Template:Team:UC Berkeley/Notebook/CC construction

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(Difference between revisions)
(→K112505)
(→K112504)
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Construction of {<barnase>} basic part K112504
Construction of {<barnase>} basic part K112504
-
PCR CC007/CC008 on barnase gene            ( 351 bp, EcoRI/BamHI)
+
PCR CC007/CC008 on barnase gene            ( 361 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112504
Product is pBca1256-K112504

Revision as of 18:40, 2 July 2008

Contents


K112500

Construction of {<HA>} basic part K112501

Wobble PCR of cc001/cc002                  ( 50 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112500
--------------------------------------------
cc001             Forward Biobricking of {<HA>} 
CgATAgaattcATGagatcttacccatacgacgtcccagac

cc002             Reverse biobricking of {<HA>} 
ccagtggatccccagcgtagtctgggacgtcgtatggg

K112501

Construction of {<HA!} basic part K112502

Wobble PCR of cc001/cc003                  ( 53 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112501
--------------------------------------------
cc001             Forward Biobricking of {<HA>} 
CgATAgaattcATGagatcttacccatacgacgtcccagac

cc003             Reverse biobricking of {<HA!}
ccagtggatccttaccagcgtagtctgggacgtcgtatgggtaag

K112502

Construction of {<myc>} basic part K112503

Wobble PCR of cc004/cc005                  ( 51 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112502
--------------------------------------------
cc004             Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc005             Reverse Biobricking of {<myc>}
ccagtggatccCAGATCCTCTTCTGAGATGAGTTTTTG

K112503

Construction of {<myc!} basic part K112503

Wobble PCR of cc004/cc006                  ( 54 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI,2227+ 910,L)
Product is pBca1256-K112503
--------------------------------------------
cc004             Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc006             Reverse Biobricking of {<myc!}
ccagtggatccTTACAGATCCTCTTCTGAGATGAGTTTTTGTTC

K112504

Construction of {<barnase>} basic part K112504

PCR CC007/CC008 on barnase gene            ( 361 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112504
--------------------------------------------
cc007            Forward Biobricking of {<barnase>}
CGATAgaattcATGaGATCTGCACAGG
CC008            Reverse Biobricking of {<barnase>}
CCAGTggatcCTCTGATTTTTGTAAAGGTC

K112505

Construction of {<barnase!} basic part K112505

PCR CC007/CC009 on barnase gene            ( 364 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112505
--------------------------------------------
cc007            Forward Biobricking of {<barnase!} 
CGATAgaattcATGaGATCTGCACAGG
CC009            Reverse Biobricking of {<barnase!} 
CCAGTggatcCTTATCTGATTTTTG

K112506

Construction of {rbs_barstar!} basic part K112506

PCR mea035/mea034 on Bacillus amyloliquefaciens gen.  (323 bp, EcoRI/BamHI)
Sub into pBca1256                                     (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112506
----------------------------------------
mea035       Forward Biobricking of  {rbs_barstar!}  
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg
mea034       Reverse Biobricking of  {rbs_barstar!}  
cgttaGGATCCttaagaaagtatgatggtgatgtcgc
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