------------------------- | ||
</pre> | </pre> | ||
Latest revision as of 04:22, 24 October 2008
Contents |
pBca1256-K112400
Biobricking of promoter for grpE protein PCR grpE-F and grpE-R on MG1655 (129 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI 910+2472bp, L) Product is pBca1256-K112400 ---------------------------- cb0001-grpE-F Forward Biobricking cgataGAATTCatgAGATCTccgaggtccttgttgcgaag cb0002-grpE-R Reverse Biobricking cgtagGGATCCcgcattcatctcgctaacttc
pBca1256-K112401
Biobricking of promoter for recA protein PCR recA-F and recA-R on MG1655 (317 bp, EcoR1/BamH1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112401 ---------------------------- cb0003-recA-F Forward Biobricking cgataGAATTCatgAGATCTagagaagcctgtcggcaccg cb0004-recA-R Reverse Biobricking cgtagGGATCCcccagtgctgccgccaacgc
pBca1256-K112402
Biobricking of promoter for fabA protein PCR fabA-F and fabA-R on MG1655 (285 bp, EcoR1/BamH1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112402 ---------------------------- cb0005-fabA-F Forward Biobricking cgataGAATTCatgAGATCTggccattacgttggctgaac cb0006-fabA-R Reverse Biobricking cgtagGGATCCgaagttaccacccgtttcgg
pBca1256-K112403
Biobricking of signal peptide (prepro) of phoA (alkaline phosphatase) PCR cb10007-F and cb10008 on MG1655 (114 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI 910+2472bp, L) Product is pBca1256-K112403 ---------------------------- cb10007-phoA-F cgataGAATTCatgAGATCTgtacatggagaaaataaaatgaaacaaagc cb10008-phoA-R cgtagGGATCCgggcttttgtcacaggggtaaac
pBca1256-K112404
Biobricking of signal peptide (prepro) of lamB protein PCR cb10009-F and cb10010 on MG1655 (132 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI 910+2472bp, L) Product is pBca1256-K112404 ---------------------------- cb10009-lamB-F cgataGAATTCatgAGATCTgaaaagcaatgactcaggagatag cb10010-lamB-R cgtagGGATCCcagccattgcctgagcagacattac
pBca1256-K112405
Biobricking of promoter for cadAB genes PCR cad-F and cad-R on MG1655 (402 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI 910+2472bp, L) Product is pBca1256-K112405 ---------------------------- cb10011-cad-F cgataGAATTCatgAGATCTggtatattccagacttctgttcc cb10012-cad-R cgtagGGATCCtgaccagaccaaactggcg
pBca1256-K112406
Biobricking of cadC promoter and cadC gene PCR cadC-F and cadC-R on MG1655 (2336 bp, EcoR1/BamH1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112406 ---------------------------- cb10013-cadC-F cgataGAATTCatgAGATCTcaccagcaatttgcgggcatatc cb10012-cad-R cgtagGGATCCtgaccagaccaaactggcg
pBca1256-K112407
Biobricking of ygeF pseudogene promoter PCR ygeF-F and ygeF-R on MG1655 (540 bp, EcoR1/BamH1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112407 ---------------------------- cb10014-ygeF-F cgataGAATTCatgAGATCTcagtttactcagtgaatcaatagagg cb10015-ygeF-R cgtagGGATCCatttgattagagcaaaatccCaattcgcagc
pBjh1601CA-K112408
T4 derived lysis device Sub into pBjh1601KA (5025 bp, EcoR1/BamH1) Product is pBjh1601KA-K112408 (EcoR1/BamH1 3196/1824 S) ----------------------------
