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Rensselaer/7 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: Overviewed procedure for introducing gene of interest: pick plasmid filter paper - into buffer primer design - insert restriction site (comparable with plasmid) PCR (primers, DNA polymera...) |
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pick plasmid | pick plasmid | ||
filter paper - into buffer | filter paper - into buffer | ||
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primer design - insert restriction site (comparable with plasmid) | primer design - insert restriction site (comparable with plasmid) | ||
| + | |||
PCR (primers, DNA polymerase, dNTP's,buffer) | PCR (primers, DNA polymerase, dNTP's,buffer) | ||
| + | |||
Digestion w/ restriction enzymes | Digestion w/ restriction enzymes | ||
| + | |||
Ligation (plasmid and insert) | Ligation (plasmid and insert) | ||
| + | |||
introduce to bacteria (transformation) | introduce to bacteria (transformation) | ||
Latest revision as of 19:49, 7 July 2008
Overviewed procedure for introducing gene of interest:
pick plasmid filter paper - into buffer
primer design - insert restriction site (comparable with plasmid)
PCR (primers, DNA polymerase, dNTP's,buffer)
Digestion w/ restriction enzymes
Ligation (plasmid and insert)
introduce to bacteria (transformation)
potential problem - psuedomonas - how to get plasmid DNA?
perform PCR on solution
