Team:Chiba/Calendar-Home/20 October 2008

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Transformation
Transformation
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#BBa_T9002(sensitive mutant LuxR)
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#[http://partsregistry.org/Part:BBa_T9002%E3%80%80BBa_T9002 BBa_T9002][https://2008.igem.org/Team:Chiba/Experiments:LuxR_mutant (sensitive mutant LuxR)]
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#BBa_T9002[http://partsregistry.org/Part:BBa_T9002]
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#[http://partsregistry.org/Part:BBa_T9002%E3%80%80BBa_T9002 BBa_T9002]
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#BBa_T9002(RBS0.6)
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#[http://partsregistry.org/Part:BBa_T9002%E3%80%80BBa_T9002 BBa_T9002](RBS0.6)
#Plac-his aiiA-T
#Plac-his aiiA-T
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Ptet-RBS-luxR-T-Plux-RBS-GFP-Cm-p15A
Ptet-RBS-luxR-T-Plux-RBS-GFP-Cm-p15A
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#Ptet-RBS-luxR-T-Plux-RBS-GFP-Cm-p15A
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#[https://2008.igem.org/Team:Chiba/Experiments:copy_number Ptet-RBS-luxR-T-Plux-RBS-GFP-Cm-p15A]
#Ptet-RBS-LuxR-Kan-P15A
#Ptet-RBS-LuxR-Kan-P15A

Latest revision as of 01:49, 30 October 2008

>Home | Notebook

19 October 2008 <|> 21 October 2008

Laboratory work

Team:Receivers

Transformation

  1. [http://partsregistry.org/Part:BBa_T9002%E3%80%80BBa_T9002 BBa_T9002](sensitive mutant LuxR)
  1. [http://partsregistry.org/Part:BBa_T9002%E3%80%80BBa_T9002 BBa_T9002]
  1. [http://partsregistry.org/Part:BBa_T9002%E3%80%80BBa_T9002 BBa_T9002](RBS0.6)
  1. Plac-his aiiA-T

Ptet-RBS-luxR-T-Plux-RBS-GFP-Cm-p15A

  1. Ptet-RBS-luxR-T-Plux-RBS-GFP-Cm-p15A
  1. Ptet-RBS-LuxR-Kan-P15A

Plux-RBS-GFP-T-Amp-colE1

  1. Ptet-RBS-LuxR-T-Plux-RBS-GFP-T-Plac-aiiA-Amp-colE1

-->Transformation


Team:Demo-Rs


  1. Receiver(T9002) pre-incubation
    1. Receiver:BBa_T9002(JW1908)wascultured in 2mL LB-Amp (37°C,12h)

~21 October

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