Team:University of Ottawa/25 June 2008

From 2008.igem.org

(Difference between revisions)
Line 14: Line 14:
::<li> Cleaned out all DNA that will not be used from our box
::<li> Cleaned out all DNA that will not be used from our box
::<li> Our names are getting complicated so I am renaming our DNA using the following legend (all other DNA was discarded)
::<li> Our names are getting complicated so I am renaming our DNA using the following legend (all other DNA was discarded)
-
:::597        > 7B   > 7
+
:::{| class="wikitable"
-
:::598         > 8B   > 8
+
|-
-
:::S1           > SB   > S
+
| '''Plasmid''' || '''Strain''' || '''New Name'''
-
:::D12       > DA   > D
+
|-
-
:::T123     > TB   > T
+
| 587 || 7B || 7
-
:::pSSA42 > 42B > 42  
+
|-
 +
| 598 || 8B || 8
 +
|-
 +
| S1 || SB || S
 +
|-
 +
| D12 || DA || D
 +
|-
 +
| T123 || TB || T
 +
|-
 +
| pSSA42 || 42B || 42
 +
|}
:'''PCR amplification of S, D, T, 7 and 8'''
:'''PCR amplification of S, D, T, 7 and 8'''
::<li> S, D, and T plasmids were PCR amplified using primers F69 and F70
::<li> S, D, and T plasmids were PCR amplified using primers F69 and F70

Revision as of 14:37, 30 June 2008

Contents

Today in the Lab

Matt

PCR Amplification
  • F60, F61 primers used for PTP2 cassette. I also labelled all the primers we received that we will be using for the next few months.
  • I decided to use the Kaern Lab protocol for PCR Amplification.
  • The PTP2 PCR product was then run on a gel - gel results were as expected with 2275 size band.
    • Glycerol Stock
  • Glycerol stock of plasmids D12, S1, T123, pSS042 with LB + glycerol was performed.

    • Dan

    Ran gel of pSSA42, S1, D12, T123 plasmids
  • This time the marker was added and expected results were obtained. SB, DA, TB, and 42B colonies were chosen for glycerol stock.
    • Cleaned out DNA box
  • Cleaned out all DNA that will not be used from our box
  • Our names are getting complicated so I am renaming our DNA using the following legend (all other DNA was discarded)
    • Plasmid Strain New Name
    587 7B 7
    598 8B 8
    S1 SB S
    D12 DA D
    T123 TB T
    pSSA42 42B 42
    PCR amplification of S, D, T, 7 and 8
  • S, D, and T plasmids were PCR amplified using primers F69 and F70
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/25_June_2008"