Team:KULeuven/Tools/PCR

From 2008.igem.org

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|style="background:#003E81; color:white"|PCR Settings
|style="background:#003E81; color:white"|PCR Settings
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|Annealing (°C)
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| width="200" |Annealing (°C)
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|bgcolor="#66FFCC"|Buffer (5x)
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|bgcolor="#99CCFF" colspan="2"|{{{buffer}}}
|bgcolor="#99CCFF" colspan="2"|{{{buffer}}}
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|bgcolor="#99CCFF"|{{{size}}}
|bgcolor="#99CCFF"|{{{size}}}

Revision as of 16:07, 3 July 2008

PCR:
PCR Settings
Annealing (°C)
{{{tanneal}}}°
Time Elongation
{{{telong}}}
Number of Cycles
{{{ncycles}}}
Buffer (5x) {{{buffer}}}
MgCl2 10 µM {{{mgcl}}}
dNTP 10 µM {{{dntp}}}
Oligo F 10 µM {{{fwprimername}}} {{{fwprimerconc}}}
Oligo R 10 µM {{{rvprimername}}} {{{rvprimerconc}}}
Water {{{water}}}
Polymerase {{{polymerase}}}
DNA {{{dna}}}
Expected Size
{{{size}}}
Success
{{{success}}}
Image (click to enlarge)
[[Image:{{{Image}}}|30px]]
Lane (0=ladder)
{{{lane}}}