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Alberta NINT/7 July 2008
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| + | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| + | [[Alberta_NINT/4_July_2008 | < Previous entry]] | ||
| + | |||
| + | lab work (SD): | ||
| + | K102022.1 becomes K102022 and K102032.1 becomes K102032. | ||
| + | Transformed XL1-B cells with K102022---------------LB + amp50 + IPTG + X-Gal + 20% glucose | ||
| + | K102032---------------LB + amp50 + IPTG + X-Gal + 20% glucose | ||
| + | K102032 + K102011-----LB + amp50 + kan35 + IPTG + X-Gal + 20% glucose | ||
| + | K102032 + K102034-----LB + amp50 + kan35 + IPTG + X-Gal + 20% glucose | ||
| + | K102032 + K102035-----LB + amp50 + kan35 + IPTG + X-Gal + 20% glucose | ||
| + | K102032 + K102036-----LB + amp50 + kan35 + IPTG + X-Gal + 20% glucose | ||
| + | Negative control plates are arabinose - and test plates are arabinose + | ||
| + | |||
| + | |||
| + | [[Alberta_NINT/8_July_2008 | Next entry >]] | ||
Revision as of 21:57, 7 July 2008
lab work (SD):
K102022.1 becomes K102022 and K102032.1 becomes K102032.
Transformed XL1-B cells with K102022---------------LB + amp50 + IPTG + X-Gal + 20% glucose
K102032---------------LB + amp50 + IPTG + X-Gal + 20% glucose
K102032 + K102011-----LB + amp50 + kan35 + IPTG + X-Gal + 20% glucose
K102032 + K102034-----LB + amp50 + kan35 + IPTG + X-Gal + 20% glucose
K102032 + K102035-----LB + amp50 + kan35 + IPTG + X-Gal + 20% glucose
K102032 + K102036-----LB + amp50 + kan35 + IPTG + X-Gal + 20% glucose
Negative control plates are arabinose - and test plates are arabinose +
