"
Template:Team:UC Berkeley/Notebook/CC construction
From 2008.igem.org
(Difference between revisions)
Cicikashou (Talk | contribs) (→K112506) |
Cicikashou (Talk | contribs) (→K112507) |
||
| Line 101: | Line 101: | ||
</pre> | </pre> | ||
| - | == | + | ==K112506== |
<pre> | <pre> | ||
| - | Construction of { | + | Construction of {rbs_barstar!} basic part K112506 |
| - | PCR mea035/mea034 on Bacillus amyloliquefaciens gen. ( | + | PCR mea035/mea034 on Bacillus amyloliquefaciens gen. (313 bp, EcoRI/BamHI) |
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
| - | Product is pBca1256- | + | Product is pBca1256-K112506 |
---------------------------------------- | ---------------------------------------- | ||
| - | mea035 Forward Biobricking of { | + | mea035 Forward Biobricking of {rbs_barstar!} |
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg | cgataGAATTCatgAGATCTcataagaaaggagccgcacatg | ||
| - | mea034 Reverse Biobricking of { | + | mea034 Reverse Biobricking of {rbs_barstar!} |
cgttaGGATCCttaagaaagtatgatggtgatgtcgc | cgttaGGATCCttaagaaagtatgatggtgatgtcgc | ||
</pre> | </pre> | ||
Revision as of 18:35, 2 July 2008
Contents |
K112500
Construction of {<HA>} basic part K112501
Wobble PCR of cc001/cc002 ( 50 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112500
--------------------------------------------
cc001 Forward Biobricking of {<HA>}
CgATAgaattcATGagatcttacccatacgacgtcccagac
cc002 Reverse biobricking of {<HA>}
ccagtggatccccagcgtagtctgggacgtcgtatggg
K112501
Construction of {<HA!} basic part K112502
Wobble PCR of cc001/cc003 ( 53 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112501
--------------------------------------------
cc001 Forward Biobricking of {<HA>}
CgATAgaattcATGagatcttacccatacgacgtcccagac
cc003 Reverse biobricking of {<HA!}
ccagtggatccttaccagcgtagtctgggacgtcgtatgggtaag
K112502
Construction of {<myc>} basic part K112503
Wobble PCR of cc004/cc005 ( 51 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112502
--------------------------------------------
cc004 Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc005 Reverse Biobricking of {<myc>}
ccagtggatccCAGATCCTCTTCTGAGATGAGTTTTTG
K112503
Construction of {<myc!} basic part K112503
Wobble PCR of cc004/cc006 ( 54 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI,2227+ 910,L)
Product is pBca1256-K112503
--------------------------------------------
cc004 Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc006 Reverse Biobricking of {<myc!}
ccagtggatccTTACAGATCCTCTTCTGAGATGAGTTTTTGTTC
K112504
Construction of {<barnase>} basic part K112504
PCR CC007/CC008 on barnase gene ( 351 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112504
--------------------------------------------
cc007 Forward Biobricking of {<barnase>}
CGATAgaattcATGaGATCTGCACAGG
CC008 Reverse Biobricking of {<barnase>}
CCAGTggatcCTCTGATTTTTGTAAAGGTC
K112505
Construction of {barnase>} basic part K112505
PCR CC009/CC008 on barnase gene ( 354 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112505
--------------------------------------------
cc009 Forward Biobricking of {barnase>}
CGATAgaattcATGagatctATGGCACAGGTTATCAACACGTTTG
cc008 Reverse Biobricking of {barnase>}
CCAGTggatcCTCTGATTTTTG
K112506
Construction of {<barnase!} basic part K112506
PCR CC007/CC009 on barnase gene ( 354 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112506
--------------------------------------------
cc007 Forward Biobricking of {<barnase!}
CGATAgaattcATGaGATCTGCACAGG
CC009 Reverse Biobricking of {<barnase!}
CCAGTggatcCTTATCTGATTTTTG
K112506
Construction of {rbs_barstar!} basic part K112506
PCR mea035/mea034 on Bacillus amyloliquefaciens gen. (313 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112506
----------------------------------------
mea035 Forward Biobricking of {rbs_barstar!}
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg
mea034 Reverse Biobricking of {rbs_barstar!}
cgttaGGATCCttaagaaagtatgatggtgatgtcgc
